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Review

Aquaporins in Cereals—Important Players in Maintaining Cell Homeostasis under Abiotic Stress

by
Marzena Małgorzata Kurowska
Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiellońska 28, 40-032 Katowice, Poland
Genes 2021, 12(4), 477; https://doi.org/10.3390/genes12040477
Submission received: 19 February 2021 / Revised: 18 March 2021 / Accepted: 23 March 2021 / Published: 25 March 2021
(This article belongs to the Special Issue Genetics and Physiology of Multiple-Stress Tolerance in Crops)
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Abstract

:
Cereal productivity is reduced by environmental stresses such as drought, heat, elevated CO2, salinity, metal toxicity and cold. Sometimes, plants are exposed to multiple stresses simultaneously. Plants must be able to make a rapid and adequate response to these environmental stimuli in order to restore their growing ability. The latest research has shown that aquaporins are important players in maintaining cell homeostasis under abiotic stress. Aquaporins are membrane intrinsic proteins (MIP) that form pores in the cellular membranes, which facilitate the movement of water and many other molecules such as ammonia, urea, CO2, micronutrients (silicon and boron), glycerol and reactive oxygen species (hydrogen peroxide) across the cell and intercellular compartments. The present review primarily focuses on the diversity of aquaporins in cereal species, their cellular and subcellular localisation, their expression and their functioning under abiotic stresses. Lastly, this review discusses the potential use of mutants and plants that overexpress the aquaporin-encoding genes to improve their tolerance to abiotic stress.

1. Introduction

Aquaporins (AQP), which are members of the major intrinsic proteins (MIP) superfamily, facilitate the bi-directional flux of water and non-aqua substrates across the cell membranes [1,2,3,4]. The history of studies on AQPs is quite interesting, and two groups were involved in the breakthrough research: Agre’s and Benga’s group. The first water channel protein that was discovered and characterised from human erythrocytes was CHannel-forming Integral membrane Protein of 28 kDa (CHIP28), which was later renamed Aquaporin-1 (AQP1) after its water conductivity was shown by expression in Xenopus oocytes [5]. The Nobel Prize in Chemistry for 2003 was awarded to two scientists, Peter Agre and Roderick MacKinnon, who had made fundamental discoveries concerning the channels in the cell membranes. Additionally, the water channel protein (WCP) had also been identified in situ in the human red blood cell (RBC) membrane earlier by Benga’s group [6,7]. In turn, the first AQP in plants was identified in soybeans—GmNOD26 [8], while the ability to conduct water in plants was demonstrated in Arabidopsis for AtTIP1;1 (gamma-TIP) by its heterologous expression in the Xenopus oocytes for the first time [9]. These transmembrane proteins form pores in the lipid bilayers of archaea, bacteria, fungi, plants, non-mammalian metazoans and mammalians including humans. Although it has been suggested that AQPs are multifunctional proteins, their structure is unique across all of the kingdoms of living organisms. The molecular weight of the members of the AQP family ranges from 23 to 31 kDa [10]. Despite some sequence diversity, all MIPs share a similar three-dimensional structure that consists of six transmembrane helices (S1 to S6) with five connecting inter-helical loops (A to E) and two half helices that contain one highly conserved Asn-Pro-Ala (NPA) motif each, which forms a functional pore (Figure 1). The hydrophobic NPA motif is localised at the first intracellular (loop B) and the third extracellular loops (loop E) [10]. The NPA motif and the aromatic/arginine selectivity filter (Ar/R SF) that includes four amino acids (aa) towards the extracellular side form two narrow regions within a channel. These two constricted regions are very important to the transport selectivity of the channels, which were predicted based on structural knowledge combined with simulation studies [11,12]. The NPA motif not only plays a role in regulating membrane transport, but also in protein localisation [13].
All of the MIP sequences take on a typical hour-glass MIP helical fold with six transmembrane helices and two half-helices [15]. This fold was maintained during evolution with the conservation of around 40 positions within the transmembrane region [15]. The stability of the functionally important half-helix is modulated by stabilising the intra-helical salt-bridge interaction and/or two helix-destabilising residues glycine and proline, which was demonstrated by analysing the loop E region of 1468 MIP sequences and their structural models from six different groups of organisms [16]. In the cell membrane, the MIPs are grouped as homotetramers that are located in the lipid bilayer (Figure 2). Each monomer functions independently as a single channel pore [17]. In plants, the crystal structure of only two MIPs is available in the Protein Data Bank [18]; the spinach (Spinacia oleracea) aquaporin SoPIP2;1 was in its closed confirmation at a 2.1 Å resolution and its open confirmation at a 3.9 Å resolution [19], and the Arabidopsis aquaporins AtPIP2;4 at a 3.7 Å resolution [20]. All of these were determined using X-ray diffraction and exhibited a structural identity. When the crystal structure of a protein is not available, it is possible to use the bioinformatic tools such as Phyre2 to predict and analyse the protein structures, and then use MOLES 2.5 to quickly locate and characterise the pores [21].
The functions and regulation of the majority of MIP channels have not yet been fully characterised [15]. Some AQP not only facilitate the transport of water but also non-aqua substrates such as ammonia, antimony, arsenite, carbon dioxide (CO2), formamide, glycerol, hydrogen peroxide (H2O2), lactic acid, micronutrients (silicon and boron), oxygen (O2) and urea [10,22]. In plants, AQPs are present in almost all organs including the roots, leaves, stems, flowers, fruits, seeds, dry seeds [2,23,24], pollen [25,26,27,28], anther and specific cells such as guard cells [29,30].
In field conditions, crops are challenged by a wide range of abiotic stresses. Environmental stresses such as drought, adverse temperatures (cold or heat) and salinity are major causes of yield losses in crops worldwide [31,32,33,34,35]. The impact on yields results in a decrease in the harvest index, a shortened life cycle of crops and changes in the seed number, size and composition. Furthermore, these effects are more severe when the stress occurs during the reproductive stage of a plant [35]. In nature, different stresses often occur together, which further exacerbates their effect. Episodes of prolonged drought coupled with heat waves is one of the possible stress combinations that affects crop yield [35]. The response of plants is quite complicated, and many mechanisms are initiated simultaneously in order to restore cellular homeostasis and promote survival [36]. All of the main abiotic stresses such as drought, cold, heat and salinity cause an imbalance in the water status in cells, tissues and whole plants. Regulating the flow of water through the membranes is one of the main mechanisms via which cells can maintain their homeostasis under stress conditions and water channels that are called aquaporins (AQP) are involved in this process [37].
The role of aquaporin in physiological processes, stress tolerance and their regulation in plants has been extensively and recently reviewed [38,39,40,41,42,43,44,45,46]. The aim of this manuscript is to focus on the aquaporins only in cereal species, their diversity, expression pattern and roles under abiotic stress. Cereals are important plants in agriculture, which might be used as human food, animal feed and in the brewing industry. The potential use of mutants and plants that overexpress the aquaporin-encoding genes to improve cereals tolerance to abiotic stress is also discussed.

