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Article

Virulence and Antimicrobial Resistance of Listeria monocytogenes Isolated from Ready-to-Eat Food Products in Romania

by
Mihaela Niculina Duma
1,
Laurenţiu Mihai Ciupescu
2,
Sorin Daniel Dan
3,
Oana Lucia Crisan-Reget
3 and
Alexandra Tabaran
3,*
1
Laboratory of Food Microbiology, Sanitary Veterinary Directorate for Food Safety, 400621 Cluj-Napoca, Romania
2
The Institute of Hygiene and Veterinary Public, The National Sanitary Veterinary Authority for Food Safety, Campul Mosilor 5, 013701 Bucharest, Romania
3
Department of Animal Husbandry and Public Health, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, Romania
*
Author to whom correspondence should be addressed.
Microorganisms 2024, 12(5), 954; https://doi.org/10.3390/microorganisms12050954
Submission received: 29 March 2024 / Revised: 25 April 2024 / Accepted: 27 April 2024 / Published: 8 May 2024
(This article belongs to the Special Issue Advances in Antibiotic and Antifungal Resistance and Related Alternative Therapies, Second Edition)
Versions Notes

Abstract

:
Listeria monocytogenes (L. monocytogenes) poses a significant threat to food safety due to its ability to cause severe human illness and its resistance to various antibiotics and environmental conditions. This study investigated the prevalence, serotype distribution, virulence gene profiles, and antimicrobial resistance patterns of L. monocytogenes in ready-to-eat (RTE) food products from Romania. A total of 8151 samples were analyzed, including various processed dairy, bovine, poultry, pork, and fish products. Bacterial isolation was conducted using the classical standard method, followed by confirmation through biochemical and molecular testing. Among the isolated strains, serotypes 1/2a, 1/2b, and 1/2c were identified, with a prevalence of 75% for serotype 1/2a. Additionally, virulence genes specific to listeriolysin O (hlyA) and regulatory factor A (prfA) were detected in all isolates. Antimicrobial susceptibility testing revealed varying resistance patterns among the L. monocytogenes strains. Trimethoprim-sulfamethoxazole and oxacillin showed the highest prevalence of resistance at 26.92% and 23.07%, respectively. However, all strains remained susceptible to ciprofloxacin, levofloxacin, and moxifloxacin. Notably, 23.07% of the isolates exhibited multidrug resistance, with the most common pattern being resistance to oxacillin, penicillin, and tetracycline. Analysis of antimicrobial resistance genes identified tetracycline resistance genes, particularly tet(C), tet(M), and tet(K), in a significant proportion of isolates. The presence of ampC and dfrD genes was also notable, indicating potential mechanisms of resistance. These results emphasize the necessity for ongoing surveillance of L. monocytogenes in RTE foods and emphasize the importance of thorough monitoring of antimicrobial resistance to guide public health strategies within the European Union.

1. Introduction

Listerias, a group of bacteria within the Clostridium phylogenetic branch and Listeriaceae family, comprises a genus with 26 species, including 18 Listeria species recognized and described for the first time in 2009. Based on genetic and observable traits, a distinct group of six species, known as Listeria sensu strictu, share common features like thriving in low temperatures and having flagellar motility, with the pathogenic Listeria monocytogenes being part of this group. The remaining species, termed Listeria sensu lato, form three separate phylogenetic groups, suggesting they could be recognized as distinct genera. These proposed genera typically lack pathogenicity, are often non-motile (except for Listeria grayi), possess nitrate reduction abilities (except Listeria floridensis), and yield negative results in the Voges–Proskauer test (except L. grayi) [1].
Human pathogens are of particular concern to health authorities. While the incidence of listeriosis is lower compared to other foodborne zoonoses, it remains worrisome due to its severity and resilience, making eradication challenging [2]. Various strains are resistant to multiple classes of antibiotics, and many L. monocytogenes strains can withstand chemical treatments, such as disinfectants [3]. Consequently, combating L. monocytogenes contamination in food processing facilities is difficult, as it can form biofilms on various surfaces and survive and multiply under extreme environmental conditions, such as low temperatures, wide pH ranges, and high salt concentrations [4].
The rare but severe consequences of human food contamination by L. monocytogenes, and to a lesser extent by L. ivanovii, justify the implementation of monitoring these bacteria in the main food processing sectors, particularly in animal-derived products [5].
The pathogenicity of L. monocytogenes is attributed to the invasion of host cells by several virulence factors. Numerous studies highlight the role of extracellular listeriolysin O, which regulates host cell functions [6]. This pore-forming protein, encoded by the hly gene, is crucial for the bacterium’s interaction with the host cell. Other virulence-associated genes include actA, responsible for actin polymerization and mobility, plcA and plcB, involved in membrane lysis during cell propagation, and inlA, facilitating bacterial invasion into intestinal epithelial cells [7,8].
Surveillance of Listeria’s presence is crucial because it allows, among others, the identification and characterization of specific serotypes, such as 1/2a, 1/2b, and 4b, which are responsible for the majority of human listeriosis cases [9].
In 2022, listeriosis ranked as the fifth most frequently reported zoonosis in the EU, with 2738 cases—a 15.9% increase in the EU notification rate compared to 2021 [10].
Listeria spp. typically demonstrate susceptibility to a broad array of antimicrobials; however, the emergence of the first multi-resistant L. monocytogenes strain dates back to 1988 [11]. Since then, instances of antibiotic-resistant L. monocytogenes isolates have been identified in food, environmental, and human listeriosis cases [11]. Currently, the recommended therapy for human listeriosis involves a combination of a β-lactam antibiotic (such as ampicillin or penicillin) with an aminoglycoside (such as gentamicin), while alternative treatment options include vancomycin, erythromycin, and trimethoprim-sulfamethoxazole for pregnant women or patients with β-lactam allergies [12]. The prevalence and patterns of resistance are influenced by antibiotic utilization practices and regional disparities. Hence, the investigation and surveillance of Listeria spp. antibiotic susceptibility across different geographical regions is crucial for safeguarding public health [13]. Due to these factors, L. monocytogenes represents a considerable threat to the food sector, notably RTE food manufacturers, with meat products remaining among the top three RTE food categories commonly linked to human listeriosis [14].
The culinary practices in Romania are characterized by a strong inclination towards consuming raw or undercooked pork delicacies like ham, sausages, bacon, rillettes, and marinated loin, often prepared at home using fresh meat purchased from retail outlets. These traditional methods of pork consumption carry a heightened risk of contamination with foodborne pathogens. Multidrug-resistant strains of L. monocytogenes, for instance, can potentially infiltrate the food supply chain via raw meat products, posing a significant threat of severe illness in humans. An investigation into antimicrobial susceptibility was conducted on 26 L. monocytogenes isolates obtained from severe clinical cases of listeriosis in Romania [15]. The study revealed a notable proportion (82%) of isolates exhibiting resistance to at least one antibiotic, though no instances of multidrug resistance were observed. Notably, 18 of the examined strains displayed resistance to ciprofloxacin [15]. However, the authors of the conducted study [15] acknowledged the methodological constraint of the investigation, particularly the absence of serotyping of the L. monocytogenes isolates. As a result, critical questions regarding the prevalence and distribution of clinically relevant serotypes, such as 1/2a, 1/2b, 1/2c, and 4b, remained unanswered. Therefore, there is a lack of updated data on the prevalence of L. monocytogenes in Romanian RTE food products and the characterization of virulence, serotype, and antimicrobial resistance. This study aimed to assess the presence of L. monocytogenes in various food matrices collected in the northwestern region of Romania and characterize the virulence, serotype, and antibiotic susceptibility profiles of isolated strains using classical and molecular methods.

