The Genus Heterogynis Rambur, 1866 (Heterogynidae, Lepidoptera): Congruence of Molecular, Morphological and Morphometric Evidence Reveal New Species in Serbia
Abstract
:Simple Summary
Abstract
1. Introduction
2. Material and Methods
2.1. Collection and Morphological Analysis
2.2. Morphometric Analysis
2.3. DNA Isolation, Amplification and Sequencing
Primers for COI Genes | Primer Sequences | Species | Reference |
---|---|---|---|
LepF1 | 5′-ATTCAACCAATCATAAAGATATTG-3′ | H. affinis | [3] |
LepR1 | 5′-TAAACTTCTGGATGTCCAAAAAATC-3′ | H. cynetis | |
LEP-F1 | 5′-ATTCAACCAATCATAAAGATAT-3′; | Astraptes fulgerator | [25] |
LEP-R1 | 5′-TAAACTTCTGGATGTCCAAAAA-3′ | ||
LEP-R2 | 5′-CTTATATTATTTATTCGTGGGAAAGC-3′ | ||
LCO1490 | 5′-GGTCAACAAATCATAAAGATATTGG-3′ | Diverse metazoan invertebrate | [29] |
HCO2198 | 5′-TAAACTTCAGGGTGACCAAAAAATCA-3′ | ||
LCO1490 | 5′-GGTCAACAAATCATAAAGATATTGG-3′ | Taxon barcode | [14,29] |
HCO2198 | 5′-TAAACTTCAGGGTGACCAAAAAATCA-3′ | ||
CO1-exF | 5′-ATCGCCTAAACTTCAGCCATT-3′ | Cucullia umbratica | [30,31] |
TL-N-3017 | 5′-CTTAAATCCATTGCACTAATCTGCCATA-3′ | ||
“Jerry” | 5′-CAACATTTATTTTGATTTTTTGG-3′ | Crematogaster fraxatrix | [27,28] |
“Pat” | 5′-TCCAATGCACTAATCTGCCATATTA- 3′ |
2.4. Nucleotide Sequence Analyses
3. Results
3.1. Taxonomy
3.2. Morphological Diagnosis
3.3. Morphometric Analysis
3.4. Molecular Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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PCs | Eigenvalue | % Total (Variance) | Cumulative (%) |
---|---|---|---|
PC1 | 4.6324 | 77.21 | 77.21 |
PC2 | 0.87283 | 14.55 | 91.75 |
PC3 | 0.36108 | 6.02 | 97.77 |
PC4 | 0.11374 | 1.90 | 99.67 |
PC5 | 0.01984 | 0.33 | 99.99 |
PC6 | 0.00012 | 0.00 | 100.00 |
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Stojanović, D.V.; Galović, V.; Terzin, T.; Ačanski, J.; Vidović, M.; Orlović, S. The Genus Heterogynis Rambur, 1866 (Heterogynidae, Lepidoptera): Congruence of Molecular, Morphological and Morphometric Evidence Reveal New Species in Serbia. Insects 2023, 14, 455. https://doi.org/10.3390/insects14050455
Stojanović DV, Galović V, Terzin T, Ačanski J, Vidović M, Orlović S. The Genus Heterogynis Rambur, 1866 (Heterogynidae, Lepidoptera): Congruence of Molecular, Morphological and Morphometric Evidence Reveal New Species in Serbia. Insects. 2023; 14(5):455. https://doi.org/10.3390/insects14050455
Chicago/Turabian StyleStojanović, Dejan V., Vladislava Galović, Tomislav Terzin, Jelena Ačanski, Marija Vidović, and Saša Orlović. 2023. "The Genus Heterogynis Rambur, 1866 (Heterogynidae, Lepidoptera): Congruence of Molecular, Morphological and Morphometric Evidence Reveal New Species in Serbia" Insects 14, no. 5: 455. https://doi.org/10.3390/insects14050455