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Microorganisms
Volume 10
Issue 5
10.3390/microorganisms10051046
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Article

Sodium Citrate Alleviates Virulence in Pseudomonas aeruginosa

by
Maan T. Khayat
1,*,
Tarek S. Ibrahim
1,
Ahdab N. Khayyat
1,
Majed Alharbi
1,
Moataz A. Shaldam
2,
Khadijah A. Mohammad
1,
El-Sayed Khafagy
3,4,
Dalia A. El-damasy
5,
Wael A. H. Hegazy
6,7,* and
Hisham A. Abbas
6
1
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia
2
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Kafrelsheikh University, Kafr El-Sheikh 33511, Egypt
3
Department of Pharmaceutics, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-kharj 11942, Saudi Arabia
4
Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Suez Canal University, Ismailia 41552, Egypt
5
Department of Microbiology and Immunology, Faculty of Pharmacy, Egyptian Russian University, Tenth of Ramadan 44629, Egypt
6
Department of Microbiology and Immunology, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt
7
Pharmacy Program, Department of Pharmaceutical Sciences, Oman College of Health Sciences, Muscat 113, Oman
*
Authors to whom correspondence should be addressed.
Microorganisms 2022, 10(5), 1046; https://doi.org/10.3390/microorganisms10051046
Submission received: 18 April 2022 / Revised: 12 May 2022 / Accepted: 16 May 2022 / Published: 18 May 2022
(This article belongs to the Section Antimicrobial Agents and Resistance)
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Abstract

The development of bacterial resistance is an insistent global health care issue, especially in light of the dwindled supply of new antimicrobial agents. This mandates the development of new innovative approaches to overcome the resistance development obstacle. Mitigation of bacterial virulence is an interesting approach that offers multiple advantages. Employing safe chemicals or drugs to mitigate bacterial virulence is an additive advantage. In the current study, the in vitro antivirulence activities of citrate were evaluated. Significantly, sodium citrate inhibited bacterial biofilm formation at sub-MIC concentrations. Furthermore, sodium citrate decreased the production of virulence factors protease and pyocyanin and diminished bacterial motility. Quorum sensing (QS) is the communicative system that bacterial cells utilize to communicate with each other and regulate the virulence of the host cells. In the present study, citrate in silico blocked the Pseudomonas QS receptors and downregulated the expression of QS-encoding genes. In conclusion, sodium citrate showed a significant ability to diminish bacterial virulence in vitro and interfered with QS; it could serve as a safe adjuvant to traditional antibiotic treatment for aggressive resistant bacterial infections such as Pseudomonas aeruginosa infections.
Keywords: Pseudomonas aeruginosa; sodium citrate; bacterial virulence; quorum sensing; bacterial resistance; industrial developments Pseudomonas aeruginosa; sodium citrate; bacterial virulence; quorum sensing; bacterial resistance; industrial developments

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MDPI and ACS Style

Khayat, M.T.; Ibrahim, T.S.; Khayyat, A.N.; Alharbi, M.; Shaldam, M.A.; Mohammad, K.A.; Khafagy, E.-S.; El-damasy, D.A.; Hegazy, W.A.H.; Abbas, H.A. Sodium Citrate Alleviates Virulence in Pseudomonas aeruginosa. Microorganisms 2022, 10, 1046. https://doi.org/10.3390/microorganisms10051046

AMA Style

Khayat MT, Ibrahim TS, Khayyat AN, Alharbi M, Shaldam MA, Mohammad KA, Khafagy E-S, El-damasy DA, Hegazy WAH, Abbas HA. Sodium Citrate Alleviates Virulence in Pseudomonas aeruginosa. Microorganisms. 2022; 10(5):1046. https://doi.org/10.3390/microorganisms10051046

Chicago/Turabian Style

Khayat, Maan T., Tarek S. Ibrahim, Ahdab N. Khayyat, Majed Alharbi, Moataz A. Shaldam, Khadijah A. Mohammad, El-Sayed Khafagy, Dalia A. El-damasy, Wael A. H. Hegazy, and Hisham A. Abbas. 2022. "Sodium Citrate Alleviates Virulence in Pseudomonas aeruginosa" Microorganisms 10, no. 5: 1046. https://doi.org/10.3390/microorganisms10051046

APA Style

Khayat, M. T., Ibrahim, T. S., Khayyat, A. N., Alharbi, M., Shaldam, M. A., Mohammad, K. A., Khafagy, E.-S., El-damasy, D. A., Hegazy, W. A. H., & Abbas, H. A. (2022). Sodium Citrate Alleviates Virulence in Pseudomonas aeruginosa. Microorganisms, 10(5), 1046. https://doi.org/10.3390/microorganisms10051046

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