Potential of Alpha-(α)-Solanine as a Natural Inhibitor of Fungus Causing Leaf Spot Disease in Strawberry
Abstract
:1. Introduction
2. Materials and Methods
2.1. Source of Fungi and α-Solanine
2.2. RNA Extraction and Illumina Sequencing
2.3. Sequence Analysis and Mapping
2.4. Gene Annotation, Enrichment, and Expression Analysis
2.5. Metabolome Analysis of Curvularia
3. Results
3.1. Effects of α-Solanine on Spore Germination
3.2. Evaluation of Sequence Reads and Features
3.3. Gene Annotation of Curvularia trifolii
3.4. Effect of α-Solanine Treatment on the Curvularia Transcriptome
3.5. COG, KEGG, and GO Enrichment and Annotations for α-Solanine Responsive DEGs
3.6. Effect of α-Solanine on the Metabolites of Curvularia
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sample ID | A1 | A2 | A3 | B1 | B2 | B3 |
---|---|---|---|---|---|---|
Number of reads (Millions) | 42 | 48 | 44 | 44 | 44 | 48 |
Average read size | 144 | 142 | 146 | 143 | 145 | 142 |
Total length | 6,081,757,990 | 6,900,038,202 | 6,453,851,237 | 6,394,567,695 | 6,425,063,616 | 6,929,690,377 |
GC% | 52.56 | 52.36 | 53.26 | 51.56 | 52.3 | 52.21 |
Q30% | 92 | 92.18 | 91.72 | 91.9 | 92.31 | 92.32 |
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Xu, N.; Lu, H.; Yi, X.; Peng, S.; Huang, X.; Zhang, Y.; He, C. Potential of Alpha-(α)-Solanine as a Natural Inhibitor of Fungus Causing Leaf Spot Disease in Strawberry. Life 2023, 13, 450. https://doi.org/10.3390/life13020450
Xu N, Lu H, Yi X, Peng S, Huang X, Zhang Y, He C. Potential of Alpha-(α)-Solanine as a Natural Inhibitor of Fungus Causing Leaf Spot Disease in Strawberry. Life. 2023; 13(2):450. https://doi.org/10.3390/life13020450
Chicago/Turabian StyleXu, Ning, Huan Lu, Xueqian Yi, Simin Peng, Xiaohui Huang, Yu Zhang, and Changzheng He. 2023. "Potential of Alpha-(α)-Solanine as a Natural Inhibitor of Fungus Causing Leaf Spot Disease in Strawberry" Life 13, no. 2: 450. https://doi.org/10.3390/life13020450