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Article
Peer-Review Record

tilS and rpoB: New Molecular Markers for Phylogenetic and Biodiversity Studies of the Genus Thiothrix

Microorganisms 2023, 11(10), 2521; https://doi.org/10.3390/microorganisms11102521
by Nikolai V. Ravin 1, Dmitry D. Smolyakov 2, Nikita D. Markov 2, Alexey V. Beletsky 1, Andrey V. Mardanov 1, Tatyana S. Rudenko 2 and Margarita Yu. Grabovich 2,*
Reviewer 1: Anonymous
Microorganisms 2023, 11(10), 2521; https://doi.org/10.3390/microorganisms11102521
Submission received: 28 August 2023 / Revised: 1 October 2023 / Accepted: 5 October 2023 / Published: 9 October 2023

Round 1

Reviewer 1 Report

The study aims to propose a useful phylogenetic marker (instead of 16S rDNA) for a rapid classification of Thiothrix genus. It is a very interesting and valuable study for experts on this bacterial genus. Aims, methods and conclusions are actual and correct. Therefore, I recommend accepting this manuscript in its current form.

Question: Is there a possibility to use this marker also in a mixture sample, mixed bacterial cultures?

Author Response

Thank you very much for taking the time to review this manuscript. We appreciate your time and labor. Responses to your comments are provided below.

 

 

Comment 1: Is there a possibility to use this marker also in a mixture sample, mixed bacterial cultures?

Response 1: Yes, our study was primarily designed to screen specifically for mixed cultures.

 

Kind regards,

Margarita Grabovich

Reviewer 2 Report

Testing new genetic markers that allow separating species within bacterial genera, provides important tools to further study specific groups of interest, such as the case of Thiothrix. The search for this type of genes seems very relevant to me and in this case very practical.

Author Response

 

Thank you very much for taking the time to review this manuscript. We appreciate your time and labor. We are very grateful for the high assessment of our research.

Kind regards,

Margarita Grabovich

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