2. Diversity of Aquaporins in Cereal Species

A large number of MIP genes have been identified in the genomes of different plant species. In plants, the MIP family contains around 2008 members that are deposited in the MIPDB (Major Intrinsic Proteins Database) [47], and their sequences range from 35 to 854 aa. Depending on the membrane localisation and amino acid sequence, the MIPs in higher plants including AQPs are typically divided into five subfamilies: the plasma membrane intrinsic proteins (PIP), tonoplast intrinsic proteins (TIP), nodulin-26-like proteins (NIP), small, basic intrinsic proteins (SIP) and the uncategorised X intrinsic proteins (XIP) [10]. However, in the monocot plant species, the XIP subfamily was lost during the evolution [41]. The PIPs, NIPs and XIPs are mainly localised in the plasma membrane, while the TIPs are localised in the tonoplast—a membrane of the vacuole. The SIPs and some NIPs have been found localising to the endoplasmic reticulum [22,28,48]. Some TIPs and SIPs have been predicted to be localised to the inner envelope and thylakoids [22]. Such a location was detected for TIP1;1, TIP1;2 and TIP2;1 in Arabidopsis [49,50,51]. In cereals, the number of MIP sequences that have been identified in genomes ranges from 33 to 113 in rice and wheat, respectively (Table 1). In other plant species, for example in Arabidopsis (Arabidopsis thaliana), cotton (Gossypium arboretum), cucumber (Cucumis sativus L.), olive trees (Olea europaea var. sylvestris and cv. Picual), potato (Solanum tuberosum L.), tabacco (Nicotiana tabacum) and tomato (Solanum lycopersicum L.), a large number of AQP genes have also been identified in genome 38, 53, 39, 52, 79, 41 and 47, respectively [52,53,54,55,56,57,58]. Throughout evolution, plants have kept a high number of AQPs and regulation mechanisms, and it might be an advantage when facing developmental and environmental challenges [59].

3. Study of the Expression and Role of Aquaporins in Maintaining the Cell Homeostasis of Cereals under Abiotic Stress

Plants are immobile; consequently, a quick and adequate change of the physiological processes in response to changes in environmental conditions is essential for their growth and productivity [68]. In plants, AQPs mediate water transport in both the roots and shoots, which is associated with the impact on plant hydraulics, transpiration and soil water conservation [67]. The function of aquaporins during the growth of plants and their abiotic stress responses can generally be considered as being dependent on (a) the developmental stage; (b) the organ: roots, leaves, stems, flowers, fruits, seeds, dry seeds, pollen, anther and specific cells such as the guard cells; (c) their subcellular localisation: plasma, tonoplast, endomembrane, peribacteroid membrane or (d) the transporting substrate: water, glycerol, CO2, urea, ammonia, H2O2, boron, silicon, arsenite, antimonite, lactic acid or O2 [10]. The variety of substrates that are transported by AQPs reflects the variety of physiological processes that are regulated by these proteins. Photosynthesis in plants is regulated by AQPs through their effect on CO2 transport in the membranes of the chloroplast and thylakoid [69,70,71]. Another example is that by facilitating the urea and NH4+/NH3 uptake, the AQPs ensure the N homeostasis [71,72]. The enabling of H2O2 diffusion by AQPs seems to be very important during the stress response when the reactive oxygen species are produced, while on the other hand the transport of this signalling molecule could have important functions in plant development and the regulation of a wide range of physiological processes [73]. Moreover, the AQPs are able to transport metalloids such as boron and silicon, which are important for the growth of plants. By contrast, the transport of toxic substances such as arsenic and antimony leads to cell homeostasis [74]. It has been postulated that specific AQPs are also involved in the movement of the stomata. These pores perform the gas exchange process, which enables the CO2 uptake for photosynthesis at the expense of the water loss by transpiration [30]. In turn, it is also known that some AQPs transport glycerol. This compound acts as an osmolyte and its transport via the aquaporins may be part of the stress response [75]. The accumulation of osmolytes increases the suction power in cells, which provides protection against water leakage [76]. Furthermore, during the seed maturation and germination processes, the tissue water content changes and these fluctuations are ensured by the AQPs [75].
In general, the changes in the mRNA expression levels lead to changes in protein abundance, but sometimes this is not the case [59]. The downregulation of the gene expression could lead to decrease in protein abundance, which could further lead to decrease in the membrane permeability. In turn, the change in the membrane permeability could be obtain also by regulating the AQPs gating. Changes in the gene expression in response to environmental stimuli can be an important component of system homeostasis on the one hand, while the gene regulatory differences in response to the environment can be an important component of the adaptation to stress condition on the other [77]. The expression of AQPs can be altered by abiotic factors, including drought, cold, heat and salinity in plants. Lastly, regulating the AQPs expression and changes in membrane permeability could also have an effect on crop productivity. Examples of studies of the expression of the AQP genes under different abiotic stress in cereals are presented in Table 2. Among the AQPs, the members of the PIP and TIP subfamilies have been analysed most often in order to determine their expression patterns under abiotic stress in cereals. This is quite important when comparing the profile of the expression of AQP genes to pay attention to the developmental stage and stress treatment conditions that are used in different experiments. Each AQP isoform gene might have an individual pattern of expression due to playing different functions during plant growth; e.g., it was shown that OsPIP1;2 functions in CO2 diffusion rather than the modulation of water transport in rice [78]. The quantitative reverse transcription PCR (qRT-PCR) until now has been the most widely used approach for the discovery of the pattern of AQP gene expression in cereal species under abiotic stress (Table 2).

