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Open AccessArticle
Molecular Identification and Bioinformatics Analysis of Anaplasma marginale Moonlighting Proteins as Possible Antigenic Targets
by
Rosa Estela Quiroz-Castañeda
Rosa Estela Quiroz-Castañeda *,†,
Hugo Aguilar-Díaz
Hugo Aguilar-Díaz †,
Eduardo Coronado-Villanueva
Eduardo Coronado-Villanueva ,
Diego Israel Catalán-Ochoa
Diego Israel Catalán-Ochoa and
Itzel Amaro-Estrada
Itzel Amaro-Estrada
Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (CENID-SAI, INIFAP), Jiutepec 62574, México
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Pathogens 2024, 13(10), 845; https://doi.org/10.3390/pathogens13100845 (registering DOI)
Submission received: 7 August 2024
/
Revised: 13 September 2024
/
Accepted: 27 September 2024
/
Published: 28 September 2024
Abstract
Background: Diseases of veterinary importance, such as bovine Anaplasmosis, cause significant economic losses. Due to this, the study of various proteins of the causal agent Anaplasma marginale has focused on surface proteins. However, a vaccine for this disease is not yet available. To this end, in this work, moonlighting proteins (MLPs) are presented as an alternative approach for the design of immunogens against A. marginale. Methods: The proteins of the strain MEX-15-099-01 were analyzed, and its MLPs were identified. Subsequently, four virulence-associated MLP genes were selected and identified using PCR. The proteins were analyzed using a structural homology approach and the collection of B-cell epitopes was predicted for each MLP. Finally, a pair of AmEno peptides were synthesized and the antigenic potential was tested using an iELISA. Results: Our bioinformatics analysis revealed the potential of AmEno, AmGroEl, AmEF-Tu, and AmDnaK proteins as promising candidates for designing immunogens. The PCR allowed the gene sequence identification in the genome of the strain MEX-15-099-01. Notably, AmEno-derived synthetic peptides showed antigenicity in an ELISA. Conclusions: Our study has shed light on the potential use of MLPs for immunogen design, demonstrating the antigenic potential of AmEno.
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MDPI and ACS Style
Quiroz-Castañeda, R.E.; Aguilar-Díaz, H.; Coronado-Villanueva, E.; Catalán-Ochoa, D.I.; Amaro-Estrada, I.
Molecular Identification and Bioinformatics Analysis of Anaplasma marginale Moonlighting Proteins as Possible Antigenic Targets. Pathogens 2024, 13, 845.
https://doi.org/10.3390/pathogens13100845
AMA Style
Quiroz-Castañeda RE, Aguilar-Díaz H, Coronado-Villanueva E, Catalán-Ochoa DI, Amaro-Estrada I.
Molecular Identification and Bioinformatics Analysis of Anaplasma marginale Moonlighting Proteins as Possible Antigenic Targets. Pathogens. 2024; 13(10):845.
https://doi.org/10.3390/pathogens13100845
Chicago/Turabian Style
Quiroz-Castañeda, Rosa Estela, Hugo Aguilar-Díaz, Eduardo Coronado-Villanueva, Diego Israel Catalán-Ochoa, and Itzel Amaro-Estrada.
2024. "Molecular Identification and Bioinformatics Analysis of Anaplasma marginale Moonlighting Proteins as Possible Antigenic Targets" Pathogens 13, no. 10: 845.
https://doi.org/10.3390/pathogens13100845
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