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Microorganisms
Volume 10
Issue 4
10.3390/microorganisms10040762
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Article

The Effect of Amino Acids on Production of SCFA and bCFA by Members of the Porcine Colonic Microbiota

by
Pieter Van den Abbeele
1,2,
Jonas Ghyselinck
1,
Massimo Marzorati
1,3,
Anna-Maria Koch
4,
William Lambert
4,
Joris Michiels
5 and
Tristan Chalvon-Demersay
4,*
1
ProDigest, 9052 Ghent, Belgium
2
Cryptobiotix, 9052 Ghent, Belgium
3
Center of Microbial Ecology and Technology (CMET), Ghent University, 9000 Ghent, Belgium
4
METEX NOOVISTAGO, 75017 Paris, France
5
Laboratory for Animal Nutrition and Animal Product Quality, Ghent University, 9000 Ghent, Belgium
*
Author to whom correspondence should be addressed.
Microorganisms 2022, 10(4), 762; https://doi.org/10.3390/microorganisms10040762
Submission received: 24 January 2022 / Revised: 14 March 2022 / Accepted: 28 March 2022 / Published: 31 March 2022
(This article belongs to the Special Issue Gut Microbiota and Nutrients)
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Abstract

Functional amino acids supplementation to farm animals is considered to not only be beneficial by regulating intestinal barrier, oxidative stress, and immunity, but potentially also by impacting the gut microbiota. The impact of amino acids on a piglet-derived colonic microbiota was evaluated using a 48-h in vitro batch incubation strategy. The combination of 16S rRNA gene profiling with flow cytometry demonstrated that specific microbial taxa were involved in the fermentation of each of the amino acids resulting in the production of specific metabolites. Branched chain amino acids (leucine, isoleucine, valine) strongly increased branched-chain fatty acids (+23.0 mM) and valerate levels (+3.0 mM), coincided with a marked increase of Peptostreptococcaceae. Further, glutamine and glutamate specifically stimulated acetate (~20 mM) and butyrate (~10 mM) production, relating to a stimulation of a range of families containing known butyrate-producing species (Ruminococcaceae, Oscillospiraceae, and Christensenellaceae). Finally, while tryptophan was only fermented to a minor extent, arginine and lysine specifically increased propionate levels (~2 mM), likely produced by Muribaculaceae members. Overall, amino acids were thus shown to be selectively utilized by microbes originating from the porcine colonic microbiota, resulting in the production of health-related short-chain fatty acids, thus confirming the prebiotic potential of specific functional amino acids.
Keywords: amino acids; gut microbiota; bCFA; SCFA; piglet; colon amino acids; gut microbiota; bCFA; SCFA; piglet; colon

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MDPI and ACS Style

Van den Abbeele, P.; Ghyselinck, J.; Marzorati, M.; Koch, A.-M.; Lambert, W.; Michiels, J.; Chalvon-Demersay, T. The Effect of Amino Acids on Production of SCFA and bCFA by Members of the Porcine Colonic Microbiota. Microorganisms 2022, 10, 762. https://doi.org/10.3390/microorganisms10040762

AMA Style

Van den Abbeele P, Ghyselinck J, Marzorati M, Koch A-M, Lambert W, Michiels J, Chalvon-Demersay T. The Effect of Amino Acids on Production of SCFA and bCFA by Members of the Porcine Colonic Microbiota. Microorganisms. 2022; 10(4):762. https://doi.org/10.3390/microorganisms10040762

Chicago/Turabian Style

Van den Abbeele, Pieter, Jonas Ghyselinck, Massimo Marzorati, Anna-Maria Koch, William Lambert, Joris Michiels, and Tristan Chalvon-Demersay. 2022. "The Effect of Amino Acids on Production of SCFA and bCFA by Members of the Porcine Colonic Microbiota" Microorganisms 10, no. 4: 762. https://doi.org/10.3390/microorganisms10040762

APA Style

Van den Abbeele, P., Ghyselinck, J., Marzorati, M., Koch, A.-M., Lambert, W., Michiels, J., & Chalvon-Demersay, T. (2022). The Effect of Amino Acids on Production of SCFA and bCFA by Members of the Porcine Colonic Microbiota. Microorganisms, 10(4), 762. https://doi.org/10.3390/microorganisms10040762

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