Influence of Exogenous Ethylene and Mechanical Damage on Gene Expression and Physiological Parameters of Maize Hybrids
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material, Treatments, and Sampling
2.2. Method of BXD Isolation, Sample Preparation, and Measurement
2.3. SOD Measurement Method
2.4. CAT Measurement Method
2.5. MDA Measurement Method
2.6. Total RNA Isolation and cDNA Synthesis
2.7. qPCR Analysis
2.8. Statistical Analysis
3. Results
3.1. Results of Benzoxazinoid (BXD) Measurements
3.2. Results of SOD Measurement
3.3. Results of CAT Measurement
3.4. Results of MDA Measurements
3.5. Results of Gene Expression Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Chemical Compound | Treatment | Sampling 2 h after Treatments | Sampling 4 h after Treatments |
---|---|---|---|
DIMBOA | Control | 27.93 ± 8.56 a1 | 12.17 ± 2.79 a1 |
Mechanical damage | 34.62 ± 8.43 a1 | 32.62 ± 6.54 b1 | |
Ethylene | 38.87 ± 9.08 a1 | 28.56 ± 5.27 b1 | |
HMBOA | Control | 12.36 ± 2.10 a1 | 11.90 ± 2.57 a1 |
Mechanical damage | 19.41 ± 2.01 a1 | 13.88 ± 2.55 a1 | |
Ethylene | 15.00 ± 3.75 a1 | 15.43 ± 1.77 a1 | |
DIMBOA+ HMBOA | Control | 40.29 ± 7.75 a1 | 24.07 ± 5.27 a1 |
Mechanical damage | 54.04 ± 8.43 a1 | 46.50 ± 6.52 b1 | |
Ethylene | 53.87 ± 8.79 a1 | 43.99 ± 4.99 b1 |
Chemical Compound | Treatment | Sampling 2 h after Treatments | Sampling 4 h after Treatments |
---|---|---|---|
DIMBOA | Control | 26.45 ± 6.76 a1 | 72.11 ± 7.87 b2 |
Mechanical damage | 15.07 ± 2.91 a1 | 44.51 ± 8.36 ab2 | |
Ethylene | 26.87 ± 4.31 a1 | 36.43 ± 8.01 a1 | |
HMBOA | Control | 27.80 ± 9.19 a1 | 18.28 ± 3.03 a1 |
Mechanical damage | 55.47 ± 24.85 a1 | 36.84 ± 7.57 ab1 | |
Ethylene | 23.72 ± 2.36 a1 | 23.32 ± 3.80 a1 | |
DIMBOA+ HMBOA | Control | 54.25 ± 7.69 a1 | 90.39 ± 6.47 b2 |
Mechanical damage | 70.53 ± 23.51 a1 | 81.35 ± 3.47 ab2 | |
Ethylene | 50.59 ± 3.72 a1 | 59.75 ± 4.41 a1 |
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Makleit, P.; Gulyás, G.; Czeglédi, L.; Veres, S. Influence of Exogenous Ethylene and Mechanical Damage on Gene Expression and Physiological Parameters of Maize Hybrids. Agronomy 2024, 14, 1950. https://doi.org/10.3390/agronomy14091950
Makleit P, Gulyás G, Czeglédi L, Veres S. Influence of Exogenous Ethylene and Mechanical Damage on Gene Expression and Physiological Parameters of Maize Hybrids. Agronomy. 2024; 14(9):1950. https://doi.org/10.3390/agronomy14091950
Chicago/Turabian StyleMakleit, Péter, Gabriella Gulyás, Levente Czeglédi, and Szilvia Veres. 2024. "Influence of Exogenous Ethylene and Mechanical Damage on Gene Expression and Physiological Parameters of Maize Hybrids" Agronomy 14, no. 9: 1950. https://doi.org/10.3390/agronomy14091950