Microbiome Analysis Revealed the Effects of Environmental Factors on the Presence of Toxigenic Fungi and Toxin Production in Rice Grains
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. DNA Extraction, PCR Amplification, and High-Throughput Sequencing
2.3. Illumina NovaSeq Sequencing
2.4. Determination of Nutrient Elements, Quality Index, and Mycotoxin Contents
2.5. Amplicon Sequence Processing and Analysis
2.6. Statistical Analysis
3. Results
3.1. Diversity Analysis of Fungal Community Structures
3.2. Dominant Taxonomic Groups in Rice Grains
3.3. Correlation Analysis between Fungal Distribution and the Influence Factors
3.3.1. The Impact of Intrinsic Influence Factors on Fungal Taxa
3.3.2. The Impact of Extrinsic Influence Factors on Fungal Taxa
3.4. Fungal Functional Prediction
4. Discussion
5. Conclusions
- Regional management measures. As studies have found significant differences in the diversity and composition of fungal communities in different regions, management strategies should be developed in each region according to specific ecological conditions and fungal characteristics. For example, more targeted control measures can be taken for the core plant pathogenic fungi in their respective regions.
- Strengthen field management. Optimize planting and harvesting time (e.g., timely sowing and rational harvesting) to reduce environmental conditions conducive to fungal growth. Select disease-resistant varieties suited to local climate and soil conditions to improve the crop’s ability to resist fungal infection.
- Nutrient element management. According to the effect of nutrient elements on fungal community structure mentioned in the study, rational fertilizer application and balanced soil nutrients will improve the growth and health of the crop, thus reducing the production of fungi and mycotoxins.
- Climate adaptation measures. Given that climatic conditions are important factors affecting fungal communities, adaptive agricultural measures should be taken to cope with the effects of climate change on rice growth and fungal growth. For example, selecting moisture-tolerant rice varieties to cope with changes in rainfall.
- Monitoring and testing system. Establish a long-term monitoring program to regularly assess fungal contamination and mycotoxin levels in rice from different regions and take timely countermeasures. Such monitoring can also help to better understand the dynamics of fungal communities.
- Public and farmer education. Enhance farmers’ and public awareness of fungal contamination and its potential hazards, and improve their knowledge of good agricultural practices (GAP) and food safety so as to reduce the risk of fungal contamination at the source.
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Name | Methods Description | Reference |
---|---|---|
Ca, Fe, K, Mg, Mn, Na, P, and Zn | Determination of multi-elements in food—inductively coupled plasma optical emission spectrometry (ICP-OES) | [24] |
Protein converted by N (PRO) | Determination of crude protein content of cereals and legumes—Dumas combustion method | [25] |
Fat (FAT) | Determination of fat in food—Soxhlet extractor method | [26] |
Starch (STA) | Grain crude starch determination method—polarimetry | [27] |
Reducing Sugar (RS) | Determination of reducing sugar in food—colorimetry | [28] |
Water (W) | Determination of moisture in food—direct drying | [29] |
Deoxynivalenol (DON) | Determination of deoxynivalenol and its acetylated derivatives in food | [30] |
Zearalenone (ZEN) | Determination of zearalenone in food | [31] |
Aflatoxin B group (AFT) | Determination of aflatoxin B and G in food | [32] |
Fumonisin (FB) | Determination of fumonisin in food | [33] |
Total ustiloxin (USTs) | Determination of rice false smut toxin liquid—chromatography–mass spectrometry | [34] |
Environmental Factors | CCA1 | CCA2 | r2 | p Value |
---|---|---|---|---|
SMT | −0.9997 | −0.0251 | 0.4865 | 0.001 |
Months of harvesting | 0.5316 | −0.847 | 0.4721 | 0.001 |
Months of sowing | 0.2855 | −0.9584 | 0.4292 | 0.001 |
HMP | −0.8651 | 0.5016 | 0.4211 | 0.001 |
Var1 | −0.9996 | 0.0277 | 0.4180 | 0.001 |
Var2 | 0.3524 | −0.9358 | 0.4136 | 0.001 |
Latitude | 1.0000 | −0.0053 | 0.3872 | 0.001 |
MAP | −0.9916 | −0.1296 | 0.1752 | 0.001 |
Windspeed | 0.6544 | 0.7561 | 0.0481 | 0.083 |
Altitude | −0.0380 | −0.9993 | 0.0025 | 0.890 |
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Zhang, F.; Cao, Z.; Zhao, X.; Yan, Q.; Guan, M.; Chen, M.; Lin, X. Microbiome Analysis Revealed the Effects of Environmental Factors on the Presence of Toxigenic Fungi and Toxin Production in Rice Grains. Agronomy 2024, 14, 1681. https://doi.org/10.3390/agronomy14081681
Zhang F, Cao Z, Zhao X, Yan Q, Guan M, Chen M, Lin X. Microbiome Analysis Revealed the Effects of Environmental Factors on the Presence of Toxigenic Fungi and Toxin Production in Rice Grains. Agronomy. 2024; 14(8):1681. https://doi.org/10.3390/agronomy14081681
Chicago/Turabian StyleZhang, Fengmin, Zhenzhen Cao, Xiaohua Zhao, Qing Yan, Meiyan Guan, Mingxue Chen, and Xiaoyan Lin. 2024. "Microbiome Analysis Revealed the Effects of Environmental Factors on the Presence of Toxigenic Fungi and Toxin Production in Rice Grains" Agronomy 14, no. 8: 1681. https://doi.org/10.3390/agronomy14081681