Heterogeneous Responses of Gastric Cancer Cell Lines to Tenovin-6 and Synergistic Effect with Chloroquine
Abstract
:1. Introduction
2. Results
2.1. Tenovin-6 Inhibits Cell Proliferation and Anchorage-Independent Growth of GC Cells
2.2. Tenovin-6 Induces Apoptosis and Cell-Cycle Arrest of GC Cells
2.3. Tenovin-6 Initiates But Blocks Autophagy Flux in Some GC Cell Lines
2.4. Tenovin-6 Induces and Activates p53 in Some GC Cell Lines
2.5. Tenovin-6 and Chloroquine Synergistically Inhibit Cell Proliferation in GC Cells
3. Discussion
4. Materials and Methods
4.1. Reagents and Antibodies
4.2. Cell Culture
4.3. Western-Blotting
4.4. Cell Cycle and Apoptosis Assays
4.5. Colony Formation in Soft Agar
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Cell Line | Inhibition of Proliferation by Tenovin-6 | Apoptosis (%) 4 | Cell Cycle (%) 4 | TP53 Gene | Total p53 | p-p53 | Ac-p53 | p21 | LC3B | p62 | ||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Con (μM) 1 | Tm (h) 2 | Inh (%) 3 | G1 | S | G2 | |||||||||
AGS | 0.5 | 48 | 81.5 | ↑7.2 ± 4.4 | ↓13.8 ± 1.6 | ↑16.6 ± 1.9 | ↑2.7 ± 1.3 | WT | ↑ | ↑ | ↑ | ↑ | UC | UC |
AGS-EBV | 0.5 | 48 | 88.3 | ↑8.6 ± 4.3 | ↓8.9 ± 3.1 | ↑29.7 ± 3.5 | ↓15.4 ± 2.5 | WT | ↑ | ↑ | ↑ | ↑ | UC | UC |
SNU-719 | 8.0 | 48 | 76.6 | ↑6.1 ± 0.6 | ↑28.1 ± 0.7 | ↓25.0 ± 0.3 | ↓3.0 ± 1.0 | WT | ↑ | ↑ | ↑ | ↑ | ↑ | UC |
HGC-27 | 1.0 | 72 | 86.2 | ↑13.3 ± 1.3 | ↓12.2 ± 1.3 | ↑21.9 ± 0.9 | ↓9.7 ± 0.8 | Mut | ND | ND | ND | UC | ↑ | ↑ |
N87 | 3.0 | 48 | 93.3 | ↑30.9 ± 3.0 | ↑22.8 ± 1.5 | ↓20.2 ± 2.8 | ↓2.6 ± 3.4 | Mut | UC | UC | UC | UC | ↑ | ↑ |
SNU-1 | 1.0 | 48 | 65.7 | ↑13.9 ± 0.9 | ↑30.3 ± 1.1 | ↓21.0 ± 1.4 | ↓9.3 ± 1.5 | WT | ↑ | ↑ | ↑ | UC | ↑ | UC |
KATO-III | 1.0 | 48 | 44.8 | ↑8.6 ± 0.3 | ↑27.3 ± 0.3 | ↓29.4 ± 0.2 | ↑2.1 ± 0.3 | Null | ND | ND | ND | UC | ↑ | UC |
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Ke, X.; Qin, Q.; Deng, T.; Liao, Y.; Gao, S.-J. Heterogeneous Responses of Gastric Cancer Cell Lines to Tenovin-6 and Synergistic Effect with Chloroquine. Cancers 2020, 12, 365. https://doi.org/10.3390/cancers12020365
Ke X, Qin Q, Deng T, Liao Y, Gao S-J. Heterogeneous Responses of Gastric Cancer Cell Lines to Tenovin-6 and Synergistic Effect with Chloroquine. Cancers. 2020; 12(2):365. https://doi.org/10.3390/cancers12020365
Chicago/Turabian StyleKe, Xiangyu, Qingsong Qin, Tianyi Deng, Yueyan Liao, and Shou-Jiang Gao. 2020. "Heterogeneous Responses of Gastric Cancer Cell Lines to Tenovin-6 and Synergistic Effect with Chloroquine" Cancers 12, no. 2: 365. https://doi.org/10.3390/cancers12020365