1. Introduction
The bullfrog
Aquarana catesbeiana was first introduced into mainland China from Cuba in 1962 as a food source due to its high protein provision, soft texture, ease of farming, and high economic value. Since 2000, the bullfrog farming industry in China has experienced rapid growth [
1]. In 2021, the production of bullfrogs had reached 600,000 tons, with an economic value of USD 11.2 billion [
2]. However, the overall survival rate of farmed bullfrogs from their hatching to harvest is approximately 10%, as bullfrog tadpoles are fragile during the metamorphosis period.
During the metamorphosis stage, the bullfrog tadpoles undergo significant changes in their internal organs, drastically transforming from aquatic larvae to terrestrial adults. According to Gosner’s research [
3], the hind limb bud is absent in the larvae at the G25 stage. The metatarsal tubercle appears in the base of the first toe at the G38 stage. Forelimbs emerge at the G42 stage, which is the metamorphic climax. Complete metamorphosis occurs at the G46 stage, when the animal fully develops its limbs and resorbs its tail. During the bullfrog–tadpole metamorphosis, the tadpole first resorbs its tail and gills, which are then replaced by fully developed external appendages and lungs to adapt to a terrestrial lifestyle. As a result, their diets change significantly, with the larvae preferring plant material while the adults are mainly carnivorous. Due to their omnivorous preference, the larvae have evolved a diverse intestinal morphology, ranging from a long, double–spiral intestine to a short intestine in adults [
4].
The intestinal microbiota of bullfrogs plays a crucial role in maintaining bullfrogs’ health and undergoes a complex interplay during their metamorphosis [
5,
6]. From birth, the microbiota co–develops with the host and aids in enhancing the epithelium, regulating immune responses, modulating the energy balance, and influencing the development and behavior of the host [
7]. However, the diversity and composition of the microbial community are affected by the host’s genome, nutrition, and lifestyle. Despite this, there are limited studies on bullfrog tadpoles during metamorphosis, which makes it crucial to understand the shifts in the intestinal bacterial community during this process.
The main objective of this study was to determine the diversity and composition of intestinal bacteria in A. catesbeiana during bullfrog–tadpole metamorphosis by sequencing the 16S rRNA gene. This study also aimed to investigate the changes in morphological traits and intestinal histology during this process. The results of this study will be crucial to understanding the transition of gut microbial communities in the bullfrog’s life cycle. Additionally, the findings will aid in selecting potential probiotics to enhance the metamorphosis rate.
2. Material and Methods
2.1. Animals
The bullfrog
A. catesbeiana specimens were raised at the Guangzhou Shengshi Tangfeng Fishery Co., Ltd. (Guangzhou, China). The embryos were obtained from sexually mature frogs by natural oviposition in March and allowed to hatch in nylon mesh tanks (80 cm × 80 cm × 70 cm, 80 μm) with water at a 30 cm depth without any manipulation. The tadpoles were reared from the embryos and they underwent metamorphic development in plastic tanks (3 m × 5 m × 0.6 m, water depth 0.3 m) using a recirculating aquaculture system. All the larvae were fed identically based on their developmental stages. The determination of the developmental stages of the larvae of
A. catesbeiana was conducted based on Gosner’s studies [
3]. Larvae at the G25 (the hind limb bud is not present yet), G38 (the metatarsal tubercle emerges at the base of the first toe), G42 (forelimbs appear), and G46 (the tail is completely resorbed and the limbs are fully developed) stages were collected. All the animals were starved for one day before measuring morphology and collecting gut samples.
The procedures for collecting and handling the animals were strictly followed, and were provided by the Institution Animal Care and Committee on Laboratory Animal Welfare and Ethics of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (SCSFRI–CAFS, No. nhdf2024–12) and were consistent with China’s Animal Welfare Legislation guidelines.
2.2. Morphology Measurements
Ten larvae were collected randomly from four developmental groups (G25, G38, G42, and G46) to study the morphological changes over the metamorphic stages after being starved for one day. The total body mass (TBM) was measured using an electronic analytical balance, while the total length (TOL), the snout–vent length (SVL), and the body width (BW) were measured as described by Fei et al. [
8].
2.3. Intestinal Histological Processing
The jejunum samples from 12 tadpoles (G25, G38, G42, and G46, n = 3 per stage) were collected to analyze the intestine’s morphological and histological changes during the metamorphosis developmental process. The samples were preserved in 4% formaldehyde and processed for hematoxylin and eosin (H&E) staining. The H&E section and light microscopy images were prepared by Wuhan Sevicebio Technology Co., Ltd. (Wuhan, China).