2. Materials and Methods

2.1. Sample Collection

The research involved examining 8151 RTE food items received by the Sanitary Veterinary Food Safety Laboratory from the northwestern region of Romania. These samples were analyzed to verify compliance with safety standards, specifically the absence of Listeria spp. in RTE meat products. The samples tested in this food safety laboratory encompassed processed dairy products, bovine meat products, poultry meat products, processed pork meat (such as sausages, ham, and bacon), and fish products. The study comprised the period 2019–2022, during which time the following number of samples were tested: 2019—n = 2178; 2020—n = 2009; 2021—n = 1930; 2022—n = 2034. All the samples were RTE meat products brought directly to the laboratory by the producers in their natural package and in refrigerated storage conditions (0–4 °C).

2.2. Bacterial Isolation

The bacterial isolation protocol was performed according to the horizontal detection and counting method for L. monocytogenes [16]. Briefly, 25 g of each sample investigated was inoculated in 225 mL of selective supplement half Fraser broth (Sharlau, Sentmenat, Spain) and then incubated for 25 ± 1 h at 30 ± 1 °C. Afterward, a second enrichment was performed, which consisted of adding 0.1 mL of the broth culture in 10 mL of full-strength Fraser broth (UVM II Selective Supplement Scharlau/Spain) and incubation at 37 °C for 24 ± 2 h. A loopful of each of the half-and full-strength Fraser broths was plated on the chromogenic agar ALOA (Scharlau/Spain) and Oxford agar (Merck, Darmstadt, Germany). All the plates were incubated in aerobic conditions at 37 °C for 24–48 h.
Specific colonies developed on ALOA and Oxford agar were then re-streaked on tryptic soy agar supplemented with 0.6% yeast extract (TSA-YE) (BioLife, Monza, Italy). The incubation was performed at 37 °C for 24 h. The colonies developed on TSA-YE media were confirmed by Gram Staining, Hemolysis tests on blood agar (Columbia Blood Agar Base Oxoid/Basingstoke, UK + Defribinated Horse Blood), carbohydrate utilization test (Carbohydrates Utilisation Broth Base ISO Condalab/Madrid, Spain), CAMP test (reference strain Staphylococcus aureus ATCC 6538P lot 827-392-3; Rodococcus equi ATCC 6939 lot 697-78-6; L. monocytogenes ATCC 13932 lot 129-101-81; L. ivanovii ATCC 19119 lot 815-66-5; L. innocua ATCC 33090 lot 814-182-2). The confirmation of Listeria monocytogenes was also performed by biochemical testing on the VITEK 2 GP Immunodiagnostic Assay System (Biomerieux/Craponne, France) according to the manufacturer’s instructions.

2.3. Susceptibility Testing

After isolation, the frozen L. monocytogenes strains were thawed and then plated onto Brain Heart Infusion agar (Merck, Germany) before being incubated at 37 °C for 24 h. For antibiotic susceptibility testing, the disc diffusion method was applied, following the standard protocol recommended by the European Committee on Antimicrobial Susceptibility Testing (EUCAST). Briefly, 5–6 colonies from overnight cultures were suspended in 1 mL of 0.9% NaCl solution, and the turbidity was adjusted to a 0.5 McFarland standard. Afterwards, the suspension was inoculated onto Mueller–Hinton agar (Merck, Germany). Antibiotic discs were placed on the agar surface at intervals of 3 cm, each disc containing a specific antibiotic concentration: ampicillin (10 μg), cephalothin (30 μg), ciprofloxacin (5 μg), clindamycin (2 μg), chloramphenicol (30 μg), gentamicin (10 μg), levofloxacin (5 μg), moxifloxacin (5 μg), meticillin (5 μg), oxacillin (1 μg), penicillin G (10 μg), rifampicin (30 μg), trimethoprim-sulfamethoxazole (1.5/23.5 μg), tetracycline (30 μg), and vancomycin (30 μg). Subsequently, the plates were incubated at 37 °C for 24 h. The diameter of the inhibition zones was measured to the nearest millimeter. Interpretation of the data was conducted following the EUCAST criteria [17]. In cases where the EUCAST guidelines did not provide resistance criteria for Listeria, any missing breakpoints were supplemented with those recommended for Staphylococcus aureus and Enterococcus spp. according to CLSI standards [18].

2.4. DNA Extraction from Colonies

The bacterial DNA was extracted using InstaGene Matrix (BIO-RAD, 732-6030, South Granville, Australia), according to the manufacturer protocol, with few modifications. Briefly, two colonies were resuspended in 150 µL of 6% w/v Chelex resin solution (Merck, Germany), followed by incubation at 56 °C for 20 min and 1500 vibrations/min on thermomixer and then the tubes were placed at 98 °C for 15 min. The samples were later centrifuged at 12,000 rpm for 5 min, and the supernatant was stored at −18 °C until use.

