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Volume 11
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10.3390/microorganisms11081915
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Article

E7 Oncogene HPV58 Variants Detected in Northeast Brazil: Genetic and Functional Analysis

by
Bárbara Simas Chagas
1,†,
Elias Tibúrcio Júnior
1,†,
Ruany Cristyne de Oliveira Silva
1,
Daffany Luana dos Santos
1,
Marconi Rego Barros Junior
1,
Rita de Cássia Pereira de Lima
1,
Maria da Conceição Viana Invenção
1,
Vanessa Emanuelle Pereira Santos
1,
Pedro Luiz França Neto
1,
Antônio Humberto Silva Júnior
2,
Jacinto Costa Silva Neto
3,
Marcus Vinícius de Aragão Batista
4 and
Antonio Carlos de Freitas
1,*
1
Laboratory of Molecular Studies and Experimental Therapy (LEMTE), Department of Genetics, Federal University of Pernambuco, Recife 50670-901, PE, Brazil
2
Center for Biological and Health Sciences, Federal University of Campina Grande, Campina Grande 58429-900, PB, Brazil
3
Laboratory of Molecular and Cytological Research, Department of Histology, Federal University of Pernambuco, Recife 50670-901, PE, Brazil
4
Laboratory of Molecular Genetics and Biotechnology, Department of Biology, Federal University of Sergipe, São Cristóvão 49100-000, SE, Brazil
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Microorganisms 2023, 11(8), 1915; https://doi.org/10.3390/microorganisms11081915
Submission received: 15 June 2023 / Revised: 21 July 2023 / Accepted: 25 July 2023 / Published: 27 July 2023
(This article belongs to the Special Issue Emerging Viruses: New Challenges in Detection, Prevention and Control)
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Abstract

Cervical cancer is associated with persistent infections by high-risk Human Papillomavirus (HPV) types that may have nucleotide polymorphisms and, consequently, different oncogenic potentials. Therefore, this study aimed to evaluate the genetic variability and structural effects of the E7 oncogene of HPV58 in cervical scraping samples from Brazilian women. The study was developed with patients from hospitals in the metropolitan area of Recife, PE, Brazil. The most frequent HPV types were, in descending order of abundance, HPV16, 31, and 58. Phylogenetic analysis demonstrated that the isolates were classified into sublineages A2, C1, and D2. Two positively selected mutations were found in E7: 63G and 64T. The mutations G41R, G63D, and T64A in the E7 protein reduced the stability of the protein structure. Utilizing an NF-kB reporter assay, we observed a decrease in the NK-kB pathway activity with the HPV58-E7 variant 54S compared to the WT E7. The other detected E7 HPV58 variants presented similar NF-kB pathway activity compared to the WT E7. In this study, it was possible to identify mutations that may interfere with the molecular interaction between the viral oncoproteins and host proteins.
Keywords: human papillomavirus 58; E7 oncogene; variants; phylogenetic analysis; gene expression human papillomavirus 58; E7 oncogene; variants; phylogenetic analysis; gene expression

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MDPI and ACS Style

Chagas, B.S.; Tibúrcio Júnior, E.; Silva, R.C.d.O.; Santos, D.L.d.; Barros Junior, M.R.; Lima, R.d.C.P.d.; Invenção, M.d.C.V.; Santos, V.E.P.; França Neto, P.L.; Silva Júnior, A.H.; et al. E7 Oncogene HPV58 Variants Detected in Northeast Brazil: Genetic and Functional Analysis. Microorganisms 2023, 11, 1915. https://doi.org/10.3390/microorganisms11081915

AMA Style

Chagas BS, Tibúrcio Júnior E, Silva RCdO, Santos DLd, Barros Junior MR, Lima RdCPd, Invenção MdCV, Santos VEP, França Neto PL, Silva Júnior AH, et al. E7 Oncogene HPV58 Variants Detected in Northeast Brazil: Genetic and Functional Analysis. Microorganisms. 2023; 11(8):1915. https://doi.org/10.3390/microorganisms11081915

Chicago/Turabian Style

Chagas, Bárbara Simas, Elias Tibúrcio Júnior, Ruany Cristyne de Oliveira Silva, Daffany Luana dos Santos, Marconi Rego Barros Junior, Rita de Cássia Pereira de Lima, Maria da Conceição Viana Invenção, Vanessa Emanuelle Pereira Santos, Pedro Luiz França Neto, Antônio Humberto Silva Júnior, and et al. 2023. "E7 Oncogene HPV58 Variants Detected in Northeast Brazil: Genetic and Functional Analysis" Microorganisms 11, no. 8: 1915. https://doi.org/10.3390/microorganisms11081915

APA Style

Chagas, B. S., Tibúrcio Júnior, E., Silva, R. C. d. O., Santos, D. L. d., Barros Junior, M. R., Lima, R. d. C. P. d., Invenção, M. d. C. V., Santos, V. E. P., França Neto, P. L., Silva Júnior, A. H., Silva Neto, J. C., Batista, M. V. d. A., & Freitas, A. C. d. (2023). E7 Oncogene HPV58 Variants Detected in Northeast Brazil: Genetic and Functional Analysis. Microorganisms, 11(8), 1915. https://doi.org/10.3390/microorganisms11081915

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