Deciphering Genetic Alterations of Hairy Cell Leukemia and Hairy Cell Leukemia-like Disorders in 98 Patients
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Patients and Samples
2.2. Next Generation Sequencing (NGS)
2.3. Statistical Analysis and Data Representation
3. Results
3.1. Characteristics of the 98 Patients
3.2. cHCL Has a Specific Molecular Signature
3.3. Sub-Clonal Mutations and Variant Allele Frequency Changes Were Found at Relapse
3.4. KLF2 Is a Gene of Interest
3.5. Genomic Signature in HCL-like Patients
3.6. Clinical Impact and Prognosis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Variate | HCL (n = 82) | HCL-like Disorders (n = 16) | |
---|---|---|---|
vHCL/SDRPL (n = 5) | HCL-like NOS (n = 11) | ||
CLINICAL FEATURES | |||
Sex ratio (M/F) | 2.9 | 5 | 2.7 |
Median age (years [min-max]) | 55.8 [30–82] | 72 [58–82] | 74 [67–95] |
Lymphocytosis (>4 G/L) | 12/61 | 3/4 | 5/10 |
Monocytopenia (<0.2) | 41/56 | 0/4 | 2/10 |
Diagnosis (n, %), relapse (n, %) sample | 51 (63%), 30 (27%) | 4 (80%), 1 (20%) | 9 (82%), 2 (18%) |
Median number of treatment lines[min-max] | 2 [1–12] | 1 [0–1] | 0.5 [0–1] |
BM (n, %), PB (n, %) | 28 (34%), 54 (66%) | 1(20%), 4 (80%) | 1 (9%), 10 (91%) |
IMMUNOPHENOTYPE | |||
CD11c | 72/72 | 5/5 | 10/11 |
CD25 (n) | 72/72 | 1/5 | 2/11 |
CD103 (n) | 71/72 | 4/5 | 4/11 |
CD123 (n) | 65/68 | 1/4 | 2/11 |
GENETIC ALTERATIONS | |||
IGHV mutated (n, %) | 30/34 (88%) | 2/5 (40%) | 9/10 (90%) |
IGHV repertory VH4-34 (n) | 3/34 | 3/5 | 1/10 |
IGHV repertory VH3 (n) | 15/34 | 0/5 | 5/10 |
IGHV repertory VH1-2 (n) | 2/34 | 0 | 2/10 |
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Maitre, E.; Tomowiak, C.; Lebecque, B.; Bijou, F.; Benabed, K.; Naguib, D.; Kerneves, P.; Cornet, E.; Viailly, P.-J.; Arsham, J.; et al. Deciphering Genetic Alterations of Hairy Cell Leukemia and Hairy Cell Leukemia-like Disorders in 98 Patients. Cancers 2022, 14, 1904. https://doi.org/10.3390/cancers14081904
Maitre E, Tomowiak C, Lebecque B, Bijou F, Benabed K, Naguib D, Kerneves P, Cornet E, Viailly P-J, Arsham J, et al. Deciphering Genetic Alterations of Hairy Cell Leukemia and Hairy Cell Leukemia-like Disorders in 98 Patients. Cancers. 2022; 14(8):1904. https://doi.org/10.3390/cancers14081904
Chicago/Turabian StyleMaitre, Elsa, Cécile Tomowiak, Benjamin Lebecque, Fontanet Bijou, Khaled Benabed, Dina Naguib, Pauline Kerneves, Edouard Cornet, Pierre-Julien Viailly, Jeffrey Arsham, and et al. 2022. "Deciphering Genetic Alterations of Hairy Cell Leukemia and Hairy Cell Leukemia-like Disorders in 98 Patients" Cancers 14, no. 8: 1904. https://doi.org/10.3390/cancers14081904