Spontaneous Physical Activity in Obese Condition Favours Antitumour Immunity Leading to Decreased Tumour Growth in a Syngeneic Mouse Model of Carcinogenesis
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animal Model
2.2. Body and Metabolic Mice Follow-Up
2.3. Mammary Adenocarcinoma Cell Line and Fat Pad Implantation
2.4. Sacrifice and Tissue Collection
2.5. Immune Cell Phenotyping by Flow Cytometry
2.6. Frozen Tissues and Plasma Analysis
2.6.1. Tissue Preparation
2.6.2. Quantification of Biomarkers
2.7. Total Tumour RNA Isolation and Real-Time PCR
2.8. Statistical Analysis
3. Results
3.1. Enriched Environment Enhanced Spontaneous Physical Activity and Myokin Secretions
3.2. Physical Activity Influences the Secretions of the Whole Body
3.3. Enriched Environment Slowed Down Tumour Growth without an Impact on Body Weight
3.4. Enriched Environment Modified the Tumour Immune Microenvironment
3.5. Enriched Environment Modified Immune Cell Maturation but Not Differenciation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Samples | Cytokines (pg/mg) | Standard Environment | Enriched Environment |
---|---|---|---|
Plasma | IL-15 | 226.5 ± 49.4 a | 1.00 × 10−4 ± 1.00 × 10−4 b |
LIF | 6.36 ± 2.47 a | 1.00 × 10−4 ± 1.00 × 10−4 b | |
CX3CL1 | 1448 ± 404 | 1081 ± 253 | |
Tumour | IL-15 | 0.28 ± 0.19 a | 1.00 × 10−4 ± 1.00 × 10−4 b |
LIF | 0.63 ± 0.17 | 0.67 ± 0.15 | |
CX3CL1 | 0.78 ± 0.04 a | 0.55 ± 0.03 b | |
Contralateral mammary gland | IL-15 | 0.17 ± 0.17 a | 1.00 × 10−4 ± 1.00 × 10−4 b |
LIF | 0.002 ± 0.001 a | 1.00 × 10−4 ± 1.00 × 10−4 b | |
CX3CL1 | 0.42 ± 0.12 a | 0.34 ± 0.05 b | |
Gastrocnemius | IL-15 | 1.294 ± 0.25 a | 0.32 ± 0.64 b |
LIF | 6.6× 10−3 ± 1.2 × 10−3 a | 1.00 × 10−4 ± 1.00 × 10−4 b | |
CX3CL1 | 0.66 ± 0.04 a | 0.42 ± 0.14 b | |
Inguinal adipose tissue | IL-15 | 0.39 ± 0.15 a | 1.00 × 10−4 ± 1.00 × 10−4 b |
LIF | 0.020 ± 0.015 a | 1.00 × 10−4 ± 1.00 × 10−4 b | |
CX3CL1 | 0.82 ± 0.22 a | 0.43 ± 0.11 b |
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Le Guennec, D.; Goepp, M.; Farges, M.-C.; Rougé, S.; Vasson, M.-P.; Caldefie-Chezet, F.; Rossary, A. Spontaneous Physical Activity in Obese Condition Favours Antitumour Immunity Leading to Decreased Tumour Growth in a Syngeneic Mouse Model of Carcinogenesis. Cancers 2022, 14, 59. https://doi.org/10.3390/cancers14010059
Le Guennec D, Goepp M, Farges M-C, Rougé S, Vasson M-P, Caldefie-Chezet F, Rossary A. Spontaneous Physical Activity in Obese Condition Favours Antitumour Immunity Leading to Decreased Tumour Growth in a Syngeneic Mouse Model of Carcinogenesis. Cancers. 2022; 14(1):59. https://doi.org/10.3390/cancers14010059
Chicago/Turabian StyleLe Guennec, Delphine, Marie Goepp, Marie-Chantal Farges, Stéphanie Rougé, Marie-Paule Vasson, Florence Caldefie-Chezet, and Adrien Rossary. 2022. "Spontaneous Physical Activity in Obese Condition Favours Antitumour Immunity Leading to Decreased Tumour Growth in a Syngeneic Mouse Model of Carcinogenesis" Cancers 14, no. 1: 59. https://doi.org/10.3390/cancers14010059