Rac1 Promotes Cell Motility by Controlling Cell Mechanics in Human Glioblastoma
Abstract
:1. Introduction
2. Results
2.1. Rac1 Is Essential for GBM Motility
2.2. Rac1 Signaling Regulates Myosin IIa Localization
2.3. Rac1 Signaling in Cell Adhesion Formation
2.4. Rac1 Activates Erk to Mediate Cell Adhesion Dynamics
2.5. Characterization of the Mechanical Properties in Response to Rac1 Inhibition
3. Discussion
4. Materials and Methods
4.1. Cell Lines
4.2. Gene Silencing
4.3. Immunofluorescent Staining and RT-PCR
4.4. Cell Proliferation Assay
4.5. Cell Transfection and Live Cell Imaging
4.6. Tracking Assays and Data Analysis
4.7. Active Rac1 Pull-Down Assay and Western Blot
4.8. Transwell Assay
4.9. Force Spectroscopy of Living Cells and Data Analysis
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Xu, J.; Galvanetto, N.; Nie, J.; Yang, Y.; Torre, V. Rac1 Promotes Cell Motility by Controlling Cell Mechanics in Human Glioblastoma. Cancers 2020, 12, 1667. https://doi.org/10.3390/cancers12061667
Xu J, Galvanetto N, Nie J, Yang Y, Torre V. Rac1 Promotes Cell Motility by Controlling Cell Mechanics in Human Glioblastoma. Cancers. 2020; 12(6):1667. https://doi.org/10.3390/cancers12061667
Chicago/Turabian StyleXu, Jing, Nicola Galvanetto, Jihua Nie, Yili Yang, and Vincent Torre. 2020. "Rac1 Promotes Cell Motility by Controlling Cell Mechanics in Human Glioblastoma" Cancers 12, no. 6: 1667. https://doi.org/10.3390/cancers12061667