Uncovering Fusarium Species Associated with Fusarium Wilt in Chickpeas (Cicer arietinum L.) and the Identification of Significant Marker–Trait Associations for Resistance in the International Center for Agricultural Research in the Dry Areas’ Chickpea Collection Using SSR Markers
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Fungal Pathogens
2.2. Fusarium Infection
2.3. Polymerase Chain Reaction (PCR) Analysis
2.4. Genetic Diversity and Population Structure Analysis
2.5. Association Mapping for the Determination of Marker–Trait Associations (MTA)
3. Results
3.1. The Identification of Pathogenic Fungi Isolates
3.2. Genetic Diversity for FW Resistance
3.3. A SSR-Based Molecular Diversity Analysis
3.4. A Structure Analysis and Cluster Analysis
3.5. Identifying the Associations Related to FW Resistance
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Isolate | Predicted Fungi | Strain | Similarity, % |
---|---|---|---|
1 | Neocosmospora solani (previously the F. solani) | NRRL 46643 | 99.71 |
2 | Neocosmospora nelsonii (previously the F. caricae) | CBS 309.75 | 99.58 |
3 | Neocosmospora falciformis (previously the F. falciforme) | NRRL 32798 | 99.8 |
4 | Fusarium brachygibbosum | NRRL 34033 | 99.4 |
5 | Neocosmospora falciformis (previously the F. falciforme) | NRRL 32798 | 99.8 |
6 | Neocosmospora falciformis (previously the F. falciforme) | NRRL 32339 | 99.9 |
7 | Neocosmospora brevis (previously the F. brevis) | F93 | 99.69 |
8 | Fusarium gossypinum | CBS 116613 | 100 |
№ | Genotype | Type | FW Response | № | Genotype | Type | FW Response * |
---|---|---|---|---|---|---|---|
1 | 17102 | Germplasm line | MR | 49 | 17174 | Germplasm line | R |
2 | 17103 | Germplasm line | MR | 50 | 17201 | Variety | R |
3 | 17104 | Variety | R | 51 | 17206 | Germplasm line | R |
4 | 17106 | Germplasm line | R | 52 | 17212 | Germplasm line | S |
5 | 17108 | Germplasm line | S | 53 | 17222 | Variety | S |
6 | 17109 | Germplasm line | MS | 54 | 17223 | Germplasm line | MR |
7 | 17110 | Variety | MR | 55 | 17225 | Variety | R |
8 | 17111 | Germplasm line | R | 56 | 17236 | Germplasm line | R |
9 | 17112 | Germplasm line | R | 57 | 17244 | Germplasm line | MR |
10 | 17114 | Germplasm line | R | 58 | 17265 | Germplasm line | R |
11 | 17115 | Germplasm line | R | 59 | 17269 | Germplasm line | R |
12 | 17116 | Variety | R | 60 | 17270 | Variety | R |
13 | 17117 | Germplasm line | R | 61 | M1 | Germplasm line | R |
14 | 17118 | Germplasm line | R | 62 | M2 | Germplasm line | R |
15 | 17120 | Germplasm line | S | 63 | M3 | Germplasm line | MR |
16 | 17121 | Germplasm line | MR | 64 | M4 | Germplasm line | R |
17 | 17123 | Germplasm line | MS | 65 | M5 | Germplasm line | R |
18 | 17124 | Germplasm line | R | 66 | M6 | Germplasm line | R |
19 | 17125 | Variety | R | 67 | M7 | Germplasm line | R |
20 | 17126 | Germplasm line | R | 68 | M8 | Germplasm line | R |
21 | 17127 | Germplasm line | R | 69 | M9 | Germplasm line | R |
22 | 17130 | Germplasm line | R | 70 | M10 | Germplasm line | R |
23 | 17132 | Germplasm line | R | 71 | M11 | Variety | R |
24 | 17135 | Germplasm line | R | 72 | M12 | Germplasm line | R |
25 | 17136 | Germplasm line | MR | 73 | M13 | Germplasm line | R |
26 | 17139 | Germplasm line | R | 74 | M14 | Germplasm line | R |
27 | 17141 | Germplasm line | R | 75 | M15 | Germplasm line | R |
28 | 17142 | Germplasm line | R | 76 | M16 | Germplasm line | MR |
29 | 17143 | Variety | S | 77 | M17 | Germplasm line | R |
