Effect of Insulin Receptor on Juvenile Hormone Signal and Fecundity in Spodoptera litura (F.)
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Insect Rearing
2.2. Bovine Insulin Microinjection
2.3. RNA Isolation and Quantitative Real-Time-Polymerase Chain Reaction (qRT-PCR)
2.4. Sample Collection for Different Tissue Expression Analysis
2.5. RNA Interference
2.6. Determination of Protein and Triglyceride (TAG)
2.7. JH- III Determination
2.8. Western Blotting
2.9. Ovary Dissection and Microscopy
2.10. Statistical Analysis
3. Results
3.1. Tissue-Specific Expression of SlInR
3.2. Changes in Insulin Pathway Gene Expression after Bovine Insulin Application
3.3. Effect of SlInR Silence on Insulin Pathway Gene expression
3.4. Effect of SlInR Knockdown on Protein and Triglyceride (TAG) Metabolism
3.5. Effect of SlInR Silence on SlVg Expression and Reproduction
3.6. Effect of SlInR Interference on SlMet Expression and JH-III titer
3.7. Effect of SlInR Knockdown on Ovarian Development
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Name | Sequence (5′-3′) |
---|---|---|
For qRT-PCR | ||
SlInR (XM_022966387.1) | SlInR- qF | CATTGACGCCGTCTGGTTTG |
SlInR- qR | CAGCGGAGGTGTTTCCTGAG | |
SlPI3K (XM_022968595.1) | SlPI3K- qF | ATACATGACGAGTACGCCCGAG |
SlPI3K- qR | TCAGTACGGAGGCAGACAGTAG | |
SlAKT (XM_022971351.1) | SlAKT- qF | GGACAAGGACGGACACATCAAG |
SlAKT- qR | GCTCAGGATCAGCGAGAACAAC | |
SlFoxO (XM_022969964.1) | SlFoxO- qF | AACGGACTTCGGACAAGACG |
SlFoxO- qR | TCGCGGACACCCACTATAAG | |
SlTOR (XM_022966802.1) | SlTOR- qF | GACAGAACAACAGCCAAGGGAG |
SlTOR- qR | CGGAAGTGGAGTCAGAAACAGG | |
SlS6K (XM_022972696.1) | SlS6K- qF | AGGACAGACCCAGGATGATGAC |
SlS6K- qR | ATAGACGACCCCGAGACTCCAC | |
SlCREB (XM_022973491.1) | SlCREB- qF | CTTCAATGACATCCAGGGCGAC |
SlCREB- qR | ATTTGATGCTGCTTCCTCCCAC | |
SlVg (EU095334.1) | SlVg- qF | CCCACCACACTCTTGCTTTCTC |
SlVg- qR | GTCCTTGTTCACGTTCCCTGTC | |
SlMet (2595041) | SlMet- qF | ACGCTCTGACAAAGCAAC |
SlMet- qR | CTGGCATCGAACTAGACAATAC | |
SlRPL10 (KC866373.1) | SlRPL10- qF | GACTTGGGTAAGAAGAAG |
SlRPL10- qR | GATGACATGGAATGGATG | |
For SlInR dsRNA synthesis | ||
SlInR (XM_022966387.1) | SlInR ds- F1 | TCGGAATGGTATACGAAGGC |
SlInR ds- R1 | TCTGGTCATACCGAAGTCCC | |
T7 SlInR ds- F | GGATCCTAATACGACTCACTATAGGG-TCGGAATGGTATACGAAGGC | |
T7 SlInR ds- R | GGATCCTAATACGACTCACTATAGGG-TCTGGTCATACCGAAGTCCC | |
EGFP DQ768212 | EGFP ds- F1 | GCTGACCCTGAAGTTCATCTG |
EGFP ds- R1 | GAACTCCAGCAGGACCATGT | |
T7 EGFP ds- F | GGATCCTAATACGACTCACTATAGGG-GCTGACCCTGAAGTTCATCTG | |
T7 EGFP ds- R | GGATCCTAATACGACTCACTATAGGG-GAACTCCAGCAGGACCATGT |
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Share and Cite
Pan, X.; Pei, Y.; Zhang, C.; Huang, Y.; Chen, L.; Wei, L.; Li, C.; Dong, X.; Chen, X. Effect of Insulin Receptor on Juvenile Hormone Signal and Fecundity in Spodoptera litura (F.). Insects 2022, 13, 701. https://doi.org/10.3390/insects13080701
Pan X, Pei Y, Zhang C, Huang Y, Chen L, Wei L, Li C, Dong X, Chen X. Effect of Insulin Receptor on Juvenile Hormone Signal and Fecundity in Spodoptera litura (F.). Insects. 2022; 13(8):701. https://doi.org/10.3390/insects13080701
Chicago/Turabian StylePan, Xue, Yanfang Pei, Cuici Zhang, Yaling Huang, Ling Chen, Liqiong Wei, Chuanren Li, Xiaolin Dong, and Xiang Chen. 2022. "Effect of Insulin Receptor on Juvenile Hormone Signal and Fecundity in Spodoptera litura (F.)" Insects 13, no. 8: 701. https://doi.org/10.3390/insects13080701