2.5. Polymerase Chain Reaction (PCR) for Serotype Testing

The multiplex PCR was performed through the use of primers described by D’Agostino et al. (2004) [19] and Doumith et al. (2004) [20] (Table S1—Supplementary File). The 25 µL PCR reaction consisted of 2.5 µL bacterial DNA, 12.5 µL of 2× QIAGEN Multiplex PCR Master Mix (Qiagen, Hilden, Germany), and 10 µL of primers mix formed by 0.4 µM (forward and reverse primers) for lmo1118, 0.4 µM for lmo0737, orf 2819 and orf 2110, 0.1 µM for prs and 0.2 µM for prfA. The multiplex PCR comprised a step for pre-denaturation and polymerase activation at 95 °C for 5 min, a step of 40 amplification cycles (denaturation at 95 °C for 20 s, hybridization at 54 °C for 40 s, and elongation at 72 °C for 90 s) followed by a final step at 72 °C for 7 min for elongation. The electrophoresis gel was prepared from 2% agarose (Bioline, London, UK) in TAE (Bioline, UK). Electrophoresis was performed for 1 h and 30 min at 100 V, using 10 µL of DNA ladder (Promega, Southampton, UK) and 10 µL of amplicons, all of them mixed with 2 µL of 6x dual-action nontoxic fluorescent nucleic acid stain and loading dye (RedSafe, Bioline, UK). The expected bands for each gene identification are detailed in Table S1 (Supplementary File).

2.6. Polymerase Chain Reaction (PCR) for Virulence Testing

For virulence testing, a multiplex PCR was performed through the use of primers described in Table S2 (Supplementary File). The protocol followed the steps previously described for serotype testing. The L. monocytogenes strain ATCC 19112 was used as a positive reference for the interpretation in the PCR method.

2.7. Polymerase Chain Reaction (PCR) for Antimicrobial Resistance Genes Testing

The molecular method was used to detect the genes conferring streptomycin, chloramphenicol, trimethoprim, ß-lactam, and tetracycline resistance. Primers and temperature conditions for amplification are shown in Table S3 (Supplementary File).

2.8. Statistical Analysis

The statistical analysis was conducted using OriginPro 8.5 software (OriginLab Corporation, Northampton, MA, USA) for the Chi-squared test. A significance level of p ≤ 0.05 was applied for result interpretation. Additionally, the Tukey post hoc test was used to evaluate the statistically significant variances between the resistant and intermediate-resistant L. monocytogenes isolates.

3. Results

3.1. Prevalence, Serotype, and Virulence of L. monocytogenes in the RTE Food Products

Twenty-six isolates of L. monocytogenes were found from the total number of samples investigated in this study (n = 8151). The RTE food products that tested positive were mainly originating from pork meat and fish meat. Taking into consideration the timeframe in which they were isolated, we found that the majority of the strains (n = 21) were isolated during the year 2022. In 2019 and 2021, only one sample was found positive for the presence of L. monocytogenes, while in 2020, three samples were positive. The difference in the number of isolates cannot be attributed to the number of samples investigated, taking into account that in each year, the approximately same number (n = 2178, 2019; n = 2009, 2020; n = 1930, 2021; n = 2034, 2022) was taken into examination. Isolates were assigned to serotypes 1/2a, 1/2b, and 1/2c, with a higher prevalence for 1/2a serotypes (75%). Six serogroup determinants were investigated in our study, prs being the most prevalent and detected in all samples. prfA was also a characteristic virulence gene in all the samples belonging to the 1/2a serotype. Two of the 1/2a serotype isolates tested positive also for orf 2819 (Figure S1, Supplementary File).
A total number of five serotypes were detected in this study (Figure S1, Supplementary File) that had a distinctive pattern of virulence also. All the isolates tested positive for the virulence genes specific to listeriolysin O (hlyA) and for the gene-specific regulatory factor A (prfA) (Table 1, Figure S1 Supplementary File). We found that all of the strains isolated in 2022 (n = 21) tested positive for all the virulence genes investigated. The strain isolated in 2021 also showed positivity to the internalin (inlJ) gene, while the strains isolated in 2019 and 2020 tested positive only for LIPI-1 genes (prfA and hlyA).

3.2. Susceptibility to Antibiotics in L. monocytogenes Isolated in RTE Products

This study aimed to assess the antibiotic resistance profiles of 26 L. monocytogenes strains isolated from RTE products available in the Romanian market. Among the antibiotics tested, trimethoprim-sulfamethoxazole exhibited the highest prevalence of resistance (26.92%), followed by oxacillin (23.07%) (Table 2). Interestingly, all tested strains showed susceptibility to ciprofloxacin, levofloxacin, and moxifloxacin. Additionally, intermediate resistance was observed in a minority of strains to ampicillin (7.69%), meticillin (3.94%), penicillin G (3.94%), and oxacillin (23.07%). The statistical analysis demonstrated significant differences (p < 0.01) in prevalence between the resistant strain isolates and the intermediate ones.
It is important to note that intermediate resistance indicates that while the antibiotic may still have efficacy, its dosage might need adjustment for optimal effectiveness. Notably, 23.07% of the L. monocytogenes strains exhibited multidrug resistance (MDR), defined as resistance to three or more antibiotics. The most prevalent multidrug resistance pattern observed was resistance to oxacillin, penicillin, and tetracycline (Table 3).

3.3. Antimicrobial Resistance Gene Presence in L. monocytogenes

All L. monocytogenes isolates exhibited the presence of various antimicrobial resistance gene sequences. The predominant resistance gene was associated with tetracycline, with twenty out of 26 isolates testing positive for at least one of the relevant genes. The tet(C) genetic determinant was identified in 63% of tetracycline-resistant isolates, followed by tet(M) (42%) and tet(K) (42%). Conversely, other tetracycline resistance genes (tet(A), tet(B), tet(L), and tet(S) were absent in all tested L. monocytogenes isolates from the food products. None of the isolates tested positive for aad6 and cat genes; however, a considerable proportion of L. monocytogenes isolates were positive for ampC (58%) and dfrD (50%) (Table 1).

