LINC00261 Is Differentially Expressed in Pancreatic Cancer Subtypes and Regulates a Pro-Epithelial Cell Identity
Abstract
:1. Introduction
2. Results
2.1. LINC00261 is Downregulated in PDAC and its Expression Correlates with PDAC Subtypes, Stage, Grade, and Patient Survival
2.2. LINC00261 is Associated with EMT and is Regulated by its Genomic Neighbour FOXA2
2.3. LINC00261 is Downregulated by TGFβ in TGFβ-Responsive Cell Lines
2.4. Downregulation of LINC00261 Expression with Two Different CRISPR-Based Systems Leads to EMT
2.5. E-Cadherin Expression is Significantly Downregulated in LINC00261low Cells
2.6. LINC00261 Downregulation Leads to Increased Cell Migration and Invasion
3. Discussion
4. Materials and Methods
4.1. Bioinformatics Analysis of LINC00261 Expression in PDAC samples
4.2. RNA Extraction from PDAC and Normal Pancreas Tissue Samples
4.3. Cell Culture and siRNA Transfection
4.4. Genomic DNA and Total RNA Extraction Followed by qRT-PCR
4.5. Protein Extraction and Western Blot Analysis
4.6. Subcellular Fractionation
4.7. Chromatin Immunoprecipitation
4.8. Cloning
4.9. LINC00261 Downregulation by CRISPR Interference
4.10. LINC00261 Promoter Knockout Using CRISPR/Cas9
4.11. RNA-Seq and Data Analysis
4.12. Luciferase Assay
4.13. 2D Cell Proliferation Assay
4.14. Cell Migration and Invasion Assays
4.15. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Dorn, A.; Glaß, M.; Neu, C.T.; Heydel, B.; Hüttelmaier, S.; Gutschner, T.; Haemmerle, M. LINC00261 Is Differentially Expressed in Pancreatic Cancer Subtypes and Regulates a Pro-Epithelial Cell Identity. Cancers 2020, 12, 1227. https://doi.org/10.3390/cancers12051227
Dorn A, Glaß M, Neu CT, Heydel B, Hüttelmaier S, Gutschner T, Haemmerle M. LINC00261 Is Differentially Expressed in Pancreatic Cancer Subtypes and Regulates a Pro-Epithelial Cell Identity. Cancers. 2020; 12(5):1227. https://doi.org/10.3390/cancers12051227
Chicago/Turabian StyleDorn, Agnes, Markus Glaß, Carolin T. Neu, Beate Heydel, Stefan Hüttelmaier, Tony Gutschner, and Monika Haemmerle. 2020. "LINC00261 Is Differentially Expressed in Pancreatic Cancer Subtypes and Regulates a Pro-Epithelial Cell Identity" Cancers 12, no. 5: 1227. https://doi.org/10.3390/cancers12051227