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15 February 2022

Outcomes of Pulsed Electric Fields and Nonthermal Plasma Treatments on Seed Germination and Protein Functions

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1
Center of Plasma Nano-Interface Engineering, Kyushu University, Fukuoka 819-0395, Japan
2
Graduate School of Information Science and Electrical Engineering, Kyushu University, Fukuoka 819-0395, Japan
3
Faculty of Information Science and Electrical Engineering, Kyushu University, Fukuoka 819-0395, Japan
4
Center for Novel Science Initiatives, National Institute of Natural Science, Tokyo 105-0001, Japan
Agronomy2022, 12(2), 482;https://doi.org/10.3390/agronomy12020482
This article belongs to the Special Issue Applied High-Voltage Plasma Technologies in Agricultural Industry
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Abstract

To meet the needs of the hungry population, it is critical to boost agricultural product production while minimizing contaminated waste. The use of two nonthermal technologies, pulsed electric field (PEF) and nonthermal plasma (NTP), is increasing every day. As both PEF and NTP are relatively newer areas, there is limited knowledge about these two technologies and their modes of action. Studies showed that PEF treatment on the plant seeds helps germination and seedling growth. The positive impact of PEF intensity is highly dependent on the seed coat type and plant species. Another nonthermal technology, NTP, affects seed germination, seedling growth, yield, and resilience to abiotic stress when generated at varying pressures with and without different feed gases. Early germination, germination rate, and germination percentage were all improved when the seedlings were treated with NTP. Similarly to the PEF treatment, NTP had a negative or no effect on germination. This review examined the effects of PEF and NTP on seed germination and analyzed the situation and mechanism behind the positive or negative effect. Deactivation of proteins and enzymes to extend the shelf life of beverages is another prominent application of PEF and NTP. The interaction of PEF and NTP with proteins aids in understanding the microscopic mechanism of these technologies. Therefore, we covered in this review the potential structural and functional changes in proteins/enzymes as a result of PEF and NTP, as well as a comparison of the benefits and drawbacks of these two technologies.

1. Introduction

The electric field affects plants in both soil and air, as the Earth has an electrified environment maintained by a global circuit. Plant cells contain membrane potential because of ion channels or pumps that allow electric current to flow through them [1]. Various studies have been conducted to study the electric current effect on plants [2]. According to Kotaka et al., the action of ions (positive or negative) in the air can affect variations in plant respiration [3]. Song et al. [4] and Lee et al. [5] showed the impact of air anions on the growth of lettuce and kale, respectively. Seed germination starts with water uptake by the seed and ends with embryonic axis extension. This can take 24–36 h depending on the germination conditions, followed by radicle elongation [6]. Frequently, plants are facing multiple stress that results in decreasing crop yields. Different types of seed treatment techniques were used to increase crop yield under regularly changing environmental stress [7].
Traditional methods, such as cooking, boiling, frying, drying, etc., are frequently used to increase the shelf life of food products [8]. As the world population increases, there is a drastic increase in the consumption of processed foodstuffs that increases chemical additives and thermal processing. This results in inadvertent outcomes such as the formation of toxic compounds, nutrient losses, adverse effects on color and texture, etc. [9]. Nonthermal technologies such as pulsed electric field (PEF) and nonthermal plasma (NTP) have been used to solve these problems.
PEF has shown its importance in the food industry by assisting in different processes such as improvement in oil and juice extraction yields [10,11], secondary metabolites’ biosynthesis stimulation [12,13], cold pasteurization [14], improvement of nutritional and functional properties of liquid foods [15], seed germination [16], inactivating microorganisms [17], nutrient solution for hydroponic system [18], etc. In general, high-voltage pulses are placed between two electrodes for a brief time; depending upon the voltage supplied, the PEF is categorized into low/moderate-intensity PEF (10–2000 kV/m) and high-intensity PEF (2000–4500 kV/m) [19]. PEF works on the electrical breakdown and electroporation process (formation of holes in the cell membrane and phospholipid bilayers) [15,20]. PEF treatment can result in an interruption in the plant cell wall that can help in improving the extraction process of target compounds [21]. Additionally, the applied electric field can modify the electrical sensitive components and accelerate heat and mass transfer in drying and freezing processes [10]. PEF treatment also alters the proteins’/enzymes’ structure [20] and the polarization of molecules with dipole moments (changes can be reversible or irreversible depending upon the molecules and treatment conditions) [15]. Additionally, the effectiveness of PEF treatment depends upon the food matrix (pH, composition, electrical conductivity) and operating parameters (electric field intensity, pulse polarity, pulse shape, treatment time, and frequency) [22].
Nonthermal plasma (NTP) is a complicated combination of electrons, positive and radicals, negatively charged ions, neutral atoms, and molecules [23]. NTP can produce both ROS and reactive nitrogen species (RNS), and their concentration depends upon the feed gas and plasma parameters. Depending on the concentration of reactive species and the nature of biological species, they have positive and negative impacts on biological systems. The utilization of plasma in agriculture has increased promptly in both pre-harvest and post-harvest cases [7,24,25]. Our group recently increased soil fertility by producing NH4NO3 using NTP that helped in increased germination percentage and yield of radish sprouts [26]. NTP treatments such as direct or indirect treatment showed bactericidal effects through protein oxidation, DNA and cell membrane damage, etc. [27,28,29]. In contrast, the NTP treatment could activate the plant growth-promoting bacteria [30]. NTP properties make it an ideal technology to treat beverages to increase their shelf life [31].
This review focused on the germination rate/percentage and structural and functional changes in proteins/enzymes after PEF and NTP treatments to elaborate their roles in seed treatment and beverages’ preservation.

