Combined Analysis of Metabolomics and Biochemical Changes Reveals the Nutritional and Functional Characteristics of Red Palm Weevil Rhynchophus ferrugineus (Coleoptera: Curculionidae) Larvae at Different Developmental Stages
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Samples Preparation
2.2. Determination of Chemical Composition
2.3. Determination of Amino Acid Composition
2.4. Determination of Fatty Acid Composition
2.5. Determination of Mineral Composition
2.6. Determination of Untargeted Metabolomics
2.6.1. Metabolite Extraction
2.6.2. LC-MS/MS Analysis
2.6.3. Data Preprocessing
2.7. Antioxidant Activities In Vitro of RPW Larvae Extract
2.7.1. Preparation of RPW Larvae Extract
2.7.2. DPPH Radical-Scavenging Activity Assay
2.7.3. ABTS Radical-Scavenging Activity Assay
2.7.4. Hydroxyl Radical-Scavenging Activity Assay
2.8. Statistical Analysis
3. Results and Analysis
3.1. Analysis of Chemical Composition
3.2. Amino Acid Composition Analysis
3.3. Analysis of Fatty Acid Composition
3.4. Minerals Composition Analysis
3.5. Metabonomic Analysis
3.5.1. Characterization of Metabolites
3.5.2. Multivariate Analysis of Differential Metabolites
3.5.3. KEGG Annotation and Enrichment Analysis of Differential Metabolites
3.6. Analysis of Antioxidant Activities In Vitro
4. Conclusions and Discussion
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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EL | ML | OL | |
---|---|---|---|
Anthropometric property | |||
Length (mm) | 1.55 ± 0.36 c | 3.23 ± 0.35 b | 4.99 ± 0.28 a |
Weight (g) | 0.93 ± 0.22 c | 4.60 ± 0.88 b | 6.30 ± 0.58 a |
Diameter (cm) | 0.53 ± 0.13 c | 1.33 ± 0.14 b | 1.56 ± 0.14 a |
Head circumference (cm) | 0.17 ± 0.05 c | 0.43 ± 0.06 b | 0.63 ± 0.02 a |
Chemical composition | |||
Moisture (g/100 g, fw) | 80.70 ± 0.34 a | 59.95 ± 0.05 b | 57.16 ± +0.67 c |
Ash (g/100 g, dw) | 3.59 ± 0.44 a | 2.22 ± 0.09 b | 2.17 ± 0.15 b |
Protein (g/100 g, dw) | 53.87 ± 0.20 a | 34.23 ± 0.17 b | 32.10 ± 0.21 c |
Fat (g/100 g, dw) | 18.55 ± 0.18 c | 67.95 ± 0.39 a | 58.45 ± 0.84 b |
Total phenolic content (mg GAE/g, dw) | 21.10 ± 0.06 a | 6.07 ± 0.16 b | 4.47 ± 0.04 c |
EL | ML | OL | |
---|---|---|---|
EAA | 25.27 ± 1.16 b | 26.28 ± 1.17 a | 19.46 ± 0.95 c |
His | 1.72 ± 0.08 a | 1.72 ± 0.15 a | 1.29 ± 0.09 b |
Met | 0.68 ± 0.16 c | 1.00 ± 0.03 a | 0.81 ± 0.04 b |
Val | 3.76 ± 0.15 b | 4.08 ± 0.10 a | 3.05 ± 0.24 c |
Ile | 2.72 ± 0.20 b | 3.19 ± 0.22 a | 2.44 ± 0.11 c |
Leu | 4.89 ± 0.13 a | 4.88 ± 0.08 b | 3.55 ± 0.30 c |
Phe | 2.77 ± 0.13 b | 2.83 ± 0.22 a | 2.28 ± 0.09 c |
Thr | 2.88 ± 0.15 b | 3.04 ± 0.06 a | 2.29 ± 0.02 c |
