Evodiamine Alleviates 2,4-Dinitro-1-Chloro-Benzene-Induced Atopic Dermatitis-like Symptoms in BALB/c Mice
Abstract
:1. Introduction
2. Results
2.1. Evodiamine Suppresses Atopic Dermatitis Symptoms in the Ears of Mice
2.2. Evodiamine Suppresses Pro-Inflammatory Cytokine Expressions in the Ears of Mice
2.3. Evodiamine Suppresses DNCB-Induced Immune Responses in Lymph Nodes
2.4. Evodiamine Suppresses Pro-Inflammatory Cytokine Expressions in the Ears of Mice
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Mouse Strain
4.3. Induction of DNCB-Induced Atopic Dermatitis in BALB/c Mice
4.4. Total Immunoglobulin E (IgE) Levels in Serum
4.5. Mast Cell Count in the Skin
4.6. Histologic Analysis and Measurement of Ear Thickness
4.7. Quantitative Real-Time PCR
4.8. Statistics
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Mouse Primers | Sequence | |
---|---|---|
Il-6 | forward | 5′-TTC TTG GGA CTG ATG CTG GT-3′ |
reverse | 5′-CTG TGA AGT CTC CTC TCC GG-3′ | |
Il-8 | forward | 5′-AAC TCC TTG GTG ATG CTG GT-3′ |
reverse | 5′-CCA GGT TCA GCA GGT AGA CA-3′ | |
Il-12A | forward | 5′-GAA GCT CTG CAT CCT GCT TC-3′ |
reverse | 5′-CAG ATA GCC CAT CAC CCT GT-3′ | |
IFN-γ | forward | 5′-CAC GGC ACA GTC ATT GAA AG-3′ |
reverse | 5′-GTC ACC ATC CTT TTG CCA GT-3′ | |
Il-4 | forward | 5′-TCT CGA ATG TAC CAG GAG CC-3′ |
reverse | 5′-CCT TCT CCT GTG ACC TCG TT-3′ | |
Il-13 | forward | 5′-GCA GCA TGG TAT GGA GTG TG-3′ |
reverse | 5′-AGG CCA TGC AAT ATC CTC TG-3′ | |
Il-22 | forward | 5′-GTC AAC CGC ACC TTT ATG CT-3′ |
reverse | 5′-GTT GAG CAC CTG CTT CAT CA-3′ | |
Il-17A | forward | 5′-TCC AGC AAG AGA TCC TGG TC-3′ |
reverse | 5′-AGC ATC TTC TCG ACC CTG AA-3′ | |
Gapdh | forward | 5′-AGA ACA TCA TCC CTG CAT CC-3′ |
reverse | 5′-CAC ATT GGG GGT AGG AAC AC-3′ |
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Han, S.-Y.; Im, D.-S. Evodiamine Alleviates 2,4-Dinitro-1-Chloro-Benzene-Induced Atopic Dermatitis-like Symptoms in BALB/c Mice. Life 2024, 14, 494. https://doi.org/10.3390/life14040494
Han S-Y, Im D-S. Evodiamine Alleviates 2,4-Dinitro-1-Chloro-Benzene-Induced Atopic Dermatitis-like Symptoms in BALB/c Mice. Life. 2024; 14(4):494. https://doi.org/10.3390/life14040494
Chicago/Turabian StyleHan, So-Young, and Dong-Soon Im. 2024. "Evodiamine Alleviates 2,4-Dinitro-1-Chloro-Benzene-Induced Atopic Dermatitis-like Symptoms in BALB/c Mice" Life 14, no. 4: 494. https://doi.org/10.3390/life14040494