Contrasting Dynamic Photoprotective Mechanisms under Fluctuating Light Environments between an Andean and a Mesoamerican Genotype of Phaseolus vulgaris L.
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material
2.2. Dark and Light Adaptation of Leaves
2.3. Chlorophyll Fluorescence on Dark-Adapted and Light-Adapted Leaves
2.4. Non-Photochemical Quenching (NPQ) in Fluctuating Light
3. Data Analysis
4. Results
4.1. Variation in Chlorophyll Fluorescence of Dark- and Light-Adapted Leaves between an Andean and a Mesoamerican Common Bean Genotype
4.2. NPQ Induction Patterns in Andean (Calima) and Mesoamerican (Jamapa) Common Bean Genotypes
4.3. NPQ Relaxation in Andean (Calima) and Mesoamerican (Jamapa) Common Bean Genotypes
5. Discussion
6. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
Fo | Minimum fluorescence after dark adaptation |
Fm | Maximum fluorescence after dark adaptation |
Fv/Fm | Maximum quantum efficiency of PSII reaction centers after dark adaptation (1 − (Fo/Fm)) |
Fo′ | Minimum fluorescence after light adaptation |
Fm′ | Maximum fluorescence after light adaptation |
Fv′/Fm′ | Effective quantum efficiency of PSII reaction centers of after light adaptation (1 − (Fo′/Fm′)) |
Fs | Steady-state fluorescence (after light adaptation) |
ΦPS2 | Quantum efficiency of photosynthetic electron transport through PSII |
ΦCO2 | Quantum efficiencies of photosynthetic electron transport through CO2 assimilation (ΦCO2/ΦCO2) |
qP | Photochemical (qP) quenching coefficient |
qN | Non-photochemical (qN) quenching coefficient |
qL | Open reaction centers estimated from qP |
NPQ | Non-photochemical quenching |
A | CO2 assimilation |
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NPQ * | 1000 µmol m−2s−1 PAR | 1500 µmol m−2s−1 PAR | 2000 µmol m−2s−1 PAR | |||||||||
Genotype | Slope = ∆NPQ/∆t (min) | R2 | r | p-value | Slope = ∆NPQ/∆t (min) | R2 | r | p-value | Slope = ∆NPQ/∆t (min) | R2 | r | p-value |
Calima | 0.039 | 0.56 | 0.75 | 0.001 | 0.012 | 0.04 | 0.19 | >0.05 | 0.028 | 0.1 | 0.3 | >0.05 |
Jamapa | −0.0029 | 0.01 | −0.08 | >0.05 | 0.018 | 0.12 | 0.34 | >0.05 | 0.013 | 0.07 | 0.26 | >0.05 |
Assimilation | 1000 µmol m−2s−1 PAR | 1500 µmol m−2s−1 PAR | 2000 µmol m−2s−1 PAR | |||||||||
Genotype | Slope = ∆A/∆t (min) | R2 | r | p-value | Slope = ∆A/∆t (min) | R2 | r | p-value | Slope = ∆A/∆t (min) | R2 | r | p-value |
Calima | −0.08 | 0.02 | −0.14 | >0.05 | 0.23 | 0.78 | 0.88 | 0.05 | 0.28 | 0.2 | 0.44 | >0.05 |
Jamapa | 0.03 | 0.01 | −0.08 | >0.05 | 0.2 | 0.06 | 0.25 | >0.05 | 0.03 | 0.06 | 0.05 | >0.05 |
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Egesa, A.O.; Puengchanchaikul, V.; Vallejos, C.E.; Begcy, K. Contrasting Dynamic Photoprotective Mechanisms under Fluctuating Light Environments between an Andean and a Mesoamerican Genotype of Phaseolus vulgaris L. Agronomy 2024, 14, 1907. https://doi.org/10.3390/agronomy14091907
Egesa AO, Puengchanchaikul V, Vallejos CE, Begcy K. Contrasting Dynamic Photoprotective Mechanisms under Fluctuating Light Environments between an Andean and a Mesoamerican Genotype of Phaseolus vulgaris L. Agronomy. 2024; 14(9):1907. https://doi.org/10.3390/agronomy14091907
Chicago/Turabian StyleEgesa, Andrew Ogolla, Voraruthai Puengchanchaikul, C. Eduardo Vallejos, and Kevin Begcy. 2024. "Contrasting Dynamic Photoprotective Mechanisms under Fluctuating Light Environments between an Andean and a Mesoamerican Genotype of Phaseolus vulgaris L." Agronomy 14, no. 9: 1907. https://doi.org/10.3390/agronomy14091907