Urine LOX-1 and Volatilome as Promising Tools towards the Early Detection of Renal Cancer
, Sabina Pucci 1,†, Manuela Costantini 3,†, Rosamaria Capuano 4, Chiara Greggi 5, Chiara Polidoro 1, Giuseppina Somma 1, Valentina Pasqualetti 4, Yolande Ketchanji Mougang 4, Alexandro Catini 4, Giuseppe Simone 3, Roberto Paolesse 6, Augusto Orlandi 1, Alessandro Mauriello 7, Mario Roselli 2, Andrea Magrini 1, Giuseppe Novelli 1,8, Corrado Di Natale 4,* and Federica Carla Sangiuolo 1,*Round 1
Reviewer 1 Report
The manuscript shows a study of LOX-1 expression in a short series of CCRCC (n=40). Although short, the series is robust and the approach appropriate. Results are of potential interest to be applied in the clinical practice. For these reasond, the paper should be accepted: I found some minor comments:
- The Introduction section is too large, and much of the information given does not focus on the topic. I would suggest to shorten it avoiding unnecessary data non-related specifically with the objective.
- Remove type I and II when speaking about papillary renal cell carcinoma, since this subclassification is no longer accepted
- Keep terminology more correct when mentioning tumor subtypes in the introduction: papillary renal cell carcinoma and chromophobe renbal cell carcinoma.
- Spell correctly Hematoxylin and Immunohistochemistry throughout the figure legends
Author Response
please see the attachment
Author Response File: 
Author Response.pdf
Reviewer 2 Report
The manuscript provides important information for biomarker researchers. I suggest to address the following comments in a revised version of the manuscript.
1. Please include a Table providing the clinic pathological parameters of the study cohort.
2. Pleas add a Section on statistical analysis in the Methods section.
3. Please perform a statistical analysis of LOX-1 expression in renal tissues.
4. Did the author study corresponding volatile compounds, urine and tissue samples? Did they observe a correlation of LOX-1 expression levels in the different samples?
5. Please add ROC analyses for tissue/urine studies.
6. For validation purposes, I suggest to include an analysis of the TCGA ccRCC dataset.
Author Response
please see the attachment
Author Response File: Author Response.pdf
Round 2
Reviewer 2 Report
The reviewer comments have been addressed, however, I recommend including the findings from the TCGA analysis into the manuscript before the manuscript should be accepted for publication
Author Response
Thank you for your suggestion.
As requested, the findings from the TCGA analysis was included into the manuscript.
best regards