2. Germination Rate and Seedling Growth in Plants after the Pulsed Electric Field Treatment

There are limited reports on the impact of PEF on seed germination and seedling growth, as shown in Table 1. Through this review, we focused on some of the reported work by the various groups on the diverse plant species such as Leaf Lettuce, Barley, Arabidopsis, Kale, Wheat, Chickpea, Mung bean, Bitter gourd, Tomato, Medicago Sativa, Chili, Smallflower Morningglory, and Green Foxtail.
Table 1. Changes in the growth parameters of plants after PEF treatment.
Wheat seeds treated with PEF at 140 kV/m (100 pulses) [34] and 600 kV/m (50 pulses) [35] results in increased seedling growth. PEF treatment at 200 kV/m (100 pulses) decreases coleoptile and primary leaf growth [34]. The increase in glutathione content and antioxidant enzyme activity was observed at 200 kV/m (100 pulses), while no significant change was observed at 140-kV/m (100 pulses) treatment [34]; 600-kV/m (50 pulses) treatment resulted in a significant increase in soluble proteins, carotenoids, chlorophylls, total phenolic contents, etc. for treated seeds’ plantlets’ juice, compared to the untreated seeds’ plantlets’ juice [35]. This shows that low-intensity PEF with fewer pulses or high-intensity PEF with fewer pulses significantly contributed to wheat germination and seedling growth than high-intensity PEF with high pulses.
Barley seeds’ treatment with electric field 100 kV/m resulted in a significant increase in germination for the first fraction of treatment while it declined for the second fraction of the electric field [37]. In another study, barley seeds, when treated with a high-voltage electric field (3.5 and 5 kV) for 35 min continued up to 10 days, resulted in significant increase in seed germination. At the same time, no positive effect was observed for 0, 2, 6.5, 8, and 9.5 kV. This shows that medium voltage is better for seed germination, and high or low voltage is ineffective to promote germination [38]. However, barley seeds treated with 120-kV/m electric fields result in radicle elongation without affecting the gross metabolic activity and α-amylase concentration decreased for treated seeds [46]. Chickpea seeds’ treatment of ~47-kV/m PEF for 15 min caused increased mean germination time [40]. In another study, Chickpea seeds treated with an electric field (3 V and 6 V induced for 10 min for 100 days) showed the enhanced rate of seed germination, while no change was observed for 12 V, although a decrease in seedling root and shoot length was observed for 12-V treatment as compared to 3 V and 6 V treatment [41].
The above data showed that low-intensity electric field has no significant effect on seed germination, seedling growth, and biochemical effect. In contrast, high electric field intensity causes a negative impact on seed germination, but it significantly affects the biochemical changes in the plants. At the same time, the medium electric field strength is best for germination and seedling growth. However, the electric field strength value for medium and high can depend upon the seed coat thickness. The increased seedling emergence of seeds under particular PEF treatments can be attributed to hard seed coats. Seeds can absorb water when the seed coat is damaged by PEF treatment, allowing for water imbibition, whereas seeds lacking solid seed coats result in the embryo’s damage, which causes decreased seedling emergence with the same amount of PEF intensity [45]. Another possibility is that the electric field can activate the ion transport that helps in improving the nutrient uptake [16]. It is worth noting that significant changes in the biochemical properties were observed (mainly at high electric field strength) that showed no direct correlation with the germination of seeds, although the change in biochemical properties, such as protein level, antioxidant level, etc., can influence plant growth and yield. Protein/enzyme plays an essential role in plant growth, and the deactivation of enzymes can improve the shelf life and quality of beverages such as juices, which are mentioned in the following section.