4. Discussion

A study published by EFSA in 2013 and carried out between 2010 and 2011 showed that the prevalence of L. monocytogenes varied among different categories of RTE food sampled across the European Union. Specifically, the prevalence rates at the end of the shelf-life were 10.3% for smoked or graved fish, 2.07% for packaged heat-treated meat products, and 0.47% for soft or semi-soft cheese products [21]. Our research has shown that the prevalence in Romania during our four-year interval (2019–2022) is 0.31% in the RTE products investigated. The highest prevalence of the total number found positive (n = 26) was in pork meat-originating products (42.3%), and the highest number of positive samples was seen in 2022 (n = 21). It is plausible that the observed increase in positive samples for L. monocytogenes in 2022 could be attributed to various factors, including the impact of the COVID-19 pandemic and changes in production and consumption patterns post-pandemic. During the COVID-19 pandemic, there were disruptions in food production and distribution systems due to lockdowns, restrictions on movement, and workforce shortages. These disruptions could have affected food safety measures and increased the likelihood of contamination or cross-contamination in food processing facilities. Additionally, changes in consumer behavior, such as increased reliance on RTE foods or shifts in dietary preferences, might have influenced the prevalence of L. monocytogenes contamination in certain food products. However, without specific data on the impact of the COVID-19 pandemic on food safety practices and L. monocytogenes contamination rates, it is challenging to definitively attribute the increase in positive samples solely to pandemic-related factors.
In 2011, a separate investigation assessing the prevalence of Listeria spp. in foodstuffs from the southern region of Romania demonstrated a higher prevalence for L. innocua compared to L. monocytogenes [22]. However, it highlights the increase in the contamination of foodstuffs by L. monocytogenes and the resulting significant economic problem, specifying that the presence of strains of Listeria spp. proved to be a useful indicator during all stages of the food production chain [23,24,25,26,27]. Between 2016 and 2019, Romania registered increasing trends in human listeriosis cases, with nine confirmed cases in 2016 and 17 cases in 2019 [14]. However, data concerning the prevalence and antibiotic resistance profiles of strains isolated from RTE products in Romania are lacking. The increased prevalence of L. monocytogenes isolates in 2022 compared to previous years suggests a potential need for heightened surveillance and control measures to prevent further contamination and mitigate public health risks.
Coroneo et al. (2016) [28] documented similar findings in L. monocytogenes isolates from cheese in Italy, observing variable rates of virulence gene detection. Furthermore, other investigations have reported comparable results in samples obtained from various food sources, including raw milk, milking equipment, worker’s hands, and clinical specimens [29]. Similar to our findings, other research revealed the presence of virulence genes in all examined L. monocytogenes isolates isolated from diverse food types [30,31,32,33]. It is recognized that certain polymorphisms and point mutations present in specific virulence genes may contribute to attenuated virulence in L. monocytogenes strains [34,35]. Consequently, the absence or presence of virulence factors could serve as a tool not only to assess risks associated with food consumption but also those linked to strain-specific virulence parameters of L. monocytogenes [36]. Our results revealed that twenty-one strains tested positive for all investigated virulence factors (Table 1). The pathogenicity of L. monocytogenes is governed by several virulence factors, notably the family of internalins, which are bacterial surface proteins responsible for the internalization (entry) of L. monocytogenes into cells (InlA, InlB) [37], and dissemination between cells (InlC) [38]. Another critical factor is listeriolysin O (LLO), a protein facilitating the survival and intracellular multiplication of this pathogen by enabling bacterial escape from the phagosome into the cytoplasm of infected host cells [39].
The identified virulence factors in the context of public health risk underscore the potential severity of L. monocytogenes infections associated with RTE food products, particularly those originating from pork and fish meat. The predominance of serotype 1/2a, which accounted for 75% of isolates, suggests a higher propensity for virulence among these strains. Furthermore, the detection of key virulence genes such as listeriolysin O (hlyA), regulatory factor A (prfA), and internalin (inlJ) across multiple serotypes highlights the widespread presence of virulence determinants capable of facilitating host cell invasion and evasion of immune responses.
Of particular concern is the consistent detection of these virulence genes among strains isolated across different years, with all strains isolated in 2022 testing positive for all virulence genes investigated. This suggests a persistent risk of severe L. monocytogenes infections associated with RTE food consumption over time. Additionally, the identification of unique virulence patterns among different serotypes underscores the complexity of L. monocytogenes pathogenesis and the need for comprehensive surveillance and control measures to mitigate public health risks associated with these pathogens. Overall, the presence of virulence factors such as hlyA, prfA, and inlJ in L. monocytogenes isolates from RTE food products underscores the potential for severe illness in case of contamination.
The current investigation revealed a notable resistance among L. monocytogenes to ampicillin and penicillin, which are commonly used in the treatment of listeriosis. Similar observations of ampicillin and penicillin resistance have been documented in various turkey and chicken meat samples by several researchers [40,41,42].
Conversely, some studies have reported a high susceptibility of L. monocytogenes isolates to these antibiotics [43]. In our study, we found a relatively high proportion of isolates that also exhibited resistance to oxacillin (23.07%). Similarly, in Italy, Pesavento et al. (2010) [44] found a high prevalence of Listeria spp. isolates resistant to methicillin and oxacillin. It is noteworthy that Listeria spp. can transmit or acquire methicillin resistance genes from Enterococcus spp., given that methicillin is commonly employed in the treatment of Enterococcus infections. The antibiotic susceptibility profiles of L. monocytogenes isolates in our study, particularly the high prevalence of resistance to trimethoprim-sulfamethoxazole and oxacillin, have implications for clinical management and treatment strategies for listeriosis cases. Also, the susceptibility of the isolates to certain antibiotics such as ciprofloxacin, levofloxacin, and moxifloxacin provides valuable information for clinicians in selecting appropriate antimicrobial therapy for listeriosis infections, especially in cases of severe illness or systemic involvement.
L. monocytogenes isolated from RTE products found in Romania demonstrated sensitivity to fluoroquinolones (ciprofloxacin, levofloxacin, and moxifloxacin). Compared to other studies, our isolates showed great sensitivity to fluoroquinolones, while others demonstrated a high rate of antimicrobial resistance to fluoroquinolones and tetracycline [42]. The sensitivity of L. monocytogenes isolates to antibiotics commonly used in the treatment of listeriosis (rifampicin, gentamicin, and clindamycin) was to that exhibited in previous studies [42,44]. Chloramphenicol resistance was observed in a small percentage of L. monocytogenes (3.84%). Similar findings have been reported in other studies that were focused on areas where the resistance to this antibiotic may be attributed to its illegal use in veterinary medicine [42]. Moreover, a slightly concerning resistance was noticed to cephalothin (15.38%) which is somehow consistent with previous reports suggesting natural resistance to cephalosporins in Listeria spp. [44].
The prevalence of antibiotic resistance among Listeria spp. has garnered increased attention in recent years. A lot of recent studies have focused on this subject [41,42,44], revealing both multiple resistant strains but also susceptibility to various classes [44], such as gentamicin and chloramphenicol. Our study also revealed a low rate of resistance to gentamicin and chloramphenicol (3.84%). Previous studies [40,42,43] also reported varying percentages of ampicillin-resistant L. monocytogenes strains. Compared to these studies, ours has proved a higher percentage of resistance to ampicillin among L. monocytogenes isolates (19.23%). Our findings suggest an increasing trend in multiresistance among Listeria isolates in RTE products found in Romania.
Resistance among L. monocytogenes isolates was not concentrated according to food products or origin but rather distributed randomly among various types of food products. The presence of a high number of samples positive for resistance genes to tetracycline and beta-lactams, both critical antimicrobial agents in veterinary medicine [45], is concerning. Despite this resistance, L. monocytogenes remains susceptible to primary drugs recommended for treating Listeriosis according to CLSI guidelines [46]. When comparing the results with the ones from the disk diffusion test, we observed disparities between the presence of resistance genes and the phenotypic expression of antibiotic resistance in the tested samples. The results obtained from classical antibiogram testing, which assesses antibiotic susceptibility based on bacterial growth inhibition, did not consistently align with the presence of specific resistance genes. Specifically, there was an obvious difference between the presence of the tetracycline (TET) resistance gene and the actual resistance observed in the samples. While a high percentage (76.92%) of the samples tested positive for the TET resistance gene, only 19.23% exhibited phenotypic resistance to tetracycline. This indicates that although the genetic determinant for tetracycline resistance was prevalent in the samples, the bacteria did not necessarily display resistance when exposed to tetracycline in the laboratory setting. Also, there was a similar discordance in the case of ampicillin resistance. Despite a relatively low number of samples showing phenotypic resistance to ampicillin (only five samples), a substantial proportion (58%) tested positive for the ampC resistance gene associated with ampicillin resistance. These findings indicate the complexity of antibiotic resistance mechanisms in bacteria. The presence of resistance genes does not always guarantee phenotypic resistance, and vice versa. Various factors, including gene regulation, expression levels, and interactions with other genetic elements, can influence the manifestation of antibiotic resistance in bacterial populations. Further research is needed to elucidate the underlying mechanisms driving these discrepancies and their implications for antibiotic resistance surveillance and management strategies [47]. The presence of antimicrobial resistance genes, particularly those associated with tetracycline resistance, highlights the importance of judicious antibiotic use and ongoing surveillance of antimicrobial resistance in L. monocytogenes strains to inform clinical decision-making and ensure effective treatment outcomes.
The heightened resistance to various classes of antibiotics observed in our study could be attributed to the extensive use of animal feed additives and veterinary treatments, which also relates to isolates found in various types of animal-origin food products [48]. Processing procedures during farming practices, slaughtering, and transportation are also critical factors that may contribute to antibiotic resistance in food [49]. Over time, bacteria like Listeria have demonstrated the ability to develop resistance mechanisms through the acquisition of genetic materials from other bacterial species, contributing to the concerning rise in antimicrobial resistance. These findings underscore the urgent need for effective strategies to address antimicrobial resistance and ensure the successful treatment of infectious diseases.

