Methanomethylophilus alvi gen. nov., sp. nov., a Novel Hydrogenotrophic Methyl-Reducing Methanogenic Archaea of the Order Methanomassiliicoccales Isolated from the Human Gut and Proposal of the Novel Family Methanomethylophilaceae fam. nov.
Abstract
:1. Introduction
2. Materials and Methods
2.1. Medium of Enrichment, Isolation, and Maintenance
2.2. Genome Sequencing, Genomic and Phylogenomic Analyses
2.3. Microscopy
2.4. Physiological Characterization
3. Results and Discussion
3.1. Selective Enrichment and Isolation
3.2. Phylogeny and Genome Features
3.3. Morphology
3.4. Physiology
4. Conclusions
4.1. Description of Methanomethylophilus gen. nov.
4.2. Description of Methanomethylophilus alvi sp. nov.
4.3. Description of Methanomethylophilaceae fam. nov.
5. Patents
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Characteristics | 1 | 2 |
---|---|---|
Source | Human feces | Human feces |
Genome length (bp) | 1,666,767 | 2,637,810 |
DNA G+C content (mol%) | 55.5 | 59.93 |
Morphology | Cocci, as single cells | Cocci, as single cells |
Diameter (µm) | 0.4–0.7 | 0.7–1.0 |
Motility | Non-motile | Non-motile |
Growth temperature | ||
Range | 30–40 | 30–40 |
Optimum | 37 | 37 |
Growth pH | ||
Range | 6.9–8.3 | 7.2–8.4 |
Optimum | 7.5 | 7.6 |
Growth NaCl (mol.L−1) | ||
Range | 0.02–0.34 | 0.02–0.26 |
Optimum | 0.12 | 0.17 |
Required growth factor | Filtrate from a grown E. lenta or rumen fluid | acetate |
Substrates for methane production | H2/methanol H2/monomethylamine H2/dimethylamine H2/trimethylamine | H2/methanol H2/monomethylamine H2/dimethylamine H2/trimethylamine |
Lysed by osmotic shock/SDS | yes | yes |
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Borrel, G.; Fadhlaoui, K.; Ben Hania, W.; Gaci, N.; Pehau-Arnaudet, G.; Chaudhary, P.P.; Vandekerckove, P.; Ballet, N.; Alric, M.; O’Toole, P.W.; et al. Methanomethylophilus alvi gen. nov., sp. nov., a Novel Hydrogenotrophic Methyl-Reducing Methanogenic Archaea of the Order Methanomassiliicoccales Isolated from the Human Gut and Proposal of the Novel Family Methanomethylophilaceae fam. nov. Microorganisms 2023, 11, 2794. https://doi.org/10.3390/microorganisms11112794
Borrel G, Fadhlaoui K, Ben Hania W, Gaci N, Pehau-Arnaudet G, Chaudhary PP, Vandekerckove P, Ballet N, Alric M, O’Toole PW, et al. Methanomethylophilus alvi gen. nov., sp. nov., a Novel Hydrogenotrophic Methyl-Reducing Methanogenic Archaea of the Order Methanomassiliicoccales Isolated from the Human Gut and Proposal of the Novel Family Methanomethylophilaceae fam. nov. Microorganisms. 2023; 11(11):2794. https://doi.org/10.3390/microorganisms11112794
Chicago/Turabian StyleBorrel, Guillaume, Khaled Fadhlaoui, Wajdi Ben Hania, Nadia Gaci, Gérard Pehau-Arnaudet, Prem Prashant Chaudhary, Pascal Vandekerckove, Nathalie Ballet, Monique Alric, Paul William O’Toole, and et al. 2023. "Methanomethylophilus alvi gen. nov., sp. nov., a Novel Hydrogenotrophic Methyl-Reducing Methanogenic Archaea of the Order Methanomassiliicoccales Isolated from the Human Gut and Proposal of the Novel Family Methanomethylophilaceae fam. nov." Microorganisms 11, no. 11: 2794. https://doi.org/10.3390/microorganisms11112794