Exercise Inhibits Doxorubicin-Induced Damage to Cardiac Vessels and Activation of Hippo/YAP-Mediated Apoptosis
Abstract
:Simple Summary
Abstract
1. Introduction
2. Methods
2.1. Mice
2.2. Cell Culture
2.3. Bone Marrow Transplant (BMT)
2.4. Exercise (Ex) and Doxorubicin (Dox) Treatment
2.5. Echocardiographic Imaging
2.6. Immunohistochemical Analysis
2.7. Confocal Laser Scanning Microscope
2.8. MTT Assay
2.9. Western Blot Analysis
2.10. Statistical Analysis
3. Results
3.1. Exercise Prevents Dox-Induced Decreases in Cardiac Function and Blood Flow
3.2. Exercise Inhibits Dox-Induced Cardiomyocyte Apoptosis
3.3. Exercise Promotes Migration of Bone Marrow (Stem) Cells into the Heart after Dox Treatment
3.4. Exercise Promotes Differentiation of BM Stem Cells into Endothelial Cells and Pericytes after Dox Treatment
3.5. Effect of Dox and Dox+Ex on Cardiac Fibroblast Proliferation
3.6. Dox-Induced Cardiomyocyte Apoptosis is Mediated by Activation of Hippo-YAP Signaling
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Tao, R.-H.; Kobayashi, M.; Yang, Y.; Kleinerman, E.S. Exercise Inhibits Doxorubicin-Induced Damage to Cardiac Vessels and Activation of Hippo/YAP-Mediated Apoptosis. Cancers 2021, 13, 2740. https://doi.org/10.3390/cancers13112740
Tao R-H, Kobayashi M, Yang Y, Kleinerman ES. Exercise Inhibits Doxorubicin-Induced Damage to Cardiac Vessels and Activation of Hippo/YAP-Mediated Apoptosis. Cancers. 2021; 13(11):2740. https://doi.org/10.3390/cancers13112740
Chicago/Turabian StyleTao, Rong-Hua, Masato Kobayashi, Yuanzheng Yang, and Eugenie S. Kleinerman. 2021. "Exercise Inhibits Doxorubicin-Induced Damage to Cardiac Vessels and Activation of Hippo/YAP-Mediated Apoptosis" Cancers 13, no. 11: 2740. https://doi.org/10.3390/cancers13112740