5. Conclusions

This study provides valuable insights into the prevalence, virulence factors, and antibiotic resistance profiles of L. monocytogenes strains isolated from RTE products in the Romanian market. Distinct serotypes among L. monocytogenes isolates were revealed, each with specific virulence gene patterns. The findings reveal concerning levels of resistance, with trimethoprim-sulfamethoxazole and oxacillin showing the highest prevalence of resistance among the tested antibiotics. Conversely, the strains exhibited susceptibility to ciprofloxacin, levofloxacin, and moxifloxacin, indicating potential treatment options for listeriosis infections. Of particular concern is the identification of multidrug resistance in a notable proportion of strains, highlighting the potential challenge in managing infections caused by these bacteria. Additionally, all isolates exhibited multiple antimicrobial resistance genes, with tetracycline resistance genes being the most prevalent. These findings underscore the importance of ongoing surveillance and monitoring of antibiotic resistance in foodborne pathogens to inform public health strategies and combat the emergence of multidrug-resistant strains.

Supplementary Materials

The following supporting information can be downloaded at https://www.mdpi.com/article/10.3390/microorganisms12050954/s1, Figure S1: PCR profile for virulence gene detection; Table S1: Target genes for serogroup, expected size of amplicons and primers used; Table S2: Target genes for antibiotic resistance, expected size of amplicons and primers used. Table S3: Primer sequence, expected product size, and PCR annealing temperature used for amplification.

Author Contributions

Conceptualization, M.N.D. and A.T.; methodology, L.M.C., M.N.D. and S.D.D.; software, O.L.C.-R.; validation, A.T., M.N.D.; formal analysis, O.L.C.-R.; investigation, A.T.; resources, M.N.D.; data curation, A.T. and L.M.C.; writing—original draft preparation, A.T. and M.N.D.; writing—review and editing, A.T.; visualization, M.N.D.; supervision, A.T.; project administration, A.T.; funding acquisition, S.D.D. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Data Availability Statement

The data presented in this study are available on request from the corresponding author due to privacy.

Conflicts of Interest

The authors declare no conflicts of interest.