Lys | 5.84 ± 0.17 a | 5.54 ± 0.57 b | 3.76 ± 0.18 c |
NEAA | 36.28 ± 0.69 b | 40.26 ± 1.33 a | 29.18 ± 0.65 c |
Cys | 0.16 ± 0.05 c | 0.46 ± 0.03 b | 0.53 ± 0.12 a |
Tyr | 2.85 ± 0.45 b | 3.14 ± 0.36 a | 2.14 ± 0.30 c |
Asp | 5.04 ± 0.70 b | 6.53 ± 0.17 a | 4.82 ± 0.21 c |
Ser | 2.84 ± 0.16 b | 3.18 ± 0.11 a | 2.71 ± 0.13 c |
Glu | 9.88 ± 0.11 a | 9.75 ± 0.37 b | 6.86 ± 0.11 c |
Gly | 3.06 ± 0.3 c | 3.62 ± 0.19 a | 3.07 ± 0.21 b |
Ala | 4.39 ± 0.06 b | 4.42 ± 0.28 a | 3.26 ± 0.11 c |
Arg | 4.19 ± 0.23 a | 4.14 ± 0.05 b | 3.10 ± 0.20 c |
Pro | 3.86 ± 0.12 b | 5.01 ± 0.40 a | 2.68 ± 0.34 c |
TAA | 61.55 ± 1.56 b | 66.54 ± 2.44 a | 48.64 ± 1.60 c |
EAA/TAA (%) | 41.05 ± 0.94 a | 39.49 ± 0.46 c | 40.00 ± 0.65 b |
EAA/NEAA (%) | 69.67 ± 2.73 a | 65.27 ± 1.25 c | 66.69 ± 1.79 b |
Fatty Acids | EL | ML | OL |
---|---|---|---|
Caprylic acid (C8:0) | 0.11 ± 0.00 b | 0.15 ± 0.02 b | 0.37 ± 0.12 a |
Capric acid (C10:0) | 0.10 ± 0.00 b | 0.13 ± 0.02 b | 0.32 ± 0.09 a |
Lauric acid (C12:0) | 0.86 ± 0.01 b | 1.09 ± 0.18 b | 2.65 ± 0.76 a |
Myristic acid (C14:0) | 1.65 ± 0.01 b | 1.69 ± 0.06 b | 2.21 ± 0.28 a |
Palmitic acid (C16:0) | 43.01 ± 0.07 a | 41.41 ± 0.63 b | 39.57 ± 0.41 c |
Palmitoleic acid (C16:1) | 6.01 ± 0.03 a | 5.53 ± 0.11 c | 5.82 ± 0.09 b |
Stearic acid (C18:0) | 1.80 ± 0.03 b | 2.10 ± 0.01 a | 1.68 ± 0.05 c |
Oleic acid (C18:1) | 45.56 ± 0.10 b | 46.79 ± 0.39 a | 45.22 ± 0.81 b |
Linoleic acid (C18:2 n6) | 0.51 ± 0.03 b | 0.54 ± 0.01 b | 1.31 ± 0.02 a |
α-Linolenic acid (C18:3 n3) | 0.11 ± 0.01 c | 0.17 ± 0.00 b | 0.24 ± 0.00 a |
Behenic acid (C22:0) | 0.30 ± 0.00 a | 0.31 ± 0.00 a | 0.2 ± 0.03 b |
Nervonic acid (C24:1) | ND | 0.1 ± 0.00 b | 0.42 ± 0.02 a |
SFA | 47.83 ± 0.01 a | 46.88 ± 0.00 b | 46.99 ± 0.14 b |
UFA | 52.17 ± 0.03 c | 53.12 ± 0.01 a | 53.01 ± 0.63 b |
MUFA | 51.56 ± 0.76 b | 52.42 ± 0.01 a | 51.46 ± 0.81 c |
PUFA | 0.62 ± 0.00 c | 0.71 ± 0.00 b | 1.55 ± 0.00 a |
PUFA/SFA | 0.01 ± 0.00 b | 0.01 ± 0.00 b | 0.03 ± 0.00 a |
n-6/n-3 | 4.67 ± 0.25 b | 3.19 ± 0.05 c | 5.50 ± 0.05 a |
Mineral | EL | ML | OL |
---|---|---|---|
Macrominerals | |||
Potassium | 15,865.04 ± 76.76 a | 10,568.25 ± 117.74 b | 5707.12 ± 33.35 c |
Phosphorus | 7728.37 ± 91.87 a | 4166.53 ± 41.22 b | 2123.87 ± 12.51 c |
Magnesium | 2030.59 ± 15.54 a | 1942.75 ± 46.37 b | 1123.21 ± 1.32 c |
Sodium | 1907.21 ± 9.7 a | 1798.43 ± 41.64 b | 707.79 ± 3.25 c |
Calcium | 700.73 ± 22.6 a | 594.84 ± 16.74 b | 341.86 ± 4.97 c |
Microminerals | |||
Zinc | 211.00 ± 4.87 a | 112.29 ± 4.56 b | 49.64 ± 1.11 c |
Iron | 79.35 ± 3.23 a | 29.48 ± 0.86 b | 12.25 ± 0.67 c |