3. Impact of the PEF on the Proteins’/Enzymes’ Conformation and Activity

Changes in the conformation and function of proteins/enzymes are shown in Table 2.
Table 2. Conformational and functional changes in proteins/enzymes after PEF treatment.
Soybean protein treated with a 3000-kV/m electric field increases random coils and β-sheets and decreases in α-helix [47]. The PEF treatment changes the vibrational peaks of S-S and C-C, as indicated by the disturbance in sulfhydryl’s and disulfide bonds. PEF treatments influenced the hydrophobicity of protein by changing the tyrosine vibration frequency [47]. Another study showed 3500-kV/m intensity of PEF treatment on soybean protein resulted in conversion of β-turn to α-helix and induced a change in the orientation of the α-helix dipole moment. Additionally, at high pulse intensity, there was an increase and decrease in anti-parallel β-sheets’ and β-sheets’ contents, respectively [48]. These structural changes are due to a change in the bond vibration of C-O, C-O-H, C-O-C, C-H, and N-H by PEF treatment [48]. Soybean lipoxygenase protein treated with 4200 kV/m caused maximum inactivation of 88% after 1036 μs [49]. Another group achieved the 84.5% inactivation of soybean lipoxygenase after a 4000-kV/m treatment with preheating to 50 °C after 100 μs [50]. This shows that preheating can speed up the PEF-induced enzyme deactivation rate.
Inactivation of polyphenol oxidase was stronger than peroxidase in apple juice, showing that inactivation strongly depends upon the protein structure [59]. Some studies revealed the extent of deactivation of peroxidase in tomato juice [58] and polyphenol oxidase in grape juice [60] after PEF treatment, depending upon the intensity of the electric field and treatment time. Pectin methyl esterase protein in orange juice and red grapefruit juice treated with PEF gives rise to inactivation [61,62]. Pectin methyl esterase protein in orange juice showed a maximum inactivation of about 80% when juice samples were processed at 3500 kV/m without exceeding temperature to 37.5 °C [61]. Conversely, 96.8% inactivation of pectin methyl esterase in red grapefruit juice was obtained with a 50-°C preheating and 4000-kV/m PEF treatment [62]. Hence, pectin methyl esterase inactivation increases as the electric field strength, treatment times, and preheating temperatures increase. The distortion of the α-amylase structure and activity was obtained when treated for the higher electric field (>750 kV/m). These changes are not the result of the increase in temperature by the Joule’s effect but due to modification in the tryptophan residues [63]. Another study supported the above statement as α-amylase activity decreased as pulses increased at 25 °C [64].
The above studies clearly showed that a higher electric field is required to deactivate the enzymes. Still, the extent of the deactivation depends upon the enzyme structure and treatment time while also being influenced by the preheating treatment.
NTP is another nonthermal treatment that has gained attention in seed germination and enzyme deactivation.