References

  1. Orsi, R.H.; Wiedmann, M. Characteristics and distribution of Listeria spp., including Listeria species newly described since 2009. Appl. Microbiol. Biotechnol. 2016, 100, 5273–5287. [Google Scholar] [CrossRef] [PubMed]
  2. Matle, I.; Mbatha, K.R.; Madoroba, E. A review of Listeria monocytogenes from meat and meat products: Epidemiology, virulence factors, antimicrobial resistance and diagnosis. Onderstepoort J. Vet. Res. 2020, 87, e1–e20. [Google Scholar] [CrossRef]
  3. Urban-Chmiel, R.; Marek, A.; Stępień-Pyśniak, D.; Wieczorek, K.; Dec, M.; Nowaczek, A.; Osek, J. Antibiotic Resistance in Bacteria—A Review. Antibiotics 2022, 11, 1079. [Google Scholar] [CrossRef] [PubMed]
  4. Roedel, A.; Dieckmann, R.; Brendebach, H.; Hammerl, J.A.; Kleta, S.; Noll, M.; Al Dahouk, S.; Vincze, S. Biocide-Tolerant Listeria monocytogenes Isolates from German Food Production Plants Do Not Show Cross-Resistance to Clinically Relevant Antibiotics. Appl. Environ. Microbiol. 2019, 85, e01253-19. [Google Scholar] [CrossRef]
  5. Guillet, C.; Join-Lambert, O.; Le Monnier, A.; Leclercq, A.; Mechaï, F.; Mamzer-Bruneel, M.F.; Bielecka, M.K.; Scortti, M.; Disson, O.; Berche, P.; et al. Human listeriosis caused by Listeria ivanovii. Emerg. Infect. Dis. 2010, 16, 136–138. [Google Scholar] [CrossRef] [PubMed]
  6. Quereda, J.J.; Morón-García, A.; Palacios-Gorba, C.; Dessaux, C.; García-Del Portillo, F.; Pucciarelli, M.G.; Ortega, A.D. Pathogenicity and virulence of Listeria monocytogenes: A trip from environmental to medical microbiology. Virulence 2021, 12, 2509–2545. [Google Scholar] [CrossRef] [PubMed]
  7. Vázquez-Boland, J.A.; Kuhn, M.; Berche, P.; Chakraborty, T.; Domínguez-Bernal, G.; Goebel, W.; González-Zorn, B.; Wehland, J.; Kreft, J. Listeria pathogenesis and molecular virulence determinants. Clin. Microbiol. Rev. 2001, 14, 584–640. [Google Scholar] [CrossRef] [PubMed]
  8. Jamshidi, A.; Zeinali, T. Significance and Characteristics of Listeria monocytogenes in Poultry Products. Int. J. Food Sci. 2019, 2019, 7835253. [Google Scholar] [CrossRef] [PubMed]
  9. Osek, J.; Wieczorek, K. Listeria monocytogenes—How This Pathogen Uses Its Virulence Mechanisms to Infect the Hosts. Pathogens 2022, 11, 1491. [Google Scholar] [CrossRef]
  10. European Centre for Disease Prevention and Control. The European Union One Health 2022 Zoonoses Report 2022. EFSA J. 2023, 21, 72–74. [Google Scholar]
  11. Zhang, Y.; Yeha, E.; Hallb, G.; Cripeb, J.; Bhagwatc, A.A.; Menget, J. Characterization of Listeria monocytogenes isolated from retail foods. J. Food Microbiol. 2007, 113, 47–53. [Google Scholar] [CrossRef] [PubMed]
  12. Hof, H. An update on the medical management of Listeriosis. Expert. Opin. Pharmacother. 2004, 5, 1727–1735. [Google Scholar] [CrossRef] [PubMed]
  13. Wang, X.M.; Lu, X.F.; Yin, L.; Liu, H.F.; Zhang, W.J.; Si, W. Occurrence and antimicrobial susceptibility of Listeria monocytogenes isolates from retail raw foods. Food Control. 2013, 32, 153–158. [Google Scholar] [CrossRef]
  14. EFSA, European Food Safety Authority European Centre for Disease Prevention and Control. The European Union One Health 2020 Zoonoses Report. EFSA J. 2021, 19, 6971. [Google Scholar]
  15. Borcan, A.M.; Huhulescu, S.; Munteanu, A.; Rafila, A. Listeria monocytogenes—Characterization of strains isolated from clinical severe cases. J. Med. Life 2014, 2, 42–48. [Google Scholar]
  16. SR EN ISO 11290-1:2017; Microbiology of the Food Chain—Horizontal Method for the Detection and Enumeration of Listeria spp. International Standard Organisation: Geneva, Switzerland, 2017.
  17. EUCAST, European Committee on Antimicrobial Susceptibility Testing. Breakpoint Tables for Interpretation of MICs and Zone Diameters, Version 7.0; European Committee on Antimicrobial Susceptibility Testing: Växjö, Sweden, 2017. [Google Scholar]
  18. CLSI. Performance Standards for Antimicrobial Disk Susceptibility Tests, M100S, 29th ed.; CLSI: Wayne, PA, USA, 2019; Volume 39. [Google Scholar]
  19. D’agostino, M.; Wagner, M.; Vazquez-Boland, J.A.; Kuchta, T.; Karpiskova, R.; Hoorfar, J.; Novella, S.; Scortti, M.; Ellison, J.; Murray, A.; et al. A Validated PCR-Based Method to Detect Listeria monocytogenes Using Raw Milk as a Food Model—Towards an International Standard. J. Food Prot. 2004, 67, 1646–1655. [Google Scholar] [CrossRef] [PubMed]
  20. Doumith, M.; Buchrieser, C.; Glaser, P.; Jacquet, C.; Martin, P. Differentiation of the major Listeria monocytogenes serovars by multiplex PCR. J. Clin. Microbiol. 2004, 42, 3819–3822. [Google Scholar] [CrossRef] [PubMed]
  21. Liu, D.; Lawrence, M.L.; Austin, F.W.; Ainsworth, A.J. A multiplex PCR for species- and virulence-specific determination of Listeria monocytogenes. J. Microbiol. Methods 2007, 71, 133–140. [Google Scholar] [CrossRef] [PubMed]
  22. Morvan, A.; Moubareck, C.; Leclercq, A.; Hervé-Bazin, M.; Bremont, S.; Lecuit, M.; Courvalin, P.; Le Monnier, A. Antimicrobial resistance of Listeria monocytogenes strains isolated from humans in France. Antimicrob. Agents Chemother. 2010, 54, 2728–2731. [Google Scholar] [CrossRef]
  23. Mpondo, L.; Ebomah, K.E.; Okoh, A.I. Multidrug-Resistant Listeria Species Shows Abundance in Environmental Waters of a Key District Municipality in South Africa. Int. J. Environ. Res. Public Health 2021, 18, 481. [Google Scholar] [CrossRef]