3.1. Drought and Heat Stress

Drought stress is one of the major abiotic stress and causes huge losses in crop yield. The periods of drought that are caused by climate change and their frequency and severity will continue to be a growing problem around the world [76,85]. The main function of aquaporins under drought stress is to precisely regulate the movement of water among cells and tissues [86]. An analysis of the AQP gene expression in the leaf and/or root tissues in barley (Hordeum vulgare L.), foxtail millet (Setaria italica L.), rice (Oryza sativa L.) and wheat (Triticul aestivum L.) revealed significant changes in their response to drought or dehydration stress, which had been induced by the application of polyethylene glycol-6000 (PEG) (Table 2) [61,66,75,80,81]. Applying 20% PEG is equivalent to osmotic potential levels of −1.09 MPa [87]. Under drought stress, a downregulation of the AQP genes in leaf tissue can lead to a decrease in the water permeability of the membrane to avoid water loss. This expression pattern has been shown in the leaves for some TIP family member genes in barley (Hordeum vulgare L.) after ten days of severe drought treatment (Table 2) [80]. Among them, HvTIP1;1, HvTIP1;2 and HvTIP2;3 have been proven experimentally by using their in vitro expression in yeast that are able to transport water [88]. Among cereal species, the same profile of expression has been observed for OsTIP1;1 and OsTIP2;2 in rice leaves after 10 h of treatment with 15% PEG-6000 [75].
The correlation between the root water uptake capacity (i.e., the root hydraulic conductivity Lpr) and AQP gene expression has been observed in many studies [89,90,91,92,93]. The first clue that aquaporins might play a major role in the overall root water uptake came from an experiment in which it was shown that Hg2+ decreased a plant’s root hydraulic conductivity, and Hg2+ is one of the inhibitors of aquaporins [94]. Aquaporin activity could affect root hydraulic properties under drought stress [82]. The contribution of aquaporins to the Lpr was generally high (up to 79% under well-watered conditions and 85% under drought stress) and was also differentially regulated under drought in rice [82]. It was suggested that the upregulation of root aquaporin expression could prevent inhibition of transpiration by increasing the root water flow [95,96]. The expression of OsPIP2-4 and OsPIP2-5 increased in the roots, while at the same time it decreased in the leaves after 24 h of treatment with 10% PEG in rice [81]. The same profile of expression- increase in the roots and decrease in the shoots was detected for OsTIP1;1, OsTIP4;1 and OsTIP4;2 after 10 h of treatment with 15% PEG in rice [75]. The identification the AQP genes whose expression was inversely regulated in the shoots and roots may be a result of different roles that are played by specific AQP in the various organs in plants.
Aquaporins also facilitate transport substrates other than water such as hydrogen peroxide (H2O2) or glycerol, which both might be produced under drought stress. Hydrogen peroxide is unstable and highly reactive molecules, which belongs to the reactive oxygen species (ROS) [97]. In turn, glycerol is an osmotic compound [76,98]. The expression of HvTIP3;1 and HvTIP4;1 genes increased under drought stress in barley leaves [80]. This profile of expression was associated with the involvement of HvTIP3;1 in moving hydrogen peroxide. The transport of this substrate by this AQP was predicated in barley by bioinformatic analysis [64]. In barley, using the bioinformatic analysis the substrate other than water that can be transported by HvTIP4;1 has not been identified [64]. However, in rice it was demonstrated that its orthologue, OsTIP4;1, has a glycerol transport activity using the Xenopus oocyte expression system [75]. The increased expression of HvTIP4;1 under drought stress might also be connected with an increased transport of glycerol. In rice, a nine-fold increase in the expression of OsTIP4;1 was detected only in the shoots after 8 h of PEG-6000 treatment compared to the control conditions [75]. Other investigated durations of treatment of 2, 4, 6 and 10 h did not cause such big changes in the amount of the transcript [75].
Drought stress often occurs simultaneously with high temperatures in field conditions. Because of global climate change, temperature stress is becoming a major area of concern for researchers around the world [99]. Foxtail millet (Setaria italica L.) is a model plant for studying biofuels, stress tolerance and C4 photosynthetic traits [66]. The profile of expression of the aquaporin genes during heat stress in this species was investigated in the roots. After 24 h of treatment at 45 °C, all of the analysed genes, which are members of PIP, TIP, NIP and SIP subfamilies, had an increase in the abundance of these transcripts (Table 2) [66]. This might suggest that an increased uptake of water by the roots is needed to cool the temperature of leaves by increasing transpiration.