4. Effect of NTP on Seed Germination

NTP treatment on the plant seeds affects seed germination, seedling growth, and yield of the plant. Our previous reviews described the plasma action on seedling growth and probable mechanism [7,24]. The current study focused on the latest results on seed germination using NTP, as shown in Table 3.
Table 3. Effect of NTP on seed germination.
Recently, heat-stressed Rice (Oryza sativa) seeds treated with Air-scalar DBD plasma with 2.17-W discharge power showed a higher germination rate than without plasma heat-stressed seeds throughout the germination time course [65]. Rice seeds with low-pressure (~1333 Pa) DBD plasma working with Ar + Air gas mixture [66], hybrid cold plasma (HCP) with Ar feed gas [67], and Air-atmospheric pressure multi-pin plasma generator [68] showed an increase in seed germination and germination rate. The percentage increase in seed germination and germination rate depends on the plasma device and treatment time and it is independent of the pressure (positive effect shown in low-pressure and atmospheric pressure).
In plasma agriculture, another most-studied plant species was radish (Raphanus sativus). Radish treated with Air-scalar DBD [70], Ar-plasma jet [74], and Air-radio-frequency (RF) low-pressure (40 Pa) plasma [76] showed a positive effect in the seed germination. Our recent work reported that an increase in maximal germination percentage depends upon the harvest year and seed coat color [70]. The maximal germination percentage increased by 8% for gray-color seeds for the 2017 harvest while not influencing the brown seeds. The plasma effect had no significant effect on seed germination for the 2018 harvest for brown and gray radish seeds [70]. This might be the reason that, in other studies, growth enhancement was reported after the various plasma treatment (scalar DBD, plasma torch, and RF-low pressure (100 Pa plasma) with O2 and N2 feed gases) but not the germination rate or percentage [71,72,73,75]. RF-low pressure (100 Pa) plasma with O2 feed gas showed increased sprouts’ length, but no change was observed on seed germination [75], while RF-low pressure (40 Pa) plasma with Air feed gas showed increased seed germination rate and growth enhancement [76]. Some authors reported that radish seeds treated with O2- RF-low pressure (100 Pa) plasma showed no morphological changes on seed coat using SEM analysis and no oxides were formed (seed surface not modified) by FTIR-ATR spectra [75], while no surface analysis was performed using Air- RF-low pressure (40 Pa) plasma [76]. There might be seed surface modification that occurred at 40 Pa, or, as we discussed above, the harvest year and seed coat color also play an essential role in the germination of radish sprouts.
Barley (Hordeum vulgare) seeds’ treatment with microwave-driven Air plasma showed a negative effect on maximum germination [79], while barley seeds treated with low-pressure (26 Pa) plasma had no effect on the germination [81], whereas treatment with surface dielectric barrier discharge (SDBD) plasma resulted in the early growth of barley sprouts [80]. The increase in early growth and fresh weight of barley sprouts was due to improved levels of the primary metabolites (soluble sugars and free amino acids) and secondary metabolites (saponarin, GABA, and policosanols) [80]. Soybean (Glycine max) seeds treated with O2-diffuse coplanar surface barrier discharge (DCSBD) plasma [82] and HD-2N low-pressure (150 Pa) plasma [84] resulted in a positive effect on seed germination. The reduced germination of control soybean seeds was due to seeds’ age. The increased germination after DCSBD was due to a positive impact on succinate dehydrogenase activity, while inhibition of germination at high DCSBD doses caused by significant oxidative stress resulted in lipid peroxidation, stimulated lactate, and alcohol dehydrogenase activities and inhibited succinate dehydrogenase activity [82]. Maize seeds (Zea mays) treated with low-frequency glow discharge plasma (LFGD) at ~53,328.9 Pa [86] and Air-DCSBD caused a positive influence in germination [87]. LFGD treatment enhances enzymatic activities related to seed germination and stimulates the decomposition of the essential nutrients that trigger compounds to increase the consumption of seed reserves and promote seedling [86]. Dehydrogenase activity was significantly higher in embryos after the seed was treated with Air-DCSBD that might affect early germination [87].
We observed that wheat was the most studied plant species using NTP at low pressure and atmospheric pressure, and, in the majority of cases, it showed a positive impact on germination. Wheat (Triticum spp.) seeds treated with low-pressure plasma [88,89] revealed an improved germination rate and germination potential. Similarly, wheat (Triticum aestivum) seeds treated with surface discharge plasma [90], low-frequency glow discharge plasma at ~1333 Pa [91,93], and atmospheric pressure DBD [92] showed increased germination percentage and germination rate, while low-pressure (140 Pa) Plasmonic AR-550-M plasma [94] demonstrated inhibited germination but enhanced footstalk. Wheat (Xiaoyan 22) seeds treated with DBD plasma working with various working gases showed an increase in germination rate and germination potential [95,96,97]. Additionally, wheat (Giza 168) [98] and winter wheat [99] seeds treated with plasma jet and DBD reactor also showed enhanced germination potential, respectively.
The impact of feed gases on the wheat germination was also reported, as the moderate-intensity DBD plasma in the presence of Air, N2, and Ar plasmas showed positive impacts on the seed germination, while no significant effect was obtained for the O2 plasma treatment. This was because the etching effect was stronger for Ar and N2 plasma than O2 plasma. Additionally, the relative electroconductivity of wheat increased for Air, N2, and Ar plasmas that remained consistent with the change in water uptake and germination. Moreover, soluble protein production was also improved after plasma treatment; hence, plasma treatment can accelerate the physiological reactions that increase the germination index [95]. It was also mentioned that plasma treatment helps in penetrating active compounds, which further results in an accelerated wheat seed germination process [98]. H2O2 plays a key role in the plant life cycle, such as the release of dormancy during seed germination [110]. It was also reported that external H2O2 treatment helps in seed germination [111]. Thus, H2O2 is produced during the plasma discharge taken up by seeds during the imbibition process and regulates the metabolic processes that helps in germination [99].
Almost all the researchers found that NTP treatment assists the penetration of RONS to the seeds that benefit from increasing protein and antioxidant levels and accelerates the physiological reactions. During these processes, the reaction of RONS with proteins may have both positive and negative impacts on the plants. Additionally, NTP is used to deactivate enzymes in beverages such as fruit juices to maintain the quality of food products. To consider this, we focus on the NTP-induced modification in proteins’/enzymes’ structures and functions in the next section.