30 | 17144 | Germplasm line | MR | 78 | M18 | Variety | R |
31 | 17145 | Germplasm line | MS | 79 | M19 | Germplasm line | R |
32 | 17147 | Germplasm line | R | 80 | M20 | Germplasm line | R |
33 | 17148 | Germplasm line | MR | 81 | M21 | Germplasm line | R |
34 | 17150 | Germplasm line | MR | 82 | M22 | Germplasm line | R |
35 | 17151 | Germplasm line | MS | 83 | M23 | Germplasm line | R |
36 | 17153 | Germplasm line | MS | 84 | M24 | Germplasm line | MR |
37 | 17154 | Germplasm line | R | 85 | M25 | Variety | MR |
38 | 17156 | Germplasm line | MS | 86 | M26 | Germplasm line | R |
39 | 17157 | Germplasm line | MS | 87 | M27 | Germplasm line | R |
40 | 17158 | Variety | MR | 88 | M28 | Germplasm line | R |
41 | 17159 | Germplasm line | R | 89 | M29 | Germplasm line | R |
42 | 17161 | Variety | R | 90 | M30 | Germplasm line | MS |
43 | 17162 | Germplasm line | R | 91 | M31 | Germplasm line | R |
44 | 17163 | Germplasm line | R | 92 | M32 | Germplasm line | R |
45 | 17165 | Germplasm line | R | 93 | M33 | Germplasm line | R |
46 | 17166 | Germplasm line | R | 94 | M34 | Germplasm line | R |
47 | 17169 | Germplasm line | R | 95 | M35 | Variety | R |
48 | 17172 | Germplasm line | R | 96 | M36 | Germplasm line | R |
MTA | Locus | p-Value | R2 | LG | Reference * |
---|---|---|---|---|---|
GLM mean in year 2023 | |||||
TA42 | A | 0.0028 | 0.0911 | LG5 | [36] |
TA125 | A | 0.0043 | 0.0834 | LG1 | [36,37] |
TR2 | B | 0.0096 | 0.0692 | LG6 | [35] |
TA125 | B | 0.0098 | 0.0688 | LG1 | [36,37] |
TA37 | D | 0.01 | 0.0684 | LG2 | [35] |
TAASH | B | 0.0108 | 0.067 | LG1 | [37] |
MLM mean in year 2023 | |||||
TA42 | A | 0.0028 | 0.0912 | LG5 | [36] |
TA125 | A | 0.0043 | 0.0834 | LG1 | [36,37] |
TA125 | B | 0.0098 | 0.0688 | LG1 | [36,37] |
TA37 | D | 0.0111 | 0.0666 | LG2 | [35] |
TAASH | B | 0.0165 | 0.0596 | LG1 | [37] |
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Murodova, S.M.; Bozorov, T.A.; Aytenov, I.S.; Ochilov, B.O.; Qulmamatova, D.E.; Salakhutdinov, I.B.; Isokulov, M.Z.; Khalillaeva, G.O.; Azimova, L.A.; Meliev, S.K. Uncovering Fusarium Species Associated with Fusarium Wilt in Chickpeas (Cicer arietinum L.) and the Identification of Significant Marker–Trait Associations for Resistance in the International Center for Agricultural Research in the Dry Areas’ Chickpea Collection Using SSR Markers. Agronomy 2024, 14, 1943. https://doi.org/10.3390/agronomy14091943
Murodova SM, Bozorov TA, Aytenov IS, Ochilov BO, Qulmamatova DE, Salakhutdinov IB, Isokulov MZ, Khalillaeva GO, Azimova LA, Meliev SK. Uncovering Fusarium Species Associated with Fusarium Wilt in Chickpeas (Cicer arietinum L.) and the Identification of Significant Marker–Trait Associations for Resistance in the International Center for Agricultural Research in the Dry Areas’ Chickpea Collection Using SSR Markers. Agronomy. 2024; 14(9):1943. https://doi.org/10.3390/agronomy14091943
Chicago/Turabian StyleMurodova, Sojida M., Tohir A. Bozorov, Ilkham S. Aytenov, Bekhruz O. Ochilov, Dilafruz E. Qulmamatova, Ilkhom B. Salakhutdinov, Marufbek Z. Isokulov, Gavkhar O. Khalillaeva, Laylo A. Azimova, and Sodir K. Meliev. 2024. "Uncovering Fusarium Species Associated with Fusarium Wilt in Chickpeas (Cicer arietinum L.) and the Identification of Significant Marker–Trait Associations for Resistance in the International Center for Agricultural Research in the Dry Areas’ Chickpea Collection Using SSR Markers" Agronomy 14, no. 9: 1943. https://doi.org/10.3390/agronomy14091943