3.2. Cold Stress

Plants are exposed to a wide range of environmental conditions, and in addition to heat, cold stress is also a factor that shapes the physiology of cereals [99]. Low soil temperatures inhibit water uptake by roots [100]. A decrease in water permeability across the root (i.e., a decrease in the root hydraulic conductivity, Lpr) is expected to affect the plant water status, stomatal conductance, photosynthesis, growth and, lastly, productivity [94,101,102,103,104]. In rice, a decrease in the Lpr under cold stress was linked to the role of aquaporins [105]. A 96-h treatment at 4 °C was enough to cause a decrease in the expression of eight PIP and two TIP genes in rice (Table 2) [63]. Interestingly, the differences in the profiles of the AQP genes (nine PIPs and three TIPs) expression was also detected in rice when whole plants were chilled compared to when only the roots of the plants were chilled at 10 °C for five days. When only the roots were chilled, there was an increased expression of OsPIP1;3, OsPIP2;4 and OsPIP2;5 compared to the control conditions (Table 2) [79]. The authors observed that a low room temperature treatment (LRT) for a prolonged length of time induced a gradual increase the root osmotic hydraulic conductivity Lpr(os), which suggests that LRT-treated roots acclimate to low temperatures by acquiring a water uptake mechanism that is not present at the normal growth temperature of 25 °C [79]. This might suggest that a cold acclimation process for the root water uptake functions in rice and that is possibly regulated via aquaporins [79]. Furthermore, the low temperature causes changes in the amount of AQP transcripts not only in roots but also in leaves. Recently, it was shown that temperature of growth was a significant factor influencing the level of accumulation of HvPIP1 transcript and protein in barley leaves. The level of accumulation of HvPIP1 transcript decreased at 5 °C (compared to 20 °C) but was higher at 27 °C than at 20 °C in the analysed genotypes [106].

3.3. Salinity

Soil salination and a high salt accumulation affect the growth, development, metabolism and yield of plants [107]. A wide range of physiological and biochemical alterations in plants is induced by salinity, which causes a lower water potential in the soil solution and ionic disequilibrium, as well as a higher accumulation of reactive oxygen species (ROS) [107]. This is why the salt stress tolerance trait has multi-component nature [108]. Among cereals, barley is highly tolerant for salinity, while wheat and maize display a high sensitivity [34]. AQPs could play a role in the mechanism by which plants cope with saline concentration because it leads to changes in the AQP gene expression profiles, which has been shown in many plant species including cereals (Table 2). In barley (Hordeum vulgare L.), foxtail millet (Setaria italica L.) and rice (Oryza sativa L.), different concentrations of NaCl from 150 till 250 mM were used for the salt treatment, as well as different durations of the treatment (Table 2). During the early research on barley, the opposite profile of the expression of the HvPIP2;1 gene was detected in the roots and shoots after 48 h of treatment with 200 mM NaCl, which decreased and increased compared to the control conditions, respectively (Table 2) [109]. The HvPIP2;1 protein was confirmed to be localised in the plasma membrane. The functional expression of this gene in Xenopus oocyte indicated that HvPIP2;1 has a water transport activity [83]. Other research on both barley and rice also showed that after 24 h of NaCl treatment, there was a decrease in the expression of many members of the PIP subfamily in the roots (Table 2) [81,84]. Severe salinity stress (200 mM NaCl) also significantly reduced the root hydraulic conductivity (Lpr) in rice [84]. However, under relatively mild salt stress (100 mM NaCl), there was only a moderate decrease in the Lpr with no significant difference in the HvPIP mRNA levels [84]. The biological significance of such a regulation may increase the prevention of an excessive water loss from cells during the early stages (the osmotic stress phase) of salinity stress, and the Lpr decrease might represent the mode of change in barley plants from the rapid growth stage, which requires a high water absorption to the protected stage, which requires less water uptake [84]. Conversely, under salinity stress some TIP genes were detected whose expression increased compared to the control conditions such as OsTIP1;1 and OsTIP1;2 (Table 2) [75]. OsTIP1;2 had a water and glycerol transport activity [58]. The upregulation of some of the OsTIPs genes might be connected with water transport from the vacuole to the cytoplasm to perform an osmotic adjustment [75].