5. NTP Effect on the Structural and Functional Changes of Proteins/Enzymes at Atmospheric Pressure

Like the PEF effect on proteins/enzymes, plasma treatment also modified the proteins’/enzymes’ structure and function, as described in Table 4.
Table 4. Conformational and functional changes of proteins/enzymes after NTP treatment.
The lysozyme treated with a low-frequency (LF) plasma jet using He + O2 feed gas decreases the residual activity and alters the secondary structure. After 30 min of plasma treatment, the lysozyme’s random coil structure formed was nearly the same as that treated with 6.0 M guanidine HCl. Quenching was observed without change in maximum wavelength in fluorescence spectra, and this was due to the chemical modification of tryptophan by NTP treatment. Some authors suggested that the change in lysozyme structure was due to reactive species such as ∙OH, O2−∙, HO2∙, and NO∙ that cause modification in the side chain of the amino acids such as tryptophan, tyrosine, cysteine, and phenylalanine [113]. In another study, the lysozyme was treated with DBD and Jet for 8 min, 12 min with Air and N2 as feed gases, resulting in decreased activity and structural changes. Circular dichroism (CD), fluorescence, and thermodynamic analysis revealed that the structure of lysozyme is more disturbed with N2 feed gas plasma than Air feed gas plasma. X-crystallographic data of lysozyme after NTP treatment showed that structural changes occurred at loop 3 and loop 6 along with the substrate-binding sites Tryptophan 62 and 108 and Aspartic acid 101.
Additionally, similar to the above work, the authors observed quenching in the fluorescence intensity without changing the wavelength after the plasma treatment. More structural and functional changes were observed in N2 plasma than Air plasma because N2 plasma generates more ∙OH and H2O2 than Air plasma. The N2 metastable level (N2(A3Σu+)) can dissociate H2O to produce ·OH and ∙H. In addition, the exciting 2N atom also generates two OH radicals, while the presence of O2 in Air plasma reduced the OH density, affecting the H2O2 concentration [114]. Both studies showed that, independent of the plasma devices and feed gases, the decrease in residual activity and a change in the secondary structure was observed; no wavelength shift in the fluorescence spectra was noticed, while the quenching of the peak was observed in both studies [113,114]. Moreover, both studies concluded that the structural changes and functional changes in lysozyme were due to RONS generated by plasma, especially OH radical [113,114], rather than plasma-induced UV [113].
Peroxidase and polyphenol oxidase treated with Ar + O2 gas mixtures’ plasma Jet caused structural and functional changes. The α-helix content decreased, and β-sheet content increased after plasma treatment for both peroxidase and polyphenol oxidase proteins. In comparison, activity was reduced to 85% for peroxidase and 90% for polyphenol oxidase after plasma treatment [124]. In a separate study, horseradish peroxidase enzyme treated with Ar + O2 DBD Jet decreased to 17% after a 10-min plasma treatment. Structural changes were similar to the above study in that the α-helix content decreased and β-sheet increased [125]. Peroxidase tomato extract treated with He and Ar DBD plasma and Air gliding arc results in deceased peroxidase activity to 7.32% after plasma treatment [126]. Further, the residual activity of peroxidase and polyphenol oxidase in carrot juice treated with Air DBD plasma reduced to 15.73 and 11.20%, respectively [127]. These studies showed that peroxidase and polyphenol oxidase activity could be reduced after the NTP treatment, independent of plasma sources and feeding gases. Although peroxidase showed more resistance to NTP treatment than polyphenol oxidase in all the cases, the structural changes were similar for peroxidase and polyphenol oxidase (α-helix content decreased and β-sheet increased).

