Construction of Microbial Consortium to Enhance Cellulose Degradation in Corn Straw during Composting
Abstract
:1. Introduction
2. Materials and Methods
2.1. Isolation and Purification Microbial Strains
2.2. Screening and Identification of Microbial Strains
2.2.1. Primary Screening
2.2.2. Rescreening
2.3. Sequencing and Identification
2.4. Compatibility Test
2.5. Construction of Microbial Strain Consortium
2.5.1. Enzyme Activity and Cellulose Degradation Rate of Two-Microbial-Strain Combinations
2.5.2. Enzyme Activity and Cellulose Degradation Rate of Three-Microbial-Strain Combinations
2.6. pH Changes of Combined Microbial Strains
2.7. Composting Effect of Selected Microbial Strain Combinations for In Situ Fermentation
2.8. Statistical Analysis
3. Results
3.1. Screening and Identification of Corn-Straw-Degrading Microbial Strains
3.2. Enzyme Activity and Degradation Rate of Corn Straw Using Single Strain
3.3. Compatibility Test and Enzyme Activity of Combined Microbial Strains
3.4. Degradation Rate of Corn Straw Using Combined Microbial Strains
3.5. pH Value Changes during the Degradation of Corn Straw by Combined Microbial Strains
3.6. Changes in Physicochemical Properties during In Situ Corn Straw Composting
3.6.1. Temperature Changes with Different Microbial Inoculants
3.6.2. Moisture Content Changes with Different Microbial Inoculants
3.6.3. pH Changes during with Different Microbial Inoculants
3.6.4. E4/E6 Changes Value with Different Microbial Inoculants
3.6.5. Seed Germination Index Changes with Different Microbial Inoculants
4. Discussion
4.1. Identification and Screening of Microbial Strains
4.2. Corn Straw Composting In Situ
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Inoculant | Microbial Strains | ||
---|---|---|---|
Fungi | Bacteria | Actinomycetes | |
1 (T1) | A1 + A4 + A5 | B5 + B6 | C |
2 (T2) | A1 + A4 + A5 | B5 + B7 | C |
3 (T3) | A1 + A4 + A5 | B6 + B7 | C |
4 (T4) | A1 + A4 | B5 + B6 + B7 | C |
5 (T5) | A1 + A5 | B5 + B6 + B7 | C |
6 (T6) | A4 + A5 | B5 + B6 + B7 | C |
7 (T7) | A1 + A4 + A5 | B5 + B6 + B7 | C |
CK | Distilled water |
Strain Label | D/d (cm) |
---|---|
A1 | 5.95 ± 0.63 bc |
A2 | 5.89 ± 0.70 bc |
A3 | 7.37 ± 1.16 abc |
A4 | 5.06 ± 1.93 c |
A5 | 6.78 ± 1.07 abc |
B1 | 3.11 ± 0.12 b |
B2 | 3.20 ± 0.12 b |
C | 2.60 ± 0.06 b |
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Li, J.; Li, J.; Yang, R.; Yang, P.; Fu, H.; Yang, Y.; Liu, C. Construction of Microbial Consortium to Enhance Cellulose Degradation in Corn Straw during Composting. Agronomy 2024, 14, 2107. https://doi.org/10.3390/agronomy14092107
Li J, Li J, Yang R, Yang P, Fu H, Yang Y, Liu C. Construction of Microbial Consortium to Enhance Cellulose Degradation in Corn Straw during Composting. Agronomy. 2024; 14(9):2107. https://doi.org/10.3390/agronomy14092107
Chicago/Turabian StyleLi, Jie, Juan Li, Ruopeng Yang, Ping Yang, Hongbo Fu, Yongchao Yang, and Chaowei Liu. 2024. "Construction of Microbial Consortium to Enhance Cellulose Degradation in Corn Straw during Composting" Agronomy 14, no. 9: 2107. https://doi.org/10.3390/agronomy14092107