Composite Mineralized Collagen/Polycaprolactone Scaffold-Loaded Microsphere System with Dual Osteogenesis and Antibacterial Functions
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Preparation
2.2.1. PLGA(BMP-2) Microspheres
2.2.2. PLGA(BMP-2)/CS(Pac-525) Composite Microspheres
2.2.3. PLGA(BMP-2)/CS(Pac-525)@MC/PCL Scaffolds
2.3. Characterization of the Physical and Chemical Properties
2.3.1. Morphology
2.3.2. Encapsulation Rates of Microspheres
2.3.3. Porosity of PLGA(BMP-2)/CS(Pac-525)@MC/PCL Scaffolds
2.3.4. Compressive Strength of PLGA(BMP-2)/CS(Pac-525)@MC/PCL Scaffolds
2.3.5. Drug Release Test in Vitro
2.4. Biological Properties of PLGA(BMP-2)/CS(Pac-525)@MC/PCL Scaffolds
2.4.1. Cell Culture
2.4.2. Cell Adhesion
2.4.3. Cell Viability
2.4.4. Detection of Osteogenic Activity
2.5. Antibacterial Property of PLGA(BMP-2)/CS(Pac-525)@MC/PCL Scaffolds
2.6. Statistical Analysis
3. Results and Discussion
3.1. Morphology of Microspheres and Scaffolds
3.2. Physical and Chemical Characteristics
3.3. The Drug Release Behaviour in Vitro
3.4. Biocompatibility
3.5. Osteogenic Activity
3.6. Antimicrobial Activity
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Name | Mean Diameter (µm) | Encapsulation Ratio (%) |
---|---|---|
PLGA(BMP-2) | 8.9 ± 1.0 | 83.75 ± 3.25 |
PLGA/CS(Pac-525) | -- | 66.21 ± 2.29 |
PLGA(BMP-2)/CS(Pac-525) | 61.3 ± 15.3 | -- |
Name | Average Pore Size (µm) | Porosity (%) | Compressive Strength (Mpa) |
---|---|---|---|
PLGA(BMP-2)/CS(Pac-525)@MC/PCL | 97.7 ± 3.4 | 72.75 ± 2.61 | 0.42 ± 0.02 |
Cumulative Release Rate (%) | Microsphere | Scaffold | ||
---|---|---|---|---|
PLGA(BMP-2)/CS | PLGA/CS(Pac-525) | PLGA(BMP-2)/CS@MC/PCL | PLGA/CS(Pac-525)@MC/PCL | |
BMP-2 | 16.26 ± 0.84 | -- | 9.45 ± 1.11 | -- |
Pac-525 | -- | 66.85 ± 1.04 | -- | 59.42 ± 1.46 |
Name | PBS | 10 Days # | 20 Days # | 30 Days # |
---|---|---|---|---|
E. coli | 1 ± 0.02 | 1.57 ± 0.10 | 1.36 ± 0.04 | 1.30 ± 0.04 |
S. aureus | 1 ± 0.02 | 1.35 ± 0.01 | 1.14 ± 0.02 | 1.15 ± 0.03 |
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He, Y.; Wang, Q.; Liu, Y.; Zhang, Z.; Cao, Z.; Wang, S.; Ying, X.; Ma, G.; Wang, X.; Liu, H. Composite Mineralized Collagen/Polycaprolactone Scaffold-Loaded Microsphere System with Dual Osteogenesis and Antibacterial Functions. Polymers 2024, 16, 2394. https://doi.org/10.3390/polym16172394
He Y, Wang Q, Liu Y, Zhang Z, Cao Z, Wang S, Ying X, Ma G, Wang X, Liu H. Composite Mineralized Collagen/Polycaprolactone Scaffold-Loaded Microsphere System with Dual Osteogenesis and Antibacterial Functions. Polymers. 2024; 16(17):2394. https://doi.org/10.3390/polym16172394
Chicago/Turabian StyleHe, Yuzhu, Qindong Wang, Yuqi Liu, Zijiao Zhang, Zheng Cao, Shuo Wang, Xiaoxia Ying, Guowu Ma, Xiumei Wang, and Huiying Liu. 2024. "Composite Mineralized Collagen/Polycaprolactone Scaffold-Loaded Microsphere System with Dual Osteogenesis and Antibacterial Functions" Polymers 16, no. 17: 2394. https://doi.org/10.3390/polym16172394