Possible Role of IRS-4 in the Origin of Multifocal Hepatocellular Carcinoma
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Patients
2.3. Cell Culture
2.4. Immunohistochemistry (IHC) and Immunocytochemistry (ICC)
2.5. Biochemical and Molecular Biology Methods
2.6. Cellular Adhesion to Collagen Experiments
2.7. Cell Migration Assay
2.8. Statistical Analysis
3. Results
3.1. Localization of IRS-4 in Human HCC
3.2. Role of IRS-4 in the Proliferation of HepG2 Cells
3.3. Study of the Role of IRS-4 Depletion on Cell Morphology, Integrin α2 Distribution, Cell Adhesion, and Migration of HepG2 Cells
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Guijarro, L.G.; Sanmartin-Salinas, P.; Pérez-Cuevas, E.; Toledo-Lobo, M.V.; Monserrat, J.; Zoullas, S.; Sáez, M.A.; Álvarez-Mon, M.A.; Bujan, J.; Noguerales-Fraguas, F.; et al. Possible Role of IRS-4 in the Origin of Multifocal Hepatocellular Carcinoma. Cancers 2021, 13, 2560. https://doi.org/10.3390/cancers13112560
Guijarro LG, Sanmartin-Salinas P, Pérez-Cuevas E, Toledo-Lobo MV, Monserrat J, Zoullas S, Sáez MA, Álvarez-Mon MA, Bujan J, Noguerales-Fraguas F, et al. Possible Role of IRS-4 in the Origin of Multifocal Hepatocellular Carcinoma. Cancers. 2021; 13(11):2560. https://doi.org/10.3390/cancers13112560
Chicago/Turabian StyleGuijarro, Luis G., Patricia Sanmartin-Salinas, Eva Pérez-Cuevas, M. Val Toledo-Lobo, Jorge Monserrat, Sofía Zoullas, Miguel A. Sáez, Miguel A. Álvarez-Mon, Julia Bujan, Fernando Noguerales-Fraguas, and et al. 2021. "Possible Role of IRS-4 in the Origin of Multifocal Hepatocellular Carcinoma" Cancers 13, no. 11: 2560. https://doi.org/10.3390/cancers13112560