Comprehensive Dissection of Metabolites in Response to Low Phosphorus Stress in Different Root-Type Alfalfa at Seedling Stage
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Sand Culture
2.2. Stress Treatments and Sampling
2.3. Evaluation of Growth Indices
2.4. Determination of Morphological Indicators
2.5. Measurement of Phytohormones
2.6. Measurement of Organic Acids
2.7. Metabolite Extraction and Determination
2.8. Statistical Analysis
3. Results
3.1. Effects of P Stress on the Growth of Different Root-Type Alfalfa
3.2. Metabolomics Analysis
3.2.1. The Establishment of the Mass Spectrometry Analysis System
3.2.2. Principal Component Analysis
3.2.3. Differential Metabolite Screening
3.2.4. Analysis of Differentially Metabolized Substances
3.2.5. KEGG Pathway Enrichment Analysis
3.3. Effects of P Stress on Levels of Organic Acid Content
3.4. Effects of P Stress on Levels of Phytohormones
4. Discussion
4.1. Effects of LP Stress on the Growth of Different Root-Type Alfalfa
4.2. Effects of LP Stress on Differential Metabolites in Roots
4.3. Effects of LP Stress on Organic Acids in Roots
4.4. Effects of LP Stress on Phytohormones in Roots
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Indices | GN | LD | QS | |||
---|---|---|---|---|---|---|
LP | NP | LP | NP | LP | NP | |
Plant height/cm | 57.40 ± 10.11 b | 63.87 ± 8.25 a | 56.23 ± 10.11 b | 59.28 ± 10.27 a | 53.10 ± 4.99 b | 55.48 ± 5.48 a |
Leaf length/cm | 1.73 ± 0.10 b | 1.94 ± 0.16 a | 1.34 ± 0.13 b | 1.57 ± 0.12 a | 1.23 ± 0.23 b | 1.45 ± 0.11 a |
Leaf width/cm | 0.85 ± 0.11 b | 0.93 ± 0.04 a | 0.69 ± 0.09 b | 0.74 ± 0.05 a | 0.61 ± 0.05 b | 0.74 ± 0.05 a |
Leaf area/cm2 | 0.49 ± 0.09 b | 0.60 ± 0.07 a | 0.31 ± 0.07 b | 0.39 ± 0.03 a | 0.25 ± 0.07 b | 0.36 ± 0.02 a |
Total length/cm | 3408.56 ± 6.09 a | 1712.10 ± 2.61 b | 3491.61 ± 4.23 a | 1569.48 ± 6.70 b | 2843.40 ± 3.01 a | 1438.60 ± 6.84 b |
Total surface area/cm2 | 46.03 ± 4.24 b | 57.74 ± 0.98 a | 44.88 ± 5.80 b | 66.98 ± 0.94 a | 33.07 ± 5.72 b | 53.10 ± 1.14 a |
Total volume/cm3 | 1.36 ± 0.53 b | 4.09 ± 0.32 a | 1.30 ± 0.34 b | 4.09 ± 0.65 a | 0.81 ± 0.23 b | 3.45 ± 0.56 a |
Ground biomass/g·10 plant−1 | 8.56 ± 1.24 b | 9.57 ± 0.75 a | 5.47 ± 1.40 b | 10.39 ± 0.69 a | 6.48 ± 1.12 b | 10.09 ± 0.23 a |
Root biomass/g·10 plant−1 | 13.98 ± 1.91 a | 6.91 ± 0.95 b | 8.87 ± 0.98 a | 3.27 ± 0.22 b | 6.88 ± 0.23 a | 3.24 ± 0.18 b |
Root/shoot ratio | 1.67 ± 0.41 a | 0.73 ± 0.11 b | 1.72 ± 0.62 a | 0.31 ± 0.02 b | 1.08 ± 0.19 a | 0.33 ± 0.08 b |
Category | Metabolites | Fold Change (FC) | ||
---|---|---|---|---|
GN_LP vs. GN_NP | LD_LP vs. LD_NP | QS_LP vs. QS_NP | ||
Amino acids | L-histidine | - | 0.331 | - |
4-oxoproline | - | 0.675 | - | |
D-proline | - | 0.510 | - | |
L-saccharopine | - | - | 0.367 | |
L-tryptophan | - | - | 7.918 | |
Organic acids | Methylmalonic acid | 0.367 | - | - |
Methylmalonate | 0.221 | - | - | |
Malonic acid | - | 2.735 | - | |
Shikimic acid | - | - | 0.575 | |
Plant growth regulating substance | 5-Aminopentanoate | - | 0.621 | - |
Gibberellic acid | - | - | 0.361 | |
Brassinolide | - | - | 4.431 | |
Biotinoids | Biotin | - | 3.439 | - |
Desthiobiotin | - | 2.068 | - | |
Polyamine substances | Agmatine | - | 0.207 | - |
Nicotinic acids | Quinolinic acid | - | 0.538 | - |
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Xia, J.; Nan, L.; Wang, K.; Yao, Y. Comprehensive Dissection of Metabolites in Response to Low Phosphorus Stress in Different Root-Type Alfalfa at Seedling Stage. Agronomy 2024, 14, 1697. https://doi.org/10.3390/agronomy14081697
Xia J, Nan L, Wang K, Yao Y. Comprehensive Dissection of Metabolites in Response to Low Phosphorus Stress in Different Root-Type Alfalfa at Seedling Stage. Agronomy. 2024; 14(8):1697. https://doi.org/10.3390/agronomy14081697
Chicago/Turabian StyleXia, Jing, Lili Nan, Kun Wang, and Yuheng Yao. 2024. "Comprehensive Dissection of Metabolites in Response to Low Phosphorus Stress in Different Root-Type Alfalfa at Seedling Stage" Agronomy 14, no. 8: 1697. https://doi.org/10.3390/agronomy14081697