2.4. 16S rRNA Gene Sequencing
Six biological replicates were conducted in each critical developmental group (G25, G38, G42, and G46). In each replicate, three intestines were pooled. The intestine was excised intact from the posterior of the esophagus to the vent, rinsed three times with sterile water, and stored in a 2 mL sterile centrifuge tube. It was immediately frozen in liquid nitrogen and kept at −80 °C for DNA extraction and bacterial community analysis.
The bacterial DNA extraction, PCR amplification, and Illumina NovaSeq sequencing were carried out by Microeco Technology Co., Ltd. in Shenzhen, China. The following steps were followed: First, the total genomic DNA was extracted from the intestinal content samples using the CTAB method. Next, the V3–V4 variable region was PCR amplified with a barcoded fusion primer pair, 341F (5′–CCTAYGGGRBGCAS–CAG–3′) and 806R (5′–GGACTACNNGGGTATCTAAT–3′). Then, the original sequence of all the samples was quality controlled, denoised, spliced, and decimalized using the DADA2 plugin in Qiime2 software to form Operational Taxonomic Units (OTUs). Finally, the OTUs were compared with the Greengenes Database (version 13.8) to obtain species annotation information.
Various methods, including ANCOM, ANOVA, Kruskal–Wallis, LEfSe, and DEseq2, were employed to identify the bacteria, with varying abundances among the samples and groups [
9]. The α– and β diversity indices were calculated using the core–diversity plugin within QIIME2 [
10]. PLS–DA (partial least squares discriminant analysis) was also used as a supervised model to reveal the microbiota variation among groups, using the “plsda” function in the R package “mixOmics” [
11]. Redundancy analysis (RDA) was performed to reveal the association of microbial communities with environmental factors based on the relative abundances of microbial species at different taxa levels using the R package “vegan” [
12]. Co–occurrence analysis was performed by calculating Spearman’s rank correlations between the predominant taxa, and the network plot was used to display the associations among taxa. In addition, the potential KEGG Ortholog (KO) functional profiles of microbial communities were predicted with PICRUSt [
13]. Unless specified above, the parameters used in the analysis were set at default settings. All data analysis was processed using the online platform Wekemo Bioincloud (
https://www.bioincloud.tech) (accessed on 1 September 2024).
2.5. Statistical Analysis
The data are presented as the mean ± standard deviation, and statistical analysis was performed using one–way ANOVA (SPSS for Windows, Version 22.0) followed by post hoc Duncan multiple range tests to determine the significant differences between four different, developmental stage groups. A p–value of <0.05 was considered statistically significant. The results were presented using GraphPad Prism software (version 7, GraphPad Software, Inc., San Diego, CA, USA) and included all biological repeats and the level of significant difference.
4. Discussion
Throughout the process of tadpoles’ transformation into young frogs,
A. catesbeiana tadpoles undergo significant physical and physiological changes, as well as a shift in diet from primarily plant–based to carnivorous. The morphological measurements taken in this study demonstrated a significant reduction in the total length and snout–vent length of
A. catesbeiana during metamorphosis. Similar results were observed in
Rana chensinensis tadpoles undergoing metamorphosis [
14]. These findings suggest that all the changes observed in anuran amphibians are adaptations necessary for transitioning from their aquatic habitat to a terrestrial one.
Intestinal epithelial cells are a crucial component of the intestinal epithelium, where they perform essential functions such as digesting food, absorbing nutrients, and protecting the host from microbial infections [
15]. At stage G46, the height of the jejunum epithelial cells was significantly higher than that at stage G38, and more mucosal folds were present. The mucosal folds at stage G46 were longer, thicker, and more complex, with curly branches, which is consistent with the observation in
R. temporaria tadpoles [
16]. Moreover, the number of goblet cells increased during stages 42–46, indicating that the goblet cells adjusted to the changes in dietary habits as the
A. catesbeiana tadpoles developed.
Stage G46 was found to be separated from the other three stages in the NMDS space, indicating that the G25, G38, and G42 groups shared similar intestinal microbiota. Our research illustrated that the composition of the intestinal microorganisms differed significantly at different developmental stages throughout the metamorphosis of
A. catesbeiana, which is consistent with the Chinese brown frog (
R. chensinensis) [
14] and the Northern leopard frog (
Lithobates pipiens) [
17]. The G25, G38, and G42 stages take place during the aquatic stage, while stage G46 occurs during the terrestrial stage, so that the quite different microorganisms in stage G46 correlate well with the lifestyle transformation of becoming land–based. The structural changes in intestinal microorganisms observed in our study are likely due to the biological transformations occurring during metamorphosis and the associated lifestyle shift. As metamorphosis induces significant physiological, anatomical, and ecological changes, these transitions reshape the host’s environment, driving shifts in the gut microbiota. Our findings indicate that post–metamorphosis, the microbiota adapt to reflect the host’s evolving nutritional and environmental demands.
