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Microorganisms
Volume 10
Issue 7
10.3390/microorganisms10071280
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Article

Arcobacter butzleri Biofilms: Insights into the Genes Beneath Their Formation

by
Adrián Salazar-Sánchez
1,
Itsaso Baztarrika
1,
Rodrigo Alonso
1,2,
Aurora Fernández-Astorga
1,
Ilargi Martínez-Ballesteros
1,2 and
Irati Martinez-Malaxetxebarria
1,2,*
1
MikroIker Research Group, Department of Immunology, Microbiology and Parasitology, Faculty of Pharmacy, University of the Basque Country UPV/EHU, Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Spain
2
Bioaraba, Microbiology, Infectious Disease, Antimicrobial Agents, and Gene Therapy, 01006 Vitoria-Gasteiz, Spain
*
Author to whom correspondence should be addressed.
Microorganisms 2022, 10(7), 1280; https://doi.org/10.3390/microorganisms10071280
Submission received: 15 May 2022 / Revised: 17 June 2022 / Accepted: 22 June 2022 / Published: 23 June 2022
(This article belongs to the Special Issue Host–Biofilm Interactions)
Versions Notes

Abstract

Arcobacter butzleri, the most prevalent species of the genus, has the demonstrated ability to adhere to various surfaces through biofilm production. The biofilm formation capability has been related to the expression of certain genes, which have not been characterized in A. butzleri. In order to increase the knowledge of this foodborne pathogen, the aim of this study was to assess the role of six biofilm-associated genes in campylobacteria (flaA, flaB, fliS, luxS, pta and spoT) in the biofilm formation ability of A. butzleri. Knockout mutants were constructed from different foodborne isolates, and static biofilm assays were conducted on polystyrene (PS), reinforced glass and stainless steel. Additionally, motility and Congo red binding assays were performed. In general, mutants in flaAB, fliS and luxS showed a decrease in the biofilm production irrespective of the surface; mutants in spoT showed an increase on stainless steel, and mutants in pta and spoT showed a decrease on reinforced glass but an increase on PS. Our work sheds light on the biofilm-related pathogenesis of A. butzleri, although future studies are necessary to achieve a satisfactory objective.
Keywords: Arcobacter butzleri; biofilm; knockout mutants; Congo red assay; static biofilm assays Arcobacter butzleri; biofilm; knockout mutants; Congo red assay; static biofilm assays

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MDPI and ACS Style

Salazar-Sánchez, A.; Baztarrika, I.; Alonso, R.; Fernández-Astorga, A.; Martínez-Ballesteros, I.; Martinez-Malaxetxebarria, I. Arcobacter butzleri Biofilms: Insights into the Genes Beneath Their Formation. Microorganisms 2022, 10, 1280. https://doi.org/10.3390/microorganisms10071280

AMA Style

Salazar-Sánchez A, Baztarrika I, Alonso R, Fernández-Astorga A, Martínez-Ballesteros I, Martinez-Malaxetxebarria I. Arcobacter butzleri Biofilms: Insights into the Genes Beneath Their Formation. Microorganisms. 2022; 10(7):1280. https://doi.org/10.3390/microorganisms10071280

Chicago/Turabian Style

Salazar-Sánchez, Adrián, Itsaso Baztarrika, Rodrigo Alonso, Aurora Fernández-Astorga, Ilargi Martínez-Ballesteros, and Irati Martinez-Malaxetxebarria. 2022. "Arcobacter butzleri Biofilms: Insights into the Genes Beneath Their Formation" Microorganisms 10, no. 7: 1280. https://doi.org/10.3390/microorganisms10071280

APA Style

Salazar-Sánchez, A., Baztarrika, I., Alonso, R., Fernández-Astorga, A., Martínez-Ballesteros, I., & Martinez-Malaxetxebarria, I. (2022). Arcobacter butzleri Biofilms: Insights into the Genes Beneath Their Formation. Microorganisms, 10(7), 1280. https://doi.org/10.3390/microorganisms10071280

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