Deciphering Molecular Factors That Affect Electron Transfer at the Cell Surface of Electroactive Bacteria: The Case of OmcA from Shewanella oneidensis MR-1

Louro, Ricardo O.; Rusconi, Giovanni; Fonseca, Bruno M.; Paquete, Catarina M.

doi:10.3390/microorganisms11010079

Open AccessArticle

Deciphering Molecular Factors That Affect Electron Transfer at the Cell Surface of Electroactive Bacteria: The Case of OmcA from Shewanella oneidensis MR-1

by

Ricardo O. Louro

^*

,

Giovanni Rusconi

,

Bruno M. Fonseca

and

Catarina M. Paquete

^*

Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal

^*

Authors to whom correspondence should be addressed.

Microorganisms 2023, 11(1), 79; https://doi.org/10.3390/microorganisms11010079

Submission received: 30 November 2022 / Revised: 23 December 2022 / Accepted: 24 December 2022 / Published: 28 December 2022

(This article belongs to the Special Issue Biological-Electrode Interface as the Nexus of Breakthroughs towards Viability of Microbial Electrochemical Technologies)

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Abstract

Multiheme cytochromes play a central role in extracellular electron transfer, a process that allows microorganisms to sustain their metabolism with external electron acceptors or donors. In Shewanella oneidensis MR-1, the decaheme cytochromes OmcA and MtrC show functional specificity for interaction with soluble and insoluble redox partners. In this work, the capacity of extracellular electron transfer by mutant variants of S. oneidensis MR-1 OmcA was investigated. The results show that amino acid mutations can affect protein stability and alter the redox properties of the protein, without affecting the ability to perform extracellular electron transfer to methyl orange dye or a poised electrode. The results also show that there is a good correlation between the reduction of the dye and the current generated at the electrode for most but not all mutants. This observation opens the door for investigations of the molecular mechanisms of interaction with different electron acceptors to tailor these surface exposed cytochromes towards specific bio-based applications.

Keywords: Shewanella oneidensis MR-1; outer-membrane cytochrome; extracellular respiration; indirect electron transfer; mutagenesis; binding processes

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MDPI and ACS Style

Louro, R.O.; Rusconi, G.; Fonseca, B.M.; Paquete, C.M. Deciphering Molecular Factors That Affect Electron Transfer at the Cell Surface of Electroactive Bacteria: The Case of OmcA from Shewanella oneidensis MR-1. Microorganisms 2023, 11, 79. https://doi.org/10.3390/microorganisms11010079

AMA Style

Louro RO, Rusconi G, Fonseca BM, Paquete CM. Deciphering Molecular Factors That Affect Electron Transfer at the Cell Surface of Electroactive Bacteria: The Case of OmcA from Shewanella oneidensis MR-1. Microorganisms. 2023; 11(1):79. https://doi.org/10.3390/microorganisms11010079

Chicago/Turabian Style

Louro, Ricardo O., Giovanni Rusconi, Bruno M. Fonseca, and Catarina M. Paquete. 2023. "Deciphering Molecular Factors That Affect Electron Transfer at the Cell Surface of Electroactive Bacteria: The Case of OmcA from Shewanella oneidensis MR-1" Microorganisms 11, no. 1: 79. https://doi.org/10.3390/microorganisms11010079

APA Style

Louro, R. O., Rusconi, G., Fonseca, B. M., & Paquete, C. M. (2023). Deciphering Molecular Factors That Affect Electron Transfer at the Cell Surface of Electroactive Bacteria: The Case of OmcA from Shewanella oneidensis MR-1. Microorganisms, 11(1), 79. https://doi.org/10.3390/microorganisms11010079

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Deciphering Molecular Factors That Affect Electron Transfer at the Cell Surface of Electroactive Bacteria: The Case of OmcA from Shewanella oneidensis MR-1

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