3.4. Comparison between Genotypes, Which Are Characterised as Tolerant or Resistant to Specific Abiotic Stress

An extremely interesting aspect of an expression study of AQP genes under abiotic stress conditions is the comparison between the cultivars, which are characterised by the opposite phenotype in terms of their tolerance to specific abiotic stresses. This kind of research could lead to identifying the important players among AQPs in stress tolerance. The genes that would be identified in such a way could serve as potential candidates for further downstream characterisation studies in order to understand the molecular mechanisms that underlie abiotic stress tolerance. Two contrasting cultivars of the foxtail millet (Setaria italica L.), which is a naturally abiotic stress-tolerant crop, were investigated in terms of their abiotic stress tolerance. The study showed that SiPIP3;1 and SiSIP1;1 were differentially expressed in both of the cultivars in response to salinity, heat and dehydration stresses [66]. In the stress-tolerant cultivar, the expression of these genes in roots were higher compared to the resistant ones. Their role in providing salinity and dehydration stress tolerance was also validated via the heterologous overexpression of these genes in the S. cerevisiae system in which the overexpression lines were found to be tolerant to abiotic stresses [66]. In turn, in rice, the expression of the aquaporin RWC3 gene (PIP1) was investigated in upland and lowland rice. Upland rice is traditionally regarded as having drought avoidance, which is one of the mechanisms of drought resistance [110]. The expression of RWC3 mRNA was higher in the upland rice in the early response (up to four hours) to a 20% polyethylene glycol (PEG) 6000 treatment in the roots, whereas there were no significant changes in its expression in the lowland rice. The protein levels increased in the upland rice and decreased in the lowland rice after a ten-h water deficit. An analysis with the Xenopus oocyte expression system showed that RWC3 has a water transport activity. The authors suggested that a high level of RWC3 improves water uptake under a water deficit and therefore maintains the plant water balance [111]. Another interesting example of an AQP gene expression study in two inbred lines that contrast in their water use efficiency, root hydraulic conductivity (Lpr) and AQP contribution to the Lpr was performed on pearl millet (Pennisetum glaucum (L) R. Br.). This is a key cereal for food security in arid and semi-arid regions of the world, which is heat and drought adapted [112]. Increased water use efficiency (WUE) can alleviate drought stress [67]. The line with a lower WUE had a significantly higher AQP contribution to the Lpr and also had a higher PgPIP1-3 and PgPIP1-4 gene expression compared to the line with a higher WUE that was grown in hydroponic conditions. The authors suggested that a downregulation of the PgPIP genes might induce inhibition of transpiration and water savings. However, an investigation of the expression patterns of the AQP genes under abiotic stress conditions could be a very important subject for further research. Additionally, treating roots with a common AQP inhibitor (azide) suggested that AQP contributed up to 84% of the root hydraulic conductivity (Lpr) in pearl millet [67]. Furthermore, in the same cereal, four recombinant inbred lines that vary in their transpiration rate (Tr) response to vapour pressure deficit (VPD) conditions were selected for an expression study of the AQP genes [113]. Some of the water-saving traits were the capacity of certain genotypes to restrict plant transpiration when the evaporative requisition was high [114]. The differences in the transpiration response phenotype were accompanied by diverse AQP gene abundances between the VPD-sensitive and VPD-insensitive genotypes. The expression pattern of the PgAQP genes indicated that the VPD-insensitive genotypes had a slightly higher expression of AQPs, including PgPIP1;1 and PgPIP2;6 in the root tissue, than in the VPD-sensitive genotypes under low VPD conditions (1.2 kPa), when there was no high evaporative demand from the atmosphere [113]. The last example is from wheat (Triticum aestivum L.) for which the profiles of the AQP genes were investigated using accessions with contrasting responses to drought according to the yield tolerance index (YTI). In the leaves, as stress intensity increased, some of the AQP genes of the PIP1 and PIP2 subfamilies such as TaPIP1-1, TaPIP1-5 or TaPIP2-24 were clearly upregulated in the tolerant genotype. In the case of the TIP family, TaTIP2-4, TaTIP3-4 and TaTIP4-1 also increased their expression levels during severe stress. On the other hand, in the roots, the tolerant genotype had a lower level of expression compared to the susceptible ones for the TaPIP1-1, TaPIP1-5, TaAQP7, TaPIP2-1C1, TaTIP1-2, TaTIP3-4 and TaTIP4-1 genes under moderate stress [61].

4. Overexpression and Mutant Lines in the Cereal Aquaporin-Encoding Genes

The AQP genes are a potential target for crop improvement, which is primarily connected with their functions in regulating the water balance in plants. Only members of the plasma membrane intrinsic proteins (PIP) and nodulin-26-like proteins (NIP) family genes from cereals have been reported to be involved in increasing the tolerance to abiotic stress in several plant species using genetic modification technology (Table 3). With the most common plants, the goal of the research was to manipulate the AQP gene expression to increase the tolerance to either drought or salt stress or both. Genetically manipulating the cereal AQP genes that belong to the PIP1 group such as RWC3, OsPIP1;1, OsPIP1;2 and TaAQP8 was performed and transgenic plants were generated in rice, Arabidopsis and tobacco [78,81,111,115,116]. In turn, in the PIP2 group, PsPIP2;2, TaAQP7, TdPIP2;1 and ZmPIP2;5, were investigated and transgenic plants were created in Arabidopsis, tobacco, wheat and maize [81,117,118,119]. An increased drought or salt stress tolerance of transgenic plants was reported based on an increase in some parameters after growth in stress conditions such as the germination rate, root growth, biomass production, root osmotic hydraulic conductivity (Lp), relative water potential (RWC) in the leaves, transpiration, leaf elongation rate, retained low Na+ and high K+ concentrations in the shoots, a lower H2O2 accumulation and less membrane damage because the antioxidant system was improved (Table 3). Maintaining transpiration is important in plants, especially during drought stress, because it facilitates the dissipation of excess heat. This effect was obtained by the overexpression of Oryza sativa RWC3 in rice, and the relative cumulative transpiration after ten hours of treatment with 20% PEG 6000 was higher compared to the wild type plants (WT) [111]. Interestingly, the transgenic plants with an overexpression of Hordeum vulgare HvPIP2;5 in Arabidopsis were able to survive and recover after a three-week period of drought [120]. Manipulating the expression of the AQP genes does not always lead to the intended results. There is one example in which the overexpression of the Hordeum vulgare HvPIP2;1 gene resulted in an increased sensitivity to abiotic stress. Transgenic rice plants with an overexpression of this gene were more sensitive to 100 mM NaCl [109]. In turn, the overexpression of OsPIP1;2 in rice improved not only growth, but importantly also grain yield by facilitating leaf CO2 diffusion, which increases both the net CO2 assimilation rate and sucrose transport [78]. Not only were plants with an overexpression of some AQP genes created, but those with the knockout of AQP gene were also investigated in order to improve the plant response to abiotic stress. Rice is a major source of toxic metalloid arsenic in food. The arsenic accumulation in rice grains leads to food contamination. The knockout of either the Oryza sativa OsNIP1;1 or OsNP3;3 gene had little effect on arsenite uptake or translocation. However, the overexpression of the genes did decrease the root-to-shoot translocation of arsenite and the shoot arsenic concentration by disrupting the radial transport of arsenite in the roots. When they were grown in arsenic-contaminated paddy soils, the transgenic plants contained significantly lower arsenic concentrations in their rice grains [121].

