Transcriptional Profiles of Mitochondrial Dynamics Markers Are Disturbed in Adrenal Glands of Stressed Adult Male Rats
Abstract
:1. Introduction
2. Materials and Methods
2.1. Statement of Institutional Review Board
2.2. A Statement That the Authors Complied with ARRIVE Guidelines and Institutional Animal Care and Use Committee Guidelines
2.3. Animals and Experimental Model of Stress
2.4. Isolation of Adrenal Glands
2.5. Isolation of RNA and cDNA Synthesis
2.6. Relative Quantification of Gene Expression
2.7. Relative Quantification of Protein Expression
2.8. Statistical Analysis
3. Results
3.1. The Transcriptional Profiles of Mitochondrial Biogenesis Markers Were Changed in the Adrenal Gland of Stressed Rats
3.2. Repeated Psychophysical Stress Increased the Transcriptional Profile of Mitochondrial Fusion and Architecture Markers in the Adrenal Gland
3.3. The Transcriptional Profiles of Mitochondrial Fission Markers Were Increased in the Adrenal Gland after Ten Repeated Stress Incidents
3.4. Ten Repeated Psychophysical Stress Incidents Increased the Transcriptional Profile of Mitochondrial Autophagy Markers in the Adrenal Gland
3.5. The Transcription of Mitochondrial Functionality Markers Was Changed in the Adrenal Gland of Stressed Rats
3.6. The Transcription of Adrenergic Receptors, Adrenergic Receptor Kinases, and Glucocorticoid Receptor Was Changed in the Adrenal Gland of Stressed Rats
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Keselj, I.M.; Bozic, F.N.; Vucinic, M.M.; Lalosevic, D.; Kostic, T.S.; Andric, S.A. Transcriptional Profiles of Mitochondrial Dynamics Markers Are Disturbed in Adrenal Glands of Stressed Adult Male Rats. Life 2023, 13, 1457. https://doi.org/10.3390/life13071457
Keselj IM, Bozic FN, Vucinic MM, Lalosevic D, Kostic TS, Andric SA. Transcriptional Profiles of Mitochondrial Dynamics Markers Are Disturbed in Adrenal Glands of Stressed Adult Male Rats. Life. 2023; 13(7):1457. https://doi.org/10.3390/life13071457
Chicago/Turabian StyleKeselj, Isidora M., Filip N. Bozic, Miodrag M. Vucinic, Dusan Lalosevic, Tatjana S. Kostic, and Silvana A. Andric. 2023. "Transcriptional Profiles of Mitochondrial Dynamics Markers Are Disturbed in Adrenal Glands of Stressed Adult Male Rats" Life 13, no. 7: 1457. https://doi.org/10.3390/life13071457