Extracellular Vesicle Transplantation Is Beneficial for Acute Kidney Injury
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Cell Phenotype Characterization
2.3. Endothelial Progenitor Cell Colony Formation Assay
2.4. Extracellular Vesicle Isolation and Characterizations
2.5. EV Labeling and Tracking
2.6. Flow Cytometry Analysis of EVs
2.7. Animals
2.8. Renal Ischemia-Reperfusion Injury Induction
2.9. Immunohistochemistry Analysis
2.10. Library Preparation, Sequencing, and Bioinformatics Analysis
2.11. Statistical Analysis
3. Results
3.1. Characterization of Regeneration-Associated Cells
3.2. Characterization of EVs
3.3. RACev Transplantation Improved Kidney Function
3.4. RACev Therapy Preserved from Kidney Fibrosis
3.5. Regulatory Role of RACev on Epithelial Mesenchymal Transition, Inflammation, and Hypoxia after Ischemic Injury
3.6. RACev Restored Capillary Densities
3.7. Preferential RACev Accumulation in the Injured Kidney
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Salybekov, A.A.; Okamura, S.; Ohtake, T.; Hidaka, S.; Asahara, T.; Kobayashi, S. Extracellular Vesicle Transplantation Is Beneficial for Acute Kidney Injury. Cells 2024, 13, 1335. https://doi.org/10.3390/cells13161335
Salybekov AA, Okamura S, Ohtake T, Hidaka S, Asahara T, Kobayashi S. Extracellular Vesicle Transplantation Is Beneficial for Acute Kidney Injury. Cells. 2024; 13(16):1335. https://doi.org/10.3390/cells13161335
Chicago/Turabian StyleSalybekov, Amankeldi A., Shigeaki Okamura, Takayasu Ohtake, Sumi Hidaka, Takayuki Asahara, and Shuzo Kobayashi. 2024. "Extracellular Vesicle Transplantation Is Beneficial for Acute Kidney Injury" Cells 13, no. 16: 1335. https://doi.org/10.3390/cells13161335