5. Concluding Remarks

According to the Intergovernmental Panel on Climate Change report, the global mean temperature increased by 0.8 °C in the 20th century and is predicted to increase by 3–5 °C in the 21st century [122]. This means that extreme daily temperatures and heat waves might be a growing problem for sustainable agriculture in the future. Not only increases in temperature but also predicted increases in soil salinisation and levels of CO2 in the atmosphere will effect cereal growth and yield [123,124].
Water is a fundamental compound for living all organisms. It is believed that aquaporins may be important players in the plant water relations at the cell, tissue, organ and whole plant levels. AQPs may fine-tune the water transport through its transcriptional regulation and establish a suitable water balance under stress conditions. Therefore, these proteins may play a crucial role in the stress response as well as in stress tolerance and might also serve as a target sequence for genetic modifications.
Different studies have associated the changes in the AQPs profiles of expression with an adaptive mechanism that limits the effects of different abiotic stresses. A functional analysis of these candidate genes or proteins will be necessary to draw adequate conclusions. Taken together, AQPs probably play complex and diversified roles in the response of plants to abiotic stresses that are dependent on specific isoforms and the type and degree of stress treatment. In order to improve the tolerance of cereals to abiotic stress conditions, a better understanding of the AQP family members and their functions in plant physiology is needed.

Funding

This research was funded by the National Science Centre, Poland, SONATA project 2015/19/D/NZ1/03441.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The study did not report any data.

Conflicts of Interest

The author declares no conflict of interest.

