Knockdown of microRNA-214-3p Promotes Tumor Growth and Epithelial-Mesenchymal Transition in Prostate Cancer
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Cell Lines and Cell Culture
2.2. Establishment of miR-214 Knockout Cells
2.3. miR-214 and Negative Control (NC) Mimic Transfection
2.4. MTT Assay
2.5. Clonogenic Assay
2.6. Anoikis Assay
2.7. RNA Isolation and Quantification
2.8. Wound Healing Migration Assay
2.9. Migration and Invasion Assay
2.10. Western Blot Analysis
2.11. Immunofluorescence and Phalloidin Staining
2.12. RNA Isolation, Library Preparation, and RNA-Seq
2.13. In Vivo Tumorigenesis Assay
2.14. Immunohistochemical (IHC) Staining
2.15. Statistical Analysis
3. Results
3.1. Effects of miR-214 Gene Knockdown on Cell Proliferation and Colony Formation in Prostate Cancer Cells
3.2. miR-214 Knockdown Increases Anoikis Resistance, Invasiveness, and EMT in PCa Cells
3.3. Differential Expression of mRNAs in PCa Cells with miR-214 Knockdown
3.4. Knockdown of miR-214 Enhanced PCa Tumor Growth, Angiogenesis, and EMT in Tumor Xenografts
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Cagle, P.; Smith, N.; Adekoya, T.O.; Li, Y.; Kim, S.; Rios-Colon, L.; Deep, G.; Niture, S.; Albanese, C.; Suy, S.; et al. Knockdown of microRNA-214-3p Promotes Tumor Growth and Epithelial-Mesenchymal Transition in Prostate Cancer. Cancers 2021, 13, 5875. https://doi.org/10.3390/cancers13235875
Cagle P, Smith N, Adekoya TO, Li Y, Kim S, Rios-Colon L, Deep G, Niture S, Albanese C, Suy S, et al. Knockdown of microRNA-214-3p Promotes Tumor Growth and Epithelial-Mesenchymal Transition in Prostate Cancer. Cancers. 2021; 13(23):5875. https://doi.org/10.3390/cancers13235875
Chicago/Turabian StyleCagle, Patrice, Nikia Smith, Timothy O. Adekoya, Yahui Li, Susy Kim, Leslimar Rios-Colon, Gagan Deep, Suryakant Niture, Christopher Albanese, Simeng Suy, and et al. 2021. "Knockdown of microRNA-214-3p Promotes Tumor Growth and Epithelial-Mesenchymal Transition in Prostate Cancer" Cancers 13, no. 23: 5875. https://doi.org/10.3390/cancers13235875