  24. Srinivasan, V.; Nam, H.; Nguyen, L.; Tamilselvam, B.; Murinda, S.; Oliver, S. Prevalence of antimicrobial resistance genes in Listeria monocytogenes isolated from dairy farms. Foodbourne Pathog. Dis. 2005, 2, 201–211. [Google Scholar] [CrossRef] [PubMed]
  25. Iwu, C.D.; Okoh, A.I. Characterization of Antibiogram Fingerprints in Listeria monocytogenes Recovered from Irrigation Water and Agricultural Soil Samples. PLoS ONE 2020, 15, e0228956. [Google Scholar] [CrossRef] [PubMed]
  26. Rakhmawati, T.W.; Nysen, R.; Aerts, M. Statistical analysis of the Listeria monocytogenes EU-wide baseline survey in certain ready-to-eat foods Part A: Listeria monocytogenes prevalence estimates. EFSA Support. Publ. 2013, 10, EN-441. [Google Scholar] [CrossRef]
  27. Caplan, E.M.; Caplan, D.M.; Ivana, S. Incidence of Listeria species from Food Products. Bull. UASVM Vet. Med. 2011, 68, 1843–5270. [Google Scholar]
  28. Coroneo, V.; Carraro, V.; Aissani, N.; Sanna, A.; Ruggeri, A.; Succa, S.; Sanna, C. Detection of virulence genes and growth potential in Listeria monocytogenes strains isolated from ricotta salata cheese. J. Food Sci. 2016, 81, M114–M120. [Google Scholar] [CrossRef] [PubMed]
  29. Parussolo, L.; Sfaciotte, R.A.P.; Dalmina, K.A.; Melo, F.D.; Da Costa, U.M.; Ferraz, S.M. Detection of virulence genes and antimicrobial susceptibility profile of Listeria monocytogenes isolates recovered from artisanal cheese produced in the Southern region of Brazil. Acad. Bras. Cienc. 2021, 93, e20190200. [Google Scholar]
  30. Almeida, R.M.; Barbosa, A.V.; Lisbôa, R.C.; Santos, A.F.M.; Hofer, E.; Vallim, D.F.; Hofer, C.B. Virulence genes and genetic relationship of L. monocytogenes isolated from human and food sources in Brazil. Braz. J. Infect. Dis. 2017, 21, 282–289. [Google Scholar] [CrossRef] [PubMed]
  31. Iglesias, M.A.; Kroning, I.S.; Decol, L.T.; Franco, B.D.G.M.; Silva, W.P. Occurrence and phenotypic and molecular characterization of Listeria monocytogenes and Salmonella spp. in slaughterhouses in southern Brazil. Food. Res. Int. 2017, 100, 96–101. [Google Scholar] [CrossRef] [PubMed]
  32. Jamali, H.; Radmehr, B.; Thong, K. Prevalence, characterization, and antimicrobial resistance of Listeria species and Listeria monocytogenes isolates from raw milk in farm bulk tanks. Food Control. 2013, 34, 121–125. [Google Scholar] [CrossRef]
  33. Mammina, C.; Aleo, A.; Romani, C.; Pellissier, N.; Nicoletti, P.; Pelcile, P.; Natasi, A.; Pontello, M.M. Characterization of Listeria monocytogenes isolates from human Listeriosis cases in Italy. J. Clin. Microbiol. 2009, 47, 2925–2930. [Google Scholar] [CrossRef]
  34. Oliveira, T.S.; Varjão, L.M.; Silva, L.N.N.; Pereira, R.C.B.; Hofer, E.; Vallim, D.C.; Almeida, R.C.C. Listeria monocytogenes at chicken slaughterhouse: Occurrence, genetic relationship among isolates and evaluation of antimicrobial susceptibility. Food Control. 2018, 88, 131–138. [Google Scholar] [CrossRef]
  35. Orsi, R.H.; Den Bakker, H.C.; Wiedmann, M. Listeria monocytogenes lineages: Genomics, evolution, ecology, and phenotypic characteristics. Int. J. Med. Microbiol. 2011, 301, 79–96. [Google Scholar] [CrossRef] [PubMed]
  36. Van Stelten, A.; Simpson, J.M.; Ward, T.J.; Nightingale, K.K. Revelation by single-nucleotide polymorphism genotyping that mutations leading to a premature stop codon in inlA are common among Listeria monocytogenes isolates from ready-to-eat foods but not human listeriosis cases. Appl. Environ. Microbiol. 2010, 76, 2783–2790. [Google Scholar] [CrossRef] [PubMed]
  37. Jacquet, C.; Doumith, M.; Gordon, J.I.; Martin, P.M.V.; Cossart, P.; Lecuit, M. A Molecular marker for evaluating the pathogenic potential of foodborne Listeria monocytogenes. J. Infect. Dis. 2004, 189, 2094–2100. [Google Scholar] [CrossRef]
  38. Phelps, C.C.; Vadia, S.; Arnett, E.; Tan, Y.; Zhang, X.; Pathak-Sharma, S.; Gavrilin, M.A.; Seveau, S. Relative Roles of Listeriolysin O, InlA, and InlB in Listeria monocytogenes Uptake by Host Cells. Infect. Immun. 2018, 86, e00555-18. [Google Scholar] [CrossRef] [PubMed]
  39. Rajabian, T.; Gavicherla, B.; Heisig, M.; Müller-Altrock, S.; Goebel, W.; Gray-Owen, S.D.; Ireton, K. The bacterial virulence factor InlC perturbs apical cell junctions and promotes cell-to-cell spread of Listeria. Nat. Cell Biol. 2009, 11, 1212–1218. [Google Scholar] [CrossRef] [PubMed]
  40. Ayaz, N.D.; Erol, I. Relation between serotype distribution and antibiotic resistance profiles of Listeria monocytogenes isolated from ground Turkey. J. Food Prot. 2010, 73, 967–972. [Google Scholar] [CrossRef] [PubMed]
  41. Bilir Ormanci, F.S.; Erol, I.; Ayaz, N.D.; Iseri, O.; Sariguzel, D. Immunomagnetic separation and PCR detection of Listeria monocytogenes in turkey meat and antibiotic resistance of the isolates. Brit. Poult. Sci. 2008, 49, 560–565. [Google Scholar] [CrossRef] [PubMed]
  42. Fallah, A.A.; Saei-Dehkordi, S.S.; Rahnama, M.; Tahmasby, H.; Mahzounieh, M. Prevalence and antimicrobial resistance patterns of Listeria species isolated from poultry products marketed in Iran. Food Control. 2012, 28, 327–332. [Google Scholar] [CrossRef]