Copper | 13.91 ± 0.3 a | 5.84 ± 0.01 b | 5.87 ± 0.04 b |
Manganese | 10.47 ± 0.22 a | 7.16 ± 0.26 b | 4.34 ± 0.03 c |
Chromium | 1.44 ± 0.09 a | 0.28 ± 0.02 b | 0.16 ± 0.03 c |
Nickel | 1.18 ± 0.01 a | 0.67 ± 0.04 b | 0.08 ± 0.01 c |
Arsenic | 0.07 ± 0.00 a | 0.05 ± 0.00 a | 0.01 ± 0.00 b |
Cadmium | 0.33 ± 0.01 a | 0.09 ± 0.00 b | 0.02 ± 0.00 c |
Lead | 0.16 ± 0.01 a | 0.14 ± 0.00 a | 0.01 ± 0.00 b |
Name | Number | Proportion% |
---|---|---|
Lipids and lipid-like molecules | 87 | 27.02 |
Organic acids and derivatives | 79 | 24.53 |
Organoheterocyclic compounds | 48 | 14.91 |
Organic oxygen compounds | 33 | 10.25 |
Benzenoids | 30 | 9.32 |
Organic nitrogen compounds | 15 | 4.66 |
Phenylpropanoids and polyketides | 14 | 4.35 |
Nucleosides, nucleotides, and analogues | 13 | 4.04 |
Alkaloids and derivatives | 2 | 0.62 |
Homogeneous non-metal compounds | 1 | 0.31 |
Total | 322 | 100 |
Radicals | Samples | EL | ML | OL | |
---|---|---|---|---|---|
EC50 value | DPPH | Water extract | 1.12 ± 0.01 c | 3.94 ± 0.02 a | 1.88 ± 0.01 b |
Ethanol extract | 15.35 ± 0.03 c | 25.83 ± 0.17 b | 27.69 ± 0.01 a | ||
Hydroxyl | Water extract | 11.23 ± 0.01 c | 16.02 ± 0.06 b | 17.73 ± 0.04 a | |
Ethanol extract | 58.12 ± 0.03 a | 36.47 ± 0.08 c | 39.43 ± 0.12 b | ||
ABTS | Water extract | 2.52 ± 0.17 c | 6.22 ± 0.70 a | 4.86 ± 0.35 b | |
Ethanol extract | 17.42 ± 0.29 c | 49.63 ± 0.81 a | 45.13 ± 0.26 b |
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Chen, M.; Kan, J.; Zhang, Y.; Zhao, J.; Lv, C.; Zhong, B.; Li, C.; Qin, W. Combined Analysis of Metabolomics and Biochemical Changes Reveals the Nutritional and Functional Characteristics of Red Palm Weevil Rhynchophus ferrugineus (Coleoptera: Curculionidae) Larvae at Different Developmental Stages. Insects 2024, 15, 294. https://doi.org/10.3390/insects15040294
Chen M, Kan J, Zhang Y, Zhao J, Lv C, Zhong B, Li C, Qin W. Combined Analysis of Metabolomics and Biochemical Changes Reveals the Nutritional and Functional Characteristics of Red Palm Weevil Rhynchophus ferrugineus (Coleoptera: Curculionidae) Larvae at Different Developmental Stages. Insects. 2024; 15(4):294. https://doi.org/10.3390/insects15040294
Chicago/Turabian StyleChen, Mengran, Jintao Kan, Yufeng Zhang, Jinhao Zhao, Chaojun Lv, Baozhu Zhong, Chaoxu Li, and Weiquan Qin. 2024. "Combined Analysis of Metabolomics and Biochemical Changes Reveals the Nutritional and Functional Characteristics of Red Palm Weevil Rhynchophus ferrugineus (Coleoptera: Curculionidae) Larvae at Different Developmental Stages" Insects 15, no. 4: 294. https://doi.org/10.3390/insects15040294