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Genes 12 00477 g001 550
Figure 1. Transmembrane helix prediction for the HvTIP2;3 protein sequence of barley (HORVU7Hr1G081770) that was obtained using Phyre2 software [14]. S1–S6: six transmembrane helices, A–E: five connecting inter-helical loops.
Figure 1. Transmembrane helix prediction for the HvTIP2;3 protein sequence of barley (HORVU7Hr1G081770) that was obtained using Phyre2 software [14]. S1–S6: six transmembrane helices, A–E: five connecting inter-helical loops.
Genes 12 00477 g001
Genes 12 00477 g002 550
Figure 2. The structure of aquaporin represented by the crystal structure of Arabidopsis AtPIP2;4 (PDB 6QIM) [20]. The view from different sides of the holoprotein shows that it consists of monomers. (a) A side view of an asymmetric unit. (b) Tetrameric assembly from the cytoplasmic side.
Figure 2. The structure of aquaporin represented by the crystal structure of Arabidopsis AtPIP2;4 (PDB 6QIM) [20]. The view from different sides of the holoprotein shows that it consists of monomers. (a) A side view of an asymmetric unit. (b) Tetrameric assembly from the cytoplasmic side.
Genes 12 00477 g002
Table
Table 1. Number of aquaporin (AQP) isoforms in the genome of cereal species and its area harvested.
Table 1. Number of aquaporin (AQP) isoforms in the genome of cereal species and its area harvested.
SpeciesArea Harvested (ha) [60]Genome Size/Ploidy x/
No. of Chromosomes		AQPPIPTIPNIPSIPReferences
Bread wheat
(Triticum aestivum L.)		215,901,958~17,000 Mb
2n = 6x = 42 AABBDD hexaploid		113
(65-A, 42-B, 36-D)		5129294[61]
Maize
(Zea mays L.)		197,204,2502400 Mb
2n = 2x = 20		41121883[62]
Rice Japonica
(Oryza sativa)		162,055,938500 Mb
2n = 2x = 24		331110102[63]
Barley
(Hordeum vulgare L.)		51,149,869~5300 Mb
2n = 2x = 14		39181182[64]
Sorghum
(Sorghum bicolor (L.) Moench)		40,074,667~730 Mb
2n = 2x = 20		371311112[65]
Foxtail millet
(Setaria italica)		31,653,878 ^~490 Mb
2n = 2x = 18		391211133[66]
Pearl millet (Pennisetum glaucum (L.) R Br.)31,653,878 ^~1790 Mb
2n = 2x = 14		33109113[67]
^ both harvested millet areas together.
Table
Table 2. Study of the expression the AQP genes in cereal species under abiotic stress.
Table 2. Study of the expression the AQP genes in cereal species under abiotic stress.
Abiotic StressCereal SpeciesStage of GrowthTreatmentMethodAnalysed AQP
Genes		Effect on the Expression Level/TissueReferences
IncreasedDecreased
ColdRice (Oryza sativa L. spp. japonica)16-week-old
seedlings		4 °C treatment,
96 h		RT-PCR **8 PIPs
2 TIPs		 Roots:
OsPIP1;1, OsPIP1;2, OsPIP2;1, OsPIP2;2,
OsPIP2;3, OsPIP2;4, OsPIP2;5, OsPIP2;6, OsTIP1;1
OsTIP2;2		[63]
Rice
(Oryza sativa L. spp. japonica)		16–20-day-old seedlings Root chilling: culture solution set in a water bath maintained at °C;
whole-plant chilling: plants were placed in a 10 °C set
growth chamber,
5 days ^		qRT-PCR *9 PIPs
3 TIPs		Roots chilled:
OsPIP1;3
OsPIP2;4 OsPIP2;5		Roots chilled:
OsPIP2;6
OsTIP1;1
OsTIP2;2
Whole plant chillled:
all analysed		[79]
Drought/dehydratationBarley (Hordeum vulgare L.)24-day-old seedlingsten days of drought in soil under a volumetric water content of 1.5%qRT-PCR *8 TIPsLeaves:
HvTIP3;1 HvTIP4;1		Leaves:
HvTIP1;1
HvTIP1;2
HvTIP2;1
HvTIP2;2
HvTIP2;3		[80]
Foxtail millet
(Setaria italica L.),
tolerant cultivar		21-day-old seedlingsCultivated with
20% PEG 6000,
24 h ^		qRT-PCR *2 PIPs
1 TIP
1 NIP
1 SIP		Roots:
SiPIP3;1
SiSIP1;1
SiTIP2;2		Roots:
SiPIP1;2
SiNIP1;2		[66]
Rice (Oryza sativa L. spp. japonica)two-week-old
seedlings		Cultivated with
10% PEG 6000,
24 h ^		qRT-PCR *10 PIPsRoots:
OsPIP1-1
OsPIP1-2
OsPIP1-3
OsPIP2-4
OsPIP2-5
OsPIP2-7		Leaves:
OsPIP2-1
OsPIP2-4
OsPIP2-5
OsPIP2-6		[81]
Rice (Oryza sativa L. spp. japonica)four-week-old
seedlings		Cultivated hydroponically with
15% PEG 6000,
10 h ^		qRT-PCR *7 TIPsRoot:
OsTIP1;1
OsTIP1;2
OsTIP4;1
OsTIP4;2		Shoot:
OsTIP1;1
OsTIP2;2
OsTIP4;1
OsTIP4;2		[75]
Rice (Oryza sativa L.), 6 rice varieties29-day-old seedlingsDrought in soilqRT-PCR * in Azucena variety (japonica group) ^10 PIPs Roots:
All analysed		[82]
Wheat (Triticum aestivum L.)one-week-old seedlingscultivated with
20% PEG-6000,
6 h ^		Microarray data obtained from WheatExp
database		113 AQPs17 PIPs
12 TIPs
Examples *:
TaTIP1-2
TaTIP1-4
TaTIP2-3
TaTIP2-4
TaTIP3-3
TaTIP3-4
TaTIP3-6
TaTIP4-1
TaTIP4-4
TaTIP4-5		16 PIPs
3 SIPs
Examples *:
TaNIP1-3 TaNIP1-4
TaNIP1-6 TaNIP2-1C1
TaTIP2-2 TaTIP4-1C1
TaPIP2-1
TaPIP2-3
TaPIP2-4C1
TaPIP2-6
TaPIP2-10
TaPIP2-12
TaPIP2-14
TaPIP2-19		[61]
Wheat (Triticum aestivum L.),
drought-tolerant genotype compared to the control conditions		Anthesis stage (Z61) —grain fillingDrought stress—without irrigationqRT-PCR *8 PIPs
4 TIPs
2 NIPs		Leaves:
TaPIP1-1 TaPIP1-5 TaPIP2-24
Roots:
TaPIP1-1
TaPIP1-5
TaPIP2-2C1
TaTIP1-2
TaTIP2-4
TaTIP3-4
TaNIP4-3
TaLEA		Leaves:
TaNIP4-2
Roots:
TaPIP1-6 TaPIP2-7
TaPIP2-24
TaNIP4-1		[61]
HeatFoxtail millet
(Setaria italica L.),
tolerant cultivar		21-day-old seedlings45 °C treatment,
24 h		qRT-PCR *2 PIPs
1 TIP
1 NIP
1 SIP		Roots:
SiPIP1;2
SiPIP3;1
SiSIP1;1
SiTIP2;2
SiNIP1;2		 [66]
SalinityBarley (Hordeum vulgare L.)four-day-old
seedlings		cultivated with
200 mM NaCl,
48 h		RT-PCR **3 PIPsShoots:
HvPIP2;1		Roots:
HvPIP2;1		[83]
Barley (Hordeum vulgare L.)four-day-old
seedlings		cultivated with
200 mM NaCl,