  43. Aras, Z.; Ardıç, M. Occurrence and Antibiotic Susceptibility of Listeria Species in Turkey Meats. Korean J. Food Sci. Anim. Resour. 2015, 35, 669–673. [Google Scholar] [CrossRef]
  44. Pesavento, G.; Ducci, B.; Nieri, D.; Comodo, N.; Lo Nostro, A. Prevalence and antibiotic susceptibility of Listeria spp. isolated from raw meat and retail foods. Food Control. 2010, 21, 708–713. [Google Scholar] [CrossRef]
  45. Skovgaard, N.; Morgen, C.A. Detection of Listeria spp. in faeces from animals, in feeds, and in raw foods of animal origin. Int. J. Food Microbiol. 1988, 6, 229–242. [Google Scholar] [CrossRef] [PubMed]
  46. Sader, H.; Jones, R. Infection. Impact of EUCAST, CLSI and USCAST ceftaroline breakpoint changes on the susceptibility of methicillin-resistant Staphylococcus aureus isolates collected from US medical centres (2015–2018). J. Clin. Microbiol. 2020, 26, 658–659. [Google Scholar]
  47. Bankole, H.; Semassa, A.; Anihouvi, V.; Dougnon, T.; Legonou, M.; Toukourou, F. Insulation test of Listeria in raw milk in Benin. Int. J. Curr. Microbiol. App. Sci. 2013, 2, 396–400. [Google Scholar]
  48. Mpundu, P.; Mbewe, A.; Muma, J.; Mwasinga, W.; Mukumbuta, N.; Munyeme, M. A global perspective of antibiotic-resistant Listeria monocytogenes prevalence in assorted ready to eat foods: A systematic review. Veter. World 2021, 14, 2219–2229. [Google Scholar] [CrossRef] [PubMed]
  49. Gómez-Laguna, J.; Cardoso-Toset, F.; Meza-Torres, J.; Pizarro-Cerdá, J.; Quereda, J.J. Virulence potential of Listeria monocytogenes strains recovered from pigs in Spain. Vet. Rec. 2020, 187, e101. [Google Scholar] [CrossRef]
Table 1. Antimicrobial resistance genes and virulence characteristics of all strains found in the study.
Table 1. Antimicrobial resistance genes and virulence characteristics of all strains found in the study.
No of Sample/YearSourceSerotypeVirulence Gene DetectedAntimicrobial Resistance Genes Detected
896, 897, 898, 899, 900, 901/2022Pork meat bacon1/2a-3ahlyA, plcB, actA, prfA, inlJTet(M), ampC
669/2019Pork meat sausages1/2a-3chlyA, prfA,Tet(M), Tet(C), Tet(K), dfrD
1018, 1019/2020Fish fillet1/2a-3ahlyA, prfA,ampC, dfrD
830/2020Trout fish butter creme1/2a-3ahlyA, prfA,Tet(M), ampC
102, 103, 104, 105, 106, 107/2022Poultry meat salad1/2a-3ahlyA, plcB, actA, prfA, inlJTet(C), Tet(K), dfrD
93, 94, 95, 96/2022Pork meat hotdog1/2a-3ahlyA, plcB, actA, prfA, inlJampC, dfrD
2018, 2019, 2020, 2021, 2022/2022Beef meat salad1/2bhlyA, plcB, actA, prfA, inlJTet(C), Tet(M), Tet(K)
330/2021Raw milk cheese1/2bhlyA, prfA, inlJTet(K), ampC
Table 2. Antibiotic susceptibility of the L. monocytogenes isolates from RTE food products in Romania.
Table 2. Antibiotic susceptibility of the L. monocytogenes isolates from RTE food products in Romania.
AntimicrobialNo of Resistant L.monocytoegenes Isolate (%)No of Intermediate Resistant L.monocytoegenes Isolate (%)
Ampicillin5 (19.23)2 (7.69%)
Cephalothin4 (15.38%)0
Ciprofloxacin00
Clindamycin1 (3.84%)0
Chloramphenicol1 (3.84%)0
Gentamicin1 (3.84%)0
Levofloxacin00
Moxifloxacin00
Meticillin2 (7.69%)1 (3.84%)
Oxacillin6 (23.07%)2 (7.69%)
Penicillin G4 (15.38%)1 (3.84%)
Rifampicin1 (3.84%)0
Trimethoprime-sulfamethoxazole7 (26.92)1 (3.84%)
Tetracycline5 (19.23)0
Table 3. Resistance patterns in L. monocytogenes isolated from RTE products.
Table 3. Resistance patterns in L. monocytogenes isolated from RTE products.
Multiple Resistance PatternOrigin of StrainResistance PatternNo. of Isolates (%)
One type of antimicrobialFish filletSMX3 (11.53%)
Two types of antimicrobialsPork meatSMX, OXA2 (7.69%)
Trout fishAMP, CEPH2 (7.69%)
Beef meatMET, TET1 (3.84)
Poultry meatPEN, GEN1 (3.84)
Pork meatOXA, CHL1 (3.84)
Three types of antimicrobialsBeef meatAMP, CEPH, TET1 (3.84)
Pork meatAMP, SMX, PEN1 (3.84)
Pork meatCLIN, MET, OXA1 (3.84)
OXA, PEN, TET1 (3.84)
Four types of antimicrobialsBeef meatSMX, CEPH, RIF, TET1 (3.84)
Poultry meatAMP, OXA, PEN, TET1 (3.84)
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Duma, M.N.; Ciupescu, L.M.; Dan, S.D.; Crisan-Reget, O.L.; Tabaran, A. Virulence and Antimicrobial Resistance of Listeria monocytogenes Isolated from Ready-to-Eat Food Products in Romania. Microorganisms 2024, 12, 954. https://doi.org/10.3390/microorganisms12050954

AMA Style

Duma MN, Ciupescu LM, Dan SD, Crisan-Reget OL, Tabaran A. Virulence and Antimicrobial Resistance of Listeria monocytogenes Isolated from Ready-to-Eat Food Products in Romania. Microorganisms. 2024; 12(5):954. https://doi.org/10.3390/microorganisms12050954

Chicago/Turabian Style

Duma, Mihaela Niculina, Laurenţiu Mihai Ciupescu, Sorin Daniel Dan, Oana Lucia Crisan-Reget, and Alexandra Tabaran. 2024. "Virulence and Antimicrobial Resistance of Listeria monocytogenes Isolated from Ready-to-Eat Food Products in Romania" Microorganisms 12, no. 5: 954. https://doi.org/10.3390/microorganisms12050954

APA Style

Duma, M. N., Ciupescu, L. M., Dan, S. D., Crisan-Reget, O. L., & Tabaran, A. (2024). Virulence and Antimicrobial Resistance of Listeria monocytogenes Isolated from Ready-to-Eat Food Products in Romania. Microorganisms, 12(5), 954. https://doi.org/10.3390/microorganisms12050954

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