Vitamin D3 Upregulated Protein 1 Deficiency Promotes Azoxymethane/Dextran Sulfate Sodium-Induced Colorectal Carcinogenesis in Mice
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Reagents and Animals
2.2. AOM/DSS-Induced Colitis
2.3. RNA Isolation and Quantification of mRNA Expression
2.4. Histology, Immunohistochemistry (IHC), and TdT-Mediated dUTP Nick-End Labeling (TUNEL) Assay
2.5. Western Immunoblotting Analysis
2.6. Statistical Analyses
3. Results
3.1. VDUP1 Expression Is Downregulated and Correlates with Poor Prognosis in CAC
3.2. VDUP1 Reduces Disease Severity in the AOM/DSS-Induced CAC Model Mice
3.3. VDUP1 Protects against AOM/DSS-Mediated Carcinogenesis
3.4. VDUP1 Deficiency Induces Cell Proliferation and Inhibits Apoptosis in AOM/DSS-Induced CAC Model Mice
3.5. VDUP1 Deficiency Induces the Molecular Patterns of Carcinogenesis in AOM/DSS-Induced CAC Model Mice
3.6. VDUP1 Deficiency Induces the Activation of STAT3 and NF-κB in AOM/DSS-Induced CAC Model Mice
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Park, K.H.; Kim, H.-C.; Won, Y.-S.; Yoon, W.K.; Choi, I.; Han, S.-B.; Kang, J.S. Vitamin D3 Upregulated Protein 1 Deficiency Promotes Azoxymethane/Dextran Sulfate Sodium-Induced Colorectal Carcinogenesis in Mice. Cancers 2024, 16, 2934. https://doi.org/10.3390/cancers16172934
Park KH, Kim H-C, Won Y-S, Yoon WK, Choi I, Han S-B, Kang JS. Vitamin D3 Upregulated Protein 1 Deficiency Promotes Azoxymethane/Dextran Sulfate Sodium-Induced Colorectal Carcinogenesis in Mice. Cancers. 2024; 16(17):2934. https://doi.org/10.3390/cancers16172934
Chicago/Turabian StylePark, Ki Hwan, Hyoung-Chin Kim, Young-Suk Won, Won Kee Yoon, Inpyo Choi, Sang-Bae Han, and Jong Soon Kang. 2024. "Vitamin D3 Upregulated Protein 1 Deficiency Promotes Azoxymethane/Dextran Sulfate Sodium-Induced Colorectal Carcinogenesis in Mice" Cancers 16, no. 17: 2934. https://doi.org/10.3390/cancers16172934