Management of Spodoptera frugiperda J.E. Smith Using Recycled Virus Inoculum from Larvae Treated with Baculovirus under Field Conditions
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Study Site and Experimental Plots
2.2. Maize Seedlings
2.3. Fall Armyworm Colony
2.4. Laboratory Evaluation of Efficacy of Recycled Virus Inoculum from FAW Larvae Sprayed with Littovir
2.4.1. Inoculum Preparation
- (a)
- Initial inoculum treatment with baculovirus (Littovir)
- (b)
- Virus from FAW larvae treated with baculovirus (Littovir)
2.4.2. Treatment
2.5. Field Evaluation of Efficacy of Recycled Virus Inoculum from FAW Larvae Sprayed with a Commercial Baculovirus Product
2.5.1. Initial Inoculum for Field Evaluation and Seed Bed Preparation
2.5.2. Engeo 247SC (Sygenta) and Escort 1.9EC (Green Life Crop Protection)
2.5.3. Soil and Weather Conditions in Muguga
2.5.4. Crop
2.5.5. Treatments, Layout and Design
2.6. Evaluation of Treatments
2.6.1. FAW Infestation Assessment
2.6.2. FAW Leaf Damage Assessment
2.6.3. Maize Grain Yield Assessment
2.7. Statistical Analysis
3. Results
3.1. Laboratory Experiment
3.1.1. Effect of Baculovirus (Littovir) on Different FAW Developmental Stages
3.1.2. Field Experiments
Effects of Treatments on FAW Damage to Maize Crop
Maize Grain Yield
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatment | FAW Instar | Mean Mortality + SE |
---|---|---|
Inoculum recovered from 25 larvae | 1st–3rd | 8.3 ± 1 d |
Inoculum recovered from 50 larvae | 1st–3rd | 16.5 ± 2 dc |
Inoculum recovered from 75 larvae | 1st–3rd | 22.5 ± 4 c |
Inoculum recovered from 100 larvae | 1st–3rd | 36.2 ± 5 b |
Littovir | 1st–3rd | 44.8 ± 5 a |
Treatment | FAW Instar | Mean Mortality + SE |
---|---|---|
Inoculum recovered from 25 larvae | 4th–6th | 5.2 ± 0.2 c |
Inoculum recovered from 50 larvae | 4th–6th | 9.6 ± 0.8 bc |
Inoculum recovered from 75 larvae | 4th–6th | 12.1 ± 1.4 bc |
Inoculum recovered from 100 larvae | 4th–6th | 15.4 ± 1.6 ab |
Littovir | 4th–6th | 21.7 ± 2.1 a |
Treatment | Mean Crop Damage Score + SE |
---|---|
Escort | 1.72 ± 0.2 a |
Engeo | 2.00 ± 0.1 ab |
Littovir | 2.73 ± 0.4 bc |
Inoculum recovered from 100 FAW larvae | 2.80 ± 0.2 c |
Inoculum recovered from 150 FAW larvae | 2.80 ± 0.3 ab |
Inoculum recovered from 200 FAW larvae | 2.84 ± 0.3 c |
Inoculum recovered from 250 FAW larvae | 2.87 ± 0.4 c |
Untreated control | 3.30 ± 0.4 c |
Treatment | Mean Grain Yield + SE (tons/ha) |
---|---|
Escort | 4.4 ± 0.4 a |
Engeo | 4.0 ± 0.3 ab |
Littovir | 3.1 ± 0.5 ab |
Inoculum recovered from 100 FAW larvae | 3.0 ± 0.4 ab |
Inoculum recovered from 150 FAW larvae | 3.0 ± 0.4 ab |
Inoculum recovered from 200 FAW larvae | 3.0 ± 0.4 ab |
Inoculum recovered from 250 FAW larvae | 3.04 ± 0.4 ab |
Untreated control | 2.4 ± 0.4 b |
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Mweke, A.; Rwomushana, I.; Okello, A.; Chacha, D.; Guo, J.; Luke, B. Management of Spodoptera frugiperda J.E. Smith Using Recycled Virus Inoculum from Larvae Treated with Baculovirus under Field Conditions. Insects 2023, 14, 686. https://doi.org/10.3390/insects14080686
Mweke A, Rwomushana I, Okello A, Chacha D, Guo J, Luke B. Management of Spodoptera frugiperda J.E. Smith Using Recycled Virus Inoculum from Larvae Treated with Baculovirus under Field Conditions. Insects. 2023; 14(8):686. https://doi.org/10.3390/insects14080686
Chicago/Turabian StyleMweke, Allan, Ivan Rwomushana, Arthur Okello, Duncan Chacha, Jingfei Guo, and Belinda Luke. 2023. "Management of Spodoptera frugiperda J.E. Smith Using Recycled Virus Inoculum from Larvae Treated with Baculovirus under Field Conditions" Insects 14, no. 8: 686. https://doi.org/10.3390/insects14080686