Comparing Clinicopathological and Immunohistochemical Features of Colorectal Carcinoma between Young and Old Age Groups
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Design and Ethical Issues
2.2. Tissue Microarray
2.3. Immunohistochemical Analysis
2.4. Statistical Analysis
3. Results
3.1. Cases
3.2. Histopathological Features
3.3. Mismatch Repair Protein (MMR) Status
3.4. Immunohistochemical Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Stain | Antibody Used | Positive Control | Staining Pattern |
---|---|---|---|
MSH 6 | Clone 44 (Roche) (Basel, Switzerland) | Colon | Nuclear |
MLH 1 | Clone M1 (Roche) | Colon | Nuclear |
MSH 2 | Clone G219-1129 (Roche) | Colon | Nuclear |
PMS 2 | Clone EPR3947 (Roche) | Colon | Nuclear |
PD-L1 | Clone 22C.3 (Dako) (Glostrup, Denmark) | Placenta and tonsil | Membranous in tumor cells. Membranous or cytoplasmic in TILs |
BRAF | BRAF V600E (VE1) (Roche) | Melanoma | Cytoplasmic |
Ki67 | Clone 30-9 (Roche) | Lymphoma | Nuclear |
P53 | Clone DO7, (Roche) | Ovarian tumor | Nuclear |
Beta catenin | Clone 14 (Sigma-Aldrich) (Burlington, MA, USA) | Colonic cancer | Nuclear |
Young Group Total: 90 | Old Group Total: 90 | ||
---|---|---|---|
Characteristic | Number (%) | Number (%) | p Value |
Gender | 0.73 | ||
Female | 37 (41) | 35 (39) | |
Male | 53 (59) | 45 (61) | |
Site | 0.03 | ||
Rt colon | 55 (61) | 40 (44.4) | |
Lt colon | 35 (38.9) | 50 (55.6) | |
Histological type | 0.01 | ||
Adenocarcinoma | 70 (77.8) | 81 (90) | |
Mucinous | 20 (22.2) | 9 (10) | |
Grade | 0.00 | ||
Low grade | 60 (66.7) | 82 (91.1) | |
High grade | 30 (33.3) | 8 (8.9) | |
LVA | 0.76 | ||
Present | 31 | 33 | |
Absent | 59 | 57 | |
Perineural invasion | 0.28 | ||
Present | 75 (83.3) | 80 (88.9) | |
Absent | 15 (16.7) | 10 (11.1) | |
T stage | 0.11 | ||
T1/T2 | 11 (12.2) | 19 (21.1) | |
T3/T4 | 79 (87.8) | 71 (78.9) | |
N stage | 0.88 | ||
N0 | 41 | 40 (44.4) | |
N1/N2 | 49 | 50 (55.6) | |
M stage * | 0.24 | ||
M1 | 10 (33.3) | 17 (29.8) | |
M2 | 20 (66.7) | 40 (70.2) |
Young Group Total: 45 | Old Group Total: 45 | ||
---|---|---|---|
Stain | Number (%) | Number (%) | p Value |
dMMR | 20 (44.4) | 5 (11.1) | 0.00 |
pMMR | 25 (55.6) | 40 (88.9) | |
PD-L1 in tumor cells | 0.04 | ||
Positive | 14 (31.1) | 6 (13.3) | |
negative | 31 (68.9) | 39 (86.7) | |
PD-L1 in TILs | 0.67 | ||
Positive | 23 (51.1) | 21 (46.7) | |
Negative | 22 (48.9) | 24 (53.3) | |
BRAF | 1.00 | ||
Positive | 7 (15.6) | 7 (15.6) | |
Negative | 38 (84.4) | 38 (84.4) | |
Nuclear beta catenin | 0.15 | ||
Positive | 9 (20) | 15 (33.3) | |
Negative | 36 (80) | 30 (66.7) | |
P53 | 0.00 | ||
Lost or mutated | 18 (40) | 36 (80) | |
Wild type | 27 (60) | 9 (20) | |
Ki67 | 0.83 | ||
High >50% | 23 (51.1) | 22 (48.9) | |
Low | 22 (48.9) | 23 (51.1) |
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Awad, H.; Elshebli, S.; Hasan, K.; Eid, Y.; Obeidat, F.; Alzyoud, M.; Alakhras, B.; AlShammas, F. Comparing Clinicopathological and Immunohistochemical Features of Colorectal Carcinoma between Young and Old Age Groups. Diagnostics 2024, 14, 1743. https://doi.org/10.3390/diagnostics14161743
Awad H, Elshebli S, Hasan K, Eid Y, Obeidat F, Alzyoud M, Alakhras B, AlShammas F. Comparing Clinicopathological and Immunohistochemical Features of Colorectal Carcinoma between Young and Old Age Groups. Diagnostics. 2024; 14(16):1743. https://doi.org/10.3390/diagnostics14161743
Chicago/Turabian StyleAwad, Heyam, Sanad Elshebli, Khalid Hasan, Yousef Eid, Fatima Obeidat, Mohammad Alzyoud, Basheer Alakhras, and Faris AlShammas. 2024. "Comparing Clinicopathological and Immunohistochemical Features of Colorectal Carcinoma between Young and Old Age Groups" Diagnostics 14, no. 16: 1743. https://doi.org/10.3390/diagnostics14161743