This observation is consistent with previous studies, such as Warne et al. [
18], which demonstrated that gut microbiota manipulation during critical developmental windows impacts host physiology and disease susceptibility. The dynamic interaction between microbiota and host development suggests microbial shifts not only respond to environmental changes, but also influence physiological adaptations. These shifts, driven by metamorphosis, may enhance the host’s survival and performance in the post–metamorphic stage.
Thus, our hypothesis aligns with Warne et al. [
18], supporting a bidirectional relationship between host development and microbiota. Further studies are needed to determine whether specific microbial changes directly affect growth, immunity, and disease resistance, providing insights into gut health management during critical developmental phases.
The G25, G38, and G42 groups exhibited a community dominated by the phyla
Fusobacteria and
Bacteroidetes, while the G46 group maintained a community rich in
Firmicutes. The presence of
Fusobacteria was higher in the stage G25, G38, and G42 groups than in the G46 group. This trend was observed in the gut of several fish species, such as
Lepomis macrochirus (82.60%),
Micropterus salmoides (90.60%) and
Ictalurus punctatus (94.90%) [
19]. In comparative studies of the intestinal microbiota of different mammals, it was found that marine mammals had a considerably higher abundance of
Fusobacteria than terrestrial mammals [
20]. However, while the abundance of
Fusobacteria detected was at low levels in the gut microbiota of Northern leopard frog adults (0.32%) [
17], it was almost completely absent in tadpoles (<0.01%), suggesting different frog species may have different microbiota Hence, the function of
Fusobacteria in aquatic and terrestrial animals needs to be explored further.
It was found that the
Bacteroidetes phylum was more abundant in terrestrial animals (34.00–39.40%) when compared to aquatic fish samples (4.85–19.03%) [
21,
22,
23]. Interestingly, the presence of a
Bacteroidetes–rich gut community was observed in adult frogs of
L. pipiens (22.82%) but not in tadpoles (2.43%) [
17]. The pre–metamorphosis process (G25, G38 and G42) of
A. catesbeiana is the aquatic stage, while the metamorphosis process (G46) of
A. catesbeiana is the terrestrial stage. In the present study, it was observed that the abundance of
Bacteroidetes was higher in the pre–metamorphosis process (G25, G38, and G42, 7.46–28.83%) compared to the metamorphosis process (G46, 1.06%), which is contrary to the observation made in
L. pipiens.
During the G46 stage, the number of
Bacteroidetes decreased in
A. catesbeiana bullfrogs, which
Firmicutes replaced. This change resulted in a higher ratio of
Firmicutes–to–
Bacteroidetes, which could enhance the efficiency of calorie uptake from the food they consume [
24]. The major components of these
Firmicutes were
Weissella (28.20%) and
Clostridium (22.65%), which are believed to support digestion and nutrient acquisition during the G46 metamorphosis stage, though further investigation is required to determine the exact role of
Weissella and
Clostridium. Nonetheless, the increased
Firmicutes–to–
Bacteroidetes ratio suggests that metamorphosis and lifestyle changes can significantly impact the microbial community in
A. catesbeiana bullfrogs.
According to the RDA analyses and Spearman correlation, changes in the morphology of the host have distinct effects on the bacterial community in the intestine of
A. catesbeiana. The bacteria related to
Prevotella,
Bifidobacterium,
Leucobacter,
Corynebacterium, and
Bulleidia were all found to be positively correlated with the total body mass. In contrast, the
Dorea,
Robinsoniella, and
Clostridium genera were positively correlated with the epithelial cell height. The measured factors of both the epithelial cell height and the total body mass were found to be clustered together, indicating that the intestinal epithelial cells play a vital role in the digestion of food and absorption of nutrients, thereby contributing to increasing the host’s total body mass [
25]. Furthermore, the increase in total body mass was accompanied by a simultaneous increase in the
Prevotella,
Bifidobacterium,
Leucobacter,
Corynebacterium, and
Bulleidia genera. On the other hand, an increase in the genera of
Dorea,
Robinsoniella, and
Clostridium was accompanied by an increase in epithelial cell height. These bacterial genera have the potential to be developed as probiotics in the future.