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Article

The Expression of Genes Involved in Synthesis of Bitter Acids and Xanthohumol and the Content of These Compounds in Aroma and Bitter Hop under Reduced Nitrogen Fertilisation

Department of Plant Breeding and Biotechnology, Institute of Soil Science and Plant Cultivation—State Research Institute, Czartoryskich 8, 24-100 Puławy, Poland
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Agronomy 2024, 14(8), 1680; https://doi.org/10.3390/agronomy14081680 (registering DOI)
Submission received: 3 July 2024 / Revised: 26 July 2024 / Accepted: 29 July 2024 / Published: 30 July 2024
(This article belongs to the Section Crop Breeding and Genetics)

Abstract

Hop (Humulus lupulus) is cultivated for industrial purposes, primarily for brewing, but also for pharmaceutical and cosmetic applications. The hop cones are valued for their secondary metabolites, including bitter acids and prenylflavonoids. The objective of the presented study was to investigate the impact of reducing nitrogen fertilisation on the activity of genes involved in the production of bitter acids and xanthohumol in cultivars Lubelski (aroma) and Magnat (bitter) grown in the field. Furthermore, the content of these compounds was determined. The results demonstrated that the majority of studied genes were expressed at a similar level despite the reduction in the nitrogen dose by 25–30%. Nitrogen dose influenced the activity of genes BCAT2, HS1, and PAL. Moreover, the differences in gene expression were observed between cultivars and developmental stages. The reduction in nitrogen dose did not result in a reduction in secondary metabolite content. The content of alpha and beta acids in Magnat was approximately 14.8% and 3.2%, respectively, while in Lubelski it was approximately 7.7% and 5.8%, respectively. The content of xanthohumol in both cultivars was approximately 0.7%.
Keywords: hops; Humulus lupulus; nitrogen fertilisation; gene expression; bitter acids; xanthohumol hops; Humulus lupulus; nitrogen fertilisation; gene expression; bitter acids; xanthohumol

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MDPI and ACS Style

Czubacka, A.; Skomra, U.; Agacka-Mołdoch, M.; Koziara-Ciupa, M. The Expression of Genes Involved in Synthesis of Bitter Acids and Xanthohumol and the Content of These Compounds in Aroma and Bitter Hop under Reduced Nitrogen Fertilisation. Agronomy 2024, 14, 1680. https://doi.org/10.3390/agronomy14081680

AMA Style

Czubacka A, Skomra U, Agacka-Mołdoch M, Koziara-Ciupa M. The Expression of Genes Involved in Synthesis of Bitter Acids and Xanthohumol and the Content of These Compounds in Aroma and Bitter Hop under Reduced Nitrogen Fertilisation. Agronomy. 2024; 14(8):1680. https://doi.org/10.3390/agronomy14081680

Chicago/Turabian Style

Czubacka, Anna, Urszula Skomra, Monika Agacka-Mołdoch, and Marta Koziara-Ciupa. 2024. "The Expression of Genes Involved in Synthesis of Bitter Acids and Xanthohumol and the Content of These Compounds in Aroma and Bitter Hop under Reduced Nitrogen Fertilisation" Agronomy 14, no. 8: 1680. https://doi.org/10.3390/agronomy14081680

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