Serological Tests in the Detection of SARS-CoV-2 Antibodies
Abstract
:1. Introduction
2. Methods
2.1. ELISAs
2.2. Lateral Flow Assay (LFA)
2.3. Statistical Analysis
3. Results
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Test | Anti-SARS-CoV-2 Antibodies Isotype | Serological Assays | Antigenic Region of the RBD Protein |
---|---|---|---|
MAGLUMI | IgG/IgM | ELISA | N and S |
EUROIMMUN | IgG/IgA | ELISA | S (S1) |
Dia.Pro | IgG/IgM | ELISA | N and S |
The Binding Site Group Ltd. | IgG/IgA/IgM | ELISA | S (Trimer) |
The Orient Gene Biotech | IgG/IgM | LFA | N |
Tests | Samples | Positive (%) | Negative (%) | Total (%) | ||||
---|---|---|---|---|---|---|---|---|
Concordance | ||||||||
Dia.Pro | Binding-Site | Euroimmun | Maglumi | OrientGene | 45 | 55.55 | 4.44 | 60.00 |
Dia.Pro | Binding-Site | Euroimmun | 132 | 38.63 | 33.33 | 71.97 | ||
Dia.Pro | Binding-Site | 171 | 36.84 | 38.59 | 75.44 | |||
Dia.Pro | Euroimmun | 132 | 42.42 | 33.33 | 75.76 | |||
Binding-Site | Euroimmun | 220 | 29.54 | 15.00 | 44.55 | |||
Dia.Pro | Maglumi | 45 | 66.66 | 4.44 | 71.11 | |||
Dia.Pro | OrientGene | 45 | 77.77 | 4.44 | 82.22 |
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Guevara-Hoyer, K.; Fuentes-Antrás, J.; De la Fuente-Muñoz, E.; Rodríguez de la Peña, A.; Viñuela, M.; Cabello-Clotet, N.; Estrada, V.; Culebras, E.; Delgado-Iribarren, A.; Martínez-Novillo, M.; et al. Serological Tests in the Detection of SARS-CoV-2 Antibodies. Diagnostics 2021, 11, 678. https://doi.org/10.3390/diagnostics11040678
Guevara-Hoyer K, Fuentes-Antrás J, De la Fuente-Muñoz E, Rodríguez de la Peña A, Viñuela M, Cabello-Clotet N, Estrada V, Culebras E, Delgado-Iribarren A, Martínez-Novillo M, et al. Serological Tests in the Detection of SARS-CoV-2 Antibodies. Diagnostics. 2021; 11(4):678. https://doi.org/10.3390/diagnostics11040678
Chicago/Turabian StyleGuevara-Hoyer, Kissy, Jesús Fuentes-Antrás, Eduardo De la Fuente-Muñoz, Antonia Rodríguez de la Peña, Marcos Viñuela, Noemí Cabello-Clotet, Vicente Estrada, Esther Culebras, Alberto Delgado-Iribarren, Mercedes Martínez-Novillo, and et al. 2021. "Serological Tests in the Detection of SARS-CoV-2 Antibodies" Diagnostics 11, no. 4: 678. https://doi.org/10.3390/diagnostics11040678