An Emerging Anti-p16 Antibody-BC42 Clone as an Alternative to the Current E6H4 for Use in the Female Genital Tract Pathological Diagnosis: Our Experience and a Review on p16ink4a Functional Significance, Role in Daily-Practice Diagnosis, Prognostic Potential, and Technical Pitfalls
Abstract
:1. Introduction
2. Applications of p16 in Daily-Practice Gynecologic Pathology
2.1. Vulva
2.2. Cervical Squamous Lesions
2.3. Glandular Lesions of Uterine Cervix and Endometrium
2.4. Mesenchymal Tumors of the Female Genital Tract
2.5. Ovary
3. Materials and Methods
3.1. Ethic Statement and Patient Selection
3.2. Pathological Assessment and Immunohistochemistry (IHC)
4. Results
4.1. Neoplastic Lesions of the Uterine Cervix
4.2. Neoplastic Lesions of the Uterine Corpus
4.3. p16 Expression in High-Grade Ovarian Cancer
5. Discussion
- -
- Pre-analytical variables: Specimen collection and handling, tissue fixation (uniformity, time, and type), and protocol of processing
- -
- Analytical variables: Choice of immunohistochemistry protocol, reagent variability, antigen retrieval technique, and technician training/expertise
- -
- Post-analytical variables: Evaluation of positive/negative controls, morphological correlations, diagnostic and prognostic significance, correlation with other data, interpretation, and reporting of results, and experience/expertise of the pathologist
6. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Tissue | Total Cases | BC42+ | % Positive | E6H4+ | % Positive |
---|---|---|---|---|---|
H-SIL | 36 | 35 | 97.22 | 35 | 97.22 |
-CIN3 | 18 | 18 | 100 | 18 | 100 |
-CIN2 | 18 | 17 | 94.44 | 17 | 94.44 |
Endocervical Usual type Adenocarcinoma | 30 | 30 | 100 | 30 | 100 |
Cervical Squamous cell carcinoma | 22 | 22 | 100 | 22 | 100 |
Endometrial Carcinoma | 70 | 70 | 100 | 70 | 100 |
-endometrioid type | 40 | 32 | 80 focal/patchy | 32 | 80 focal/patchy |
8 | 20 diffuse | 8 | 20 diffuse | ||
-serous type | 30 | 30 | 100 | 30 | 100 |
Ovarian High-grade Serous Carcinoma | 25 | 21 | 84 | 20 | 80 |
Uterine Adenomatoid Tumor | 6 | 6 | 100 | 6 | 100 |
Uterine Leyomiosarcoma | 10 | 6 | 60 | 7 | 70 |
4 diffuse | 5 diffuse | ||||
2 focal | 2 focal |
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Angelico, G.; Santoro, A.; Inzani, F.; Straccia, P.; Spadola, S.; Arciuolo, D.; Valente, M.; D’Alessandris, N.; Benvenuto, R.; Travaglino, A.; et al. An Emerging Anti-p16 Antibody-BC42 Clone as an Alternative to the Current E6H4 for Use in the Female Genital Tract Pathological Diagnosis: Our Experience and a Review on p16ink4a Functional Significance, Role in Daily-Practice Diagnosis, Prognostic Potential, and Technical Pitfalls. Diagnostics 2021, 11, 713. https://doi.org/10.3390/diagnostics11040713
Angelico G, Santoro A, Inzani F, Straccia P, Spadola S, Arciuolo D, Valente M, D’Alessandris N, Benvenuto R, Travaglino A, et al. An Emerging Anti-p16 Antibody-BC42 Clone as an Alternative to the Current E6H4 for Use in the Female Genital Tract Pathological Diagnosis: Our Experience and a Review on p16ink4a Functional Significance, Role in Daily-Practice Diagnosis, Prognostic Potential, and Technical Pitfalls. Diagnostics. 2021; 11(4):713. https://doi.org/10.3390/diagnostics11040713
Chicago/Turabian StyleAngelico, Giuseppe, Angela Santoro, Frediano Inzani, Patrizia Straccia, Saveria Spadola, Damiano Arciuolo, Michele Valente, Nicoletta D’Alessandris, Roberta Benvenuto, Antonio Travaglino, and et al. 2021. "An Emerging Anti-p16 Antibody-BC42 Clone as an Alternative to the Current E6H4 for Use in the Female Genital Tract Pathological Diagnosis: Our Experience and a Review on p16ink4a Functional Significance, Role in Daily-Practice Diagnosis, Prognostic Potential, and Technical Pitfalls" Diagnostics 11, no. 4: 713. https://doi.org/10.3390/diagnostics11040713
APA StyleAngelico, G., Santoro, A., Inzani, F., Straccia, P., Spadola, S., Arciuolo, D., Valente, M., D’Alessandris, N., Benvenuto, R., Travaglino, A., Raffone, A., & Zannoni, G. F. (2021). An Emerging Anti-p16 Antibody-BC42 Clone as an Alternative to the Current E6H4 for Use in the Female Genital Tract Pathological Diagnosis: Our Experience and a Review on p16ink4a Functional Significance, Role in Daily-Practice Diagnosis, Prognostic Potential, and Technical Pitfalls. Diagnostics, 11(4), 713. https://doi.org/10.3390/diagnostics11040713