24 h		qRT-PCR *10 PIPs Roots:
HvPIP1;2, HvPIP1;3, HvPIP1;4, HvPIP2;1,
HvPIP2;2 HvPIP2;3		[84]
Foxtail millet
(Setaria italica L.),
tolerant cultivar		21-day-old seedlingscultivated with
200 mM NaCl,
24 h		qRT-PCR *2 PIPs
1 TIP
1 NIP
1 SIP		Roots:
SiPIP1;2
SiPIP3;1
SiSIP1;1
SiTIP2;2		Roots:
SiNIP1;2		[66]
Rice (Oryza sativa)two-week-old
seedlings		cultivated with
250 mM NaCl,
24 h ^		qRT-PCR *10 PIPsLeaves:
OsPIP1-2
Roots:
OsPIP1-1
OsPIP1-2
OsPIP2-4		Leaves:
OsPIP1-1
OsPIP2-1
OsPIP2-3
OsPIP2-4
OsPIP2-6
Roots:
OsPIP1-3
OsPIP2-2
OsPIP2-3
OsPIP2-5		[81]
Rice (Oryza sativa L. spp. japonica)four-week-old
seedlings		cultivated hydroponically with
150 mM NaCl,
10 h ^		qRT-PCR *7 TIPsRoots:
OsTIP1;1
OsTIP1;2
OsTIP4;1
OsTIP4;2		Shoots:
OsTIP1;2
OsTIP2;2
OsTIP4;2
OsTIP4;3
Roots:
OsTIP2;2
OsTIP4;3		[75]
* Quantitative real time PCR (qRT-PCR); ** semi-quantitative RT-PCR, end-point technique; ^ the time of treatment that was selected; treatment with PEG 6000 was used to mimic the drought stress.
Table
Table 3. Genetic manipulation of the AQP genes of cereals and its effect on the plant phenotype.
Table 3. Genetic manipulation of the AQP genes of cereals and its effect on the plant phenotype.
SpeciesAQP Gene Method/Expression in SpeciesPromoterImproved Tolerance to Abiotic Stress/or Other TraitsPhenotypeReferences
Hordeum vulgare L.HvPIP2;1OX
Oryza sativa		CaMV35SNoIn control conditions, there was an increased radial hydraulic conductivity of roots (Lpr) of up to 140% and the mass ratio of the shoot to root of up to 150%. Under salt stress of 100 mM NaCl, reduction in growth was greater than in non-transgenic plants[109]
HvPIP2;5OX
Arabidopsis thaliana		CaMV35SYesBetter stress tolerance during germination and root growth under high salt and high osmotic stresses. Able to survive and recover after a three-week drought[120]
Oryza sativa L.RWC3
(PIP1 group)		OX
Oryza sativa, lowland		SWPA2YesBetter water status under a water deficit. Increased root osmotic hydraulic conductivity (Lp), leaf water potential and relative cumulative transpiration at the end of ten-h treatment with 20% PEG 6000[111]
OsPIP1;1OX
Oryza sativa		CaMV35SYesIncreased seed yield, salt resistance, root hydraulic conductivity and seed germination rate[115]
OsPIP1;1
OsPIP2;2		OX
Arabidopsis thaliana		CaMV35SYesImproved tolerance to salt (100 mM of NaCl) and drought (200 mM of mannitol), but not to salt treatment at a higher concentration (150 mM of NaCl)[81]
OSPIP1;2OX
Oryza sativa		CaMV35SYesImproved growth and grain yield by
facilitating leaf CO2 diffusion, which increases both the net CO2 assimilation rate and sucrose transport.		[78]
OsNIP1;1,
OsNIP3;3		OX
KO
Oryza sativa		OsLsi1YesKnockout of either gene had little effect on arsenite uptake or translocation. Overexpression did not affect arsenite uptake but decreased the root-to-shoot translocation of arsenite and shoot arsenic concentration. When grown in arsenic-contaminated paddy soils, there was a significantly lower arsenic concentration in the rice grains[121]
Triticum aestivum/
turgidum L.		TaAQP7
(PIP2 group)		OX
Nicotiana tabacum		CaMV35SYesIncreased drought tolerance. Lower levels of malondialdehyde (MDA) and H2O2 and less ion leakage (IL), but a higher relative water content (RWC) and superoxide dismutase (SOD) and catalase (CAT) activities[117]
TaAQP8
(PIP1 group)		OX
Nicotiana tabacum		CaMV35SYesIncreased root elongation compared to the controls under salt stress. Retaining a high K+/Na+ ratio and Ca2+ content, but also lowering the H2O2 accumulation and membrane damage by improving the antioxidant system[116]
TdPIP2;1OX
Triticum durum		PrCaMV35SYesImproved germination rates and biomass production and retained low Na+ and high K+ concentrations in the shoots under high salt and osmotic stress conditions. A long-term study under greenhouse conditions on salt or drought stress produced good quality grains[118]
Zea mays L.ZmPIP2;5OX
KO
Zea mays		CaMV35SYesWhole-root conductivity decreased in the KO lines; no difference was observed in the OX plants. At the leaf level, the hydraulic conductance was higher in the PIP2;5 OE lines whereas there was no difference in the pip2;5 KO lines. Leaf elongation rate was faster in the PIP2;5 OE plants after mild drought stress[119]
OX—overexpression, KO—knockout, PEG—polyethylene glycol, CaMV35S—the cauliflower mosaic virus p35S promoter.
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Kurowska, M.M. Aquaporins in Cereals—Important Players in Maintaining Cell Homeostasis under Abiotic Stress. Genes 2021, 12, 477. https://doi.org/10.3390/genes12040477

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Kurowska MM. Aquaporins in Cereals—Important Players in Maintaining Cell Homeostasis under Abiotic Stress. Genes. 2021; 12(4):477. https://doi.org/10.3390/genes12040477

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Kurowska, Marzena Małgorzata. 2021. "Aquaporins in Cereals—Important Players in Maintaining Cell Homeostasis under Abiotic Stress" Genes 12, no. 4: 477. https://doi.org/10.3390/genes12040477

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Kurowska, M. M. (2021). Aquaporins in Cereals—Important Players in Maintaining Cell Homeostasis under Abiotic Stress. Genes, 12(4), 477. https://doi.org/10.3390/genes12040477

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