6. Conclusions

PEF and NTP have great potential in agriculture (improved seed germination, seedling growth, high yield, and stress tolerance) and food processing (inactivation of enzymes in beverages such as fruit juices). Both technologies are environmentally friendly and easy to use. Understanding the mechanism behind improved seed germination after PEF and NTP treatments, on the other hand, is critical for future technological investigation and standardization. Different degrees of electric field strength were employed on different plant seeds in these studies, making it difficult to determine the particular electric field intensity that promotes seed germination. Nonetheless, we inferred from the research that moderate PEF intensity causes seed coat degradation, which increases water imbibition and allows an embryo to stretch, which aids seed germination. In contrast, seeds treated with a high intensity of PEF results in delayed seed germination that might damage the embryo, resulting in decreased seedling emergence. Therefore, it is important to use the same electric field intensity on different plant seeds under controlled atmospheric conditions to standardize the PEF treatment that helps in promoting germination. One of the mechanisms behind the PEF treatment is the modification of biomolecule structures (secondary and tertiary) and functional properties. It was noticed that PEF-induced structural changes also alter the physicochemical and functional properties of proteins/enzymes. In general, a decrease in the α-helix content and an increase in the β-sheet were observed after moderate PEF treatment, while high-intensity PEF treatment caused aggregation. Furthermore, after PEF treatment, enzyme activity dropped, which was substantially influenced by treatment time, enzyme native structure, PEF intensity, and preheating temperature. However, more research at consistent NTP treatment circumstances and with numerous plant seed types is still needed. According to the investigations, there is no universal rule that assists seed germination in terms of pressure, power density, working gas composition, or treatment time. Furthermore, there was no clear answer to whether NTP enhanced physiological reactions by modifying the seed surface or not. In the maximum studies, no significant change in the morphology of the seed coat was reported. However, it was known that reactive species (RONS) and UV/VUS generated by NTP initiate the reactions in seeds despite the fact that PEF induced seed coat damage that helped in nutrients’ uptake.
NTP’s role in preserving fruit juices by deactivating enzymes and proteins showed both an increase and decrease in activity depending upon the treatment time and NTP source. Like PEF, the reduction in the α-helix content and increase in β-sheet were observed in most cases after NTP treatment. Additionally, it was clearly shown in the NTP treatment the reactive species produced by NTP oxidized the amino acids of the proteins. Indeed, the oxidation of amino acids depends upon the treatment conditions, reactive species’ concentration, and the oxidative property of amino acids (some are more prone to oxidation).
The overall result is that PEF and NTP treatment aid seed germination and seedling growth but also alter the structure and function of proteins and enzymes. Additionally, there is a need to develop the standard protocol using PEF or NTP parameters, treatment time, various plant seeds, seed coat color, harvest year, seed morphology, feeding gases composition, etc. to use these technologies at an industrial scale.

Author Contributions

Writing—original draft preparation, P.A.; writing—review and editing, T.O., K.K., M.S., D.W., K.T. (Katsuyuki Takahashi) and K.T. (Koichi Takaki). All authors have read and agreed to the published version of the manuscript.

Funding

This work was supported by JSPS-KAKENHI grant numbers JP20K14454, JP16H03895, JP19H05611, JP19H05462, JP19K14700, JP20H01893, and JP21H04451; JSPS: Core-to-Core Program JPJSCCA2019002; MEXT: function enhancement expenses; Plasma Bio Consortium, The Center for Low-temperature Plasma Sciences, Nagoya University.

Institutional Review Board Statement

Not applicable.

Data Availability Statement

Not applicable.

Acknowledgments

All authors contributed to the planning and writing of the paper.

Conflicts of Interest

The authors declare no conflict of interest.

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