Toxic Effects of Bt-(Cry1Ab+Vip3Aa) Maize (“DBN3601T’’ Event) on the Asian Corn Borer Ostrinia furnacalis (Guenée) in Southwestern China
Abstract
:1. Introduction
2. Materials and Methods
2.1. Collection and Rearing of the ACB
2.2. Maize Variety for Testing
2.3. Determination of ACB Susceptibility to Insecticidal Proteins Expressed by the Bt Maizes
2.4. Determination of Insecticidal Protein Expression in Different Tissues of Bt Maize and Tissue Bioassays
2.5. Field Evaluation
2.6. Data Analysis
3. Results
3.1. Susceptibility of the ACB to Bt Insecticidal Proteins Expressed by the Bt Maize
3.2. Expression of Insecticidal Proteins in Different Tissues of Bt-(Cry1Ab+Vip3Aa) Maize
3.3. Field Evaluation for Resistance Level of the Bt Maize to ACB
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Inhibition. Effect | Bt Event (Protein) | N | LC50/GIC50 (95%FL) ng/g | LC95/GIC95 (95%FL) ng/g | Slope ± SE | χ2 | df |
---|---|---|---|---|---|---|---|
LC | DBN9936 (Cry1Ab) | 360 | 51.42 (45.11–58.90) a | 190.15 (150.32–261.01) a | 2.90 ± 0.25 | 15.83 | 13 |
DBN9501 (Vip3Aa) | 360 | >1591.20 | — | — | — | — | |
DBN3601T (Cry1Ab+Vip3Aa) | 360 | 46.85 (40.36–54.20) a | 217.97 (168.01–311.21) a | 2.46 ± 0.22 | 15.38 | 13 | |
GIC | DBN9936 (Cry1Ab) | 360 | 0.63 (0.01–1.66) A | 14.55 (9.90–19.26) A | 1.20 ± 0.23 | 3.10 | 13 |
DBN9501 (Vip3Aa) | 360 | >1591.20 | — | — | — | — | |
DBN3601T (Cry1Ab+Vip3Aa) | 360 | 0.30 (0.00–1.30) A | 14.99 (718–22.01) A | 0.97 ± 0.22 | 3.31 | 13 |
Tissue | Cry1Ab μg/g | Vip3Aa μg/g | Total Bt Protein μg/g |
---|---|---|---|
V6–V8 leaf | 82.35 ± 8.39 a | 31.86 ± 6.91 a | 114.20 ± 3.22 a |
VT tassel | 27.11 ± 3.32 b | 3.59 ± 0.11 b | 30.69 ± 3.41 b |
R1 silk | 3.17 ± 1.21 e | 0.60 ± 0.29 d | 3.77 ± 0.96 d |
R2 kernel | 5.45 ± 0.54 d | 3.48 ± 0.01 b | 8.92 ± 0.53 c |
R3 Stalk | 9.55 ± 1.57 c | 1.55 ± 0.70 c | 11.09 ± 2.09 c |
R3 cob | 9.15 ± 1.83 c | 1.84 ± 0.69 c | 10.99 ± 1.79 c |
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Li, H.; Wang, W.; Yang, X.; Kang, G.; Zhang, Z.; Wu, K. Toxic Effects of Bt-(Cry1Ab+Vip3Aa) Maize (“DBN3601T’’ Event) on the Asian Corn Borer Ostrinia furnacalis (Guenée) in Southwestern China. Agronomy 2024, 14, 1906. https://doi.org/10.3390/agronomy14091906
Li H, Wang W, Yang X, Kang G, Zhang Z, Wu K. Toxic Effects of Bt-(Cry1Ab+Vip3Aa) Maize (“DBN3601T’’ Event) on the Asian Corn Borer Ostrinia furnacalis (Guenée) in Southwestern China. Agronomy. 2024; 14(9):1906. https://doi.org/10.3390/agronomy14091906
Chicago/Turabian StyleLi, Haitao, Wenhui Wang, Xianming Yang, Guodong Kang, Zhenghao Zhang, and Kongming Wu. 2024. "Toxic Effects of Bt-(Cry1Ab+Vip3Aa) Maize (“DBN3601T’’ Event) on the Asian Corn Borer Ostrinia furnacalis (Guenée) in Southwestern China" Agronomy 14, no. 9: 1906. https://doi.org/10.3390/agronomy14091906