Molecular Mechanism of Exogenous Magnesium in Regulating Cation Homeostasis in Roots of Peanut Seedlings under Salt Stress
Abstract
:1. Introduction
2. Materials and Methods
2.1. Test Material Culture
2.2. Experiment Design
2.3. Test Method
2.3.1. Determination of Physiological Phenotypic Indicators
2.3.2. Transcriptome (RNA-Seq) Sequencing
2.3.3. RT-qPCR Detection
2.4. Data Processing and Data Visualization Methods
2.4.1. Screening of Differentially Expressed Genes (DEGs)
2.4.2. GO Enrichment Analysis and KEGG Enrichment Analysis
3. Results
3.1. Exogenous Magnesium Alleviates Root Growth and Ion Homeostasis of Peanut Seedlings under Salt Stress
3.2. DEG Screening and KEGG Enrichment Analysis of DEGs
3.3. Mining Differentially Expressed Genes of Magnesium Ion in Response to Cation Homeostasis of Peanut Seedlings under Salt Stress
3.3.1. Regulation of Exogenous Mg2+ on Key Genes of Mg2+ Transport Channel and Non-Selective Ion Channel
3.3.2. Regulation of Exogenous Mg2+ on Key Genes of Ca2+ Transport Channel and Na+/Ca2+ Ion Exchange Channel
3.3.3. Regulation of Exogenous Mg2+ on Key Genes of H+ Transport Channel, Na+ Transport Channel, and Na+/H+ Ion Exchange Channel
3.3.4. Regulation of Exogenous Mg2+ on Key Genes of K+ Transport Channel
3.4. RT−qPCR Verification
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Wang, R.; Dong, X.; Gao, Y.; Hao, F.; Zhang, H.; Lin, G. Molecular Mechanism of Exogenous Magnesium in Regulating Cation Homeostasis in Roots of Peanut Seedlings under Salt Stress. Agronomy 2024, 14, 724. https://doi.org/10.3390/agronomy14040724
Wang R, Dong X, Gao Y, Hao F, Zhang H, Lin G. Molecular Mechanism of Exogenous Magnesium in Regulating Cation Homeostasis in Roots of Peanut Seedlings under Salt Stress. Agronomy. 2024; 14(4):724. https://doi.org/10.3390/agronomy14040724
Chicago/Turabian StyleWang, Rongjin, Xuan Dong, Yan Gao, Fei Hao, Hui Zhang, and Guolin Lin. 2024. "Molecular Mechanism of Exogenous Magnesium in Regulating Cation Homeostasis in Roots of Peanut Seedlings under Salt Stress" Agronomy 14, no. 4: 724. https://doi.org/10.3390/agronomy14040724