Genome-Wide Identification of the Shaker Potassium Channel Family in Chinese Cabbage and Functional Studies of BrKAT1 in Yeast
Abstract
:1. Introduction
2. Materials and Methods
2.1. Genome-Wide Identification of Shaker K+ Channel Family Members
2.2. Phylogenetic Tree Analysis, Gene Structure, and Chromosome Position
2.3. Intraspecific Collinearity
2.4. Interspecific Collinearity
2.5. Promoter Analysis
2.6. Plant Culture, Gene Cloning, Vector Construction, and Yeast Transformation
2.7. Yeast Assays
3. Results
3.1. Phylogenetic Tree Analysis
3.2. Protein Domains of the Chinese Cabbage Shaker K+ Channel
3.3. Chromosome Position and Intraspecific Collinearity of Shaker K+ Channels in Chinese Cabbage
3.4. Interspecific Collinearity
3.5. Promoter Analysis
3.6. Both BrKAT1 Proteins Have Potassium Absorption Functions, and the N-Terminus Is Necessary for Maintaining Potassium Channel Function
3.7. BrKAT1 Enhances Cell Salt Tolerance
4. Discussion
4.1. Gene Expansion Exists in the Shaker K+ Channel of Chinese Cabbage, and the Segmental Duplication of the Genes in Group II and Group V as the Main Reason for the Above Phenomenon
4.2. The Two Homologs of Group II, BrKAT1.1 and BrKAT1.2, Have Similar Potassium Uptake and Salt Tolerance Ability but Possess Distinct Promoter Elements
4.3. The N-Terminus Is Necessary for Maintaining the Function of BrKAT1
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Name | Primers (5′ to 3′) |
---|---|
BrKAT1.1-F | actatagggaatattaagcttATGTCCATCTCTTGCACCAGAAACT |
BrKAT1.1-R | tgatggatatctgcagaattcTCAGCTTGATGAGAAAAACAAATGA |
BrKAT1.2Ncut-F | actatagggaatattaagcttATGTGGCTTGTCCTTCTAGTTATTTACT |
BrKAT1.2Ncut-R | tgatggatatctgcagaattcTCAATGTCTACCTTCAAACTCAATTTG |
BrKAT1.2-F | actatagggaatattaagcttATGCCAATCTCTTGTACCAGAAACT |
BrKAT1.2-R | tgatggatatctgcagaattcTCAATGTCTACCTTCAAACTCAATTTG |
Protein Name | Gene_ID | Length | MW (kDa) | pI |
---|---|---|---|---|
BrKAT3.2 | BraA01g005450.3.5C | 651 | 74.51 | 7.24 |
BrAKT5 | BraA01g005630.3.5C | 876 | 98.16 | 6.94 |
BrKAT2.1 | BraA01g009830.3.5C | 787 | 90.79 | 9.18 |
BrAKT2/3 | BraA01g012890.3.5C | 862 | 97.92 | 6.73 |
BrSKOR1.2 | BraA03g031580.3.5C | 723 | 82.54 | 6.84 |
BrKAT3.1 | BraA03g058380.3.5C | 656 | 75.19 | 8.19 |
BrAKT1 | BraA04g019690.3.5C | 860 | 97.44 | 7.9 |
BrSKOR1.1 | BraA05g042590.3.5C | 829 | 94.05 | 6.65 |
BrKAT1.2 | BraA06g043430.3.5C | 688 | 79.05 | 6.4 |
BrGORK | BraA07g005880.3.5C | 817 | 94.11 | 6.05 |
BrKAT2.2 | BraA08g013430.3.5C | 670 | 76.94 | 8.67 |
BrKAT1.1 | BraA09g022550.3.5C | 657 | 75.69 | 7.11 |
BrAKT6 | BraA09g054710.3.5C | 877 | 98 | 6.64 |
Gene Pairs | Ks | Ka | Ka/Ks |
---|---|---|---|
BrKAT1.1-BrKAT2.2 | 0.17 | 1.11 | 0.15 |
BrKAT2.2-BrKAT1.2 | 0.21 | 1.16 | 0.18 |
BrKAT1.1-BrKAT1.2 | 0.05 | 0.30 | 0.18 |
BrKAT2.1-BrKAT2.2 | 0.09 | 0.47 | 0.20 |
BrKAT1.2-BrKAT2.1 | 0.16 | 1.48 | 0.11 |
BrSKOR1.1-BrSKOR1.2 | 0.04 | 0.35 | 0.10 |
BrAKT6-BrKAT3.1 | 0.74 | 4.65 | 0.16 |
BrKAT3.1-BrKAT3.2 | 0.06 | 0.22 | 0.28 |
BrAKT6-BrKAT3.2 | 0.56 | 4.55 | 0.12 |
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Zhou, J.-Y.; Gu, Z.-C.; Hao, D.-L. Genome-Wide Identification of the Shaker Potassium Channel Family in Chinese Cabbage and Functional Studies of BrKAT1 in Yeast. Agronomy 2024, 14, 1954. https://doi.org/10.3390/agronomy14091954
Zhou J-Y, Gu Z-C, Hao D-L. Genome-Wide Identification of the Shaker Potassium Channel Family in Chinese Cabbage and Functional Studies of BrKAT1 in Yeast. Agronomy. 2024; 14(9):1954. https://doi.org/10.3390/agronomy14091954
Chicago/Turabian StyleZhou, Jin-Yan, Ze-Chen Gu, and Dong-Li Hao. 2024. "Genome-Wide Identification of the Shaker Potassium Channel Family in Chinese Cabbage and Functional Studies of BrKAT1 in Yeast" Agronomy 14, no. 9: 1954. https://doi.org/10.3390/agronomy14091954