Identification of Fusarium oxysporum Causing Leaf Blight on Dendrobium chrysotoxum in Yunnan Province, China
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. Isolation of the Pathogen
2.3. Morphological Identification
2.4. DNA Extraction
2.5. PCR Amplification, Molecular Identification, and Construction of Phylogenetic Tree
2.6. Pathogenicity Test
2.7. Pathogen Reisolation
3. Results
3.1. Nursery Observations and Isolations
3.2. Morphological Identification
3.3. Molecular Identification
3.4. Pathogenicity Test
3.5. Pathogen Reisolation
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Primers | Sequence (5′–3′) | Annealing Temperature (°C) |
---|---|---|---|
ITS | ITS1-F | TCCGTAGGTGAACCTGCGG | 55 |
ITS4-R | TCCTCCGCTTATTGATATGC | ||
EF-1α | EF1-F | ATGGGTAAGGARGACAAGAC | 55 |
EF2-R | GGARGTACCAGTSATCATG | ||
RPB1 | F7-F | CRACACAGAAGAGTTTGAAGG | 55 |
R8-R | CAATGAGACCTTCTCGACCAGC | ||
RPB2 | 5F2-F | GGGGWGAYCAGAAGAAGGC | 55 |
7CR-R | CCCATRGCTTGYTTRCCCAT |
Isolate | Fusarium MLST (Number) | Fusarium MLST Similarity % | GenBank Accession Number | |||
---|---|---|---|---|---|---|
ITS | EF-1α | RPB1 | RPB2 | |||
DHRL-01 | Fusarium oxysporum species complex (NRRL 22549) | 100 | MW599746 | MW703468 | MW703472 | MW703476 |
DHRL-02 | Fusarium oxysporum species complex (NRRL 22549) | 100 | MW599747 | MW703469 | MW703473 | MW703477 |
DHRL-03 | Fusarium oxysporum species complex (NRRL 22549) | 100 | MW599748 | MW703470 | MW703474 | MW703478 |
DHRL-04 | Fusarium oxysporum species complex (NRRL 22549) | 100 | MW599749 | MW703471 | MW703475 | MW703479 |
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Yang, J.; Ahmed, W.; Zhang, J.; Gao, S.; Wang, Z.; Yang, H.; Bai, X.; Luo, K.; Xu, C.; Ji, G. Identification of Fusarium oxysporum Causing Leaf Blight on Dendrobium chrysotoxum in Yunnan Province, China. Life 2024, 14, 285. https://doi.org/10.3390/life14030285
Yang J, Ahmed W, Zhang J, Gao S, Wang Z, Yang H, Bai X, Luo K, Xu C, Ji G. Identification of Fusarium oxysporum Causing Leaf Blight on Dendrobium chrysotoxum in Yunnan Province, China. Life. 2024; 14(3):285. https://doi.org/10.3390/life14030285
Chicago/Turabian StyleYang, Jun, Waqar Ahmed, Jinhao Zhang, Shunyu Gao, Zhenji Wang, Haiyan Yang, Xuehui Bai, Kai Luo, Chengdong Xu, and Guanghai Ji. 2024. "Identification of Fusarium oxysporum Causing Leaf Blight on Dendrobium chrysotoxum in Yunnan Province, China" Life 14, no. 3: 285. https://doi.org/10.3390/life14030285