Core Bacterial Taxa Determine Formation of Forage Yield in Fertilized Soil
Abstract
:1. Introduction
2. Method and Materials
2.1. Site Description
2.2. Experimental Design
2.3. Sampling and Analysis
2.4. Microbial DNA Extraction, Sequencing, Bioinformatics Analysis
2.5. Statistical Analysis
3. Results
3.1. Forage Yield and Soil Properties under Different Fertilization Regimes
3.2. Identifications and Diversity Pattern of Core and Rare Bacterial Taxa
3.3. Ecological Role of Core Bacterial Taxa
3.4. Possible Drivers of Soil Nutrients Cycling and Forage Yield
4. Discussion
4.1. Fertilization Treatments Significantly Changed the Forage Yield and Bacterial Communities
4.2. Organic Manure Addition Enhances Forage Yield by Promoting Core Bacterial Taxa and Influencing Soil C and N Cycling
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Sampling Times | Fertilization | OC (g/kg) | NH4+-N (mg/kg) | NO3−-N (mg/kg) | DOC (mg/kg) | DON (mg/kg) | pH | AP (mg/kg) |
---|---|---|---|---|---|---|---|---|
June | CK | 5.75 ± 0.09 e | 11.51 ± 0.54 c | 1.65 ± 0.08 e | 34.47 ± 3.06 d | 27.04 ± 1.76 e | 8.43 ± 0.11 a | 22.10 ± 1.16 d |
T01 | 20.06 ± 0.53 a | 16.32 ± 0.17 a | 7.27 ± 0.37 a | 180.75 ± 4.85 a | 114.80 ± 3.62 a | 8.41 ± 0.11 a | 58.08 ± 1.64 a | |
T02 | 14.27 ± 0.31 b | 13.75 ± 0.19 b | 5.79 ± 0.18 b | 152.38 ± 19.04 a | 92.41 ± 4.43 b | 8.23 ± 0.08 a | 45.50 ± 1.76 b | |
T03 | 12.37 ± 0.35 c | 13.17 ± 0.26 b | 4.90 ± 0.26 c | 106.54 ± 12.49 b | 81.58 ± 3.42 c | 8.39 ± 0.06 a | 33.52 ± 1.06 c | |
T04 | 9.74 ± 0.38 d | 3.21 ± 0.08 d | 6.7 ± 0.30 a | 75.38 ± 6.78 c | 47.48 ± 0.60 d | 8.22 ± 0.09 a | 30.57 ± 2.90 c | |
T05 | 10.47 ± 0.49 d | 11.55 ± 0.54 c | 3.87 ± 0.02 d | 91.39 ± 2.30 bc | 49.48 ± 3.24 d | 8.33 ± 0.08 a | 31.29 ± 1.40 c | |
August | CK | 5.93 ± 0.31e | 11.23 ± 0.70 c | 1.65 ± 0.06 e | 36.28 ± 1.87 d | 27.72 ± 2.33 d | 8.15 ± 0.08 a | 26.52 ± 1.32 e |
T01 | 20.47 ± 0.19 a | 16.10 ± 0.11 a | 6.22 ± 0.35 a | 183.55 ± 9.52 a | 115.63 ± 4.87 a | 8.38 ± 0.06 a | 60.76 ± 0.79 a | |
T02 | 14.82 ± 0.28 b | 13.27 ± 0.40 b | 5.68 ± 0.30 ab | 154.80 ± 18.72 b | 96.46 ± 3.41 b | 8.26 ± 0.12 a | 46.15 ± 0.92 b | |
T03 | 12.48 ± 0.29 c | 13.41 ± 0.21 b | 5.34 ± 0.35 bc | 146.19 ± 3.99 b | 88.07 ± 3.57 b | 8.38 ± 0.09 a | 34.53 ± 0.77 c | |
T04 | 10.14 ± 0.22 d | 11.13 ± 0.38 c | 3.86 ± 0.04 d | 75.21 ± 4.02 c | 49.89 ± 1.68 c | 8.23 ± 0.11 a | 30.74 ± 1.30 cd | |
T05 | 10.55 ± 0.24 d | 12.58 ± 0.09 b | 4.76 ± 0.13 c | 93.76 ± 7.21 c | 56.15 ± 2.37 c | 8.32 ± 0.09 a | 28.21 ± 2.38 de | |
Mean | CK | 5.84 ± 0.22 e | 11.37 ± 0.6 c | 1.65 ± 0.07 d | 35.38 ± 2.45 e | 27.38 ± 1.98 e | 8.29 ± 0.11 a | 24.31 ± 1.51 e |
T01 | 20.26 ± 0.39 a | 16.21 ± 0.14 a | 6.75 ± 0.41 a | 182.15 ± 7.23 a | 115.22 ± 4.1 a | 8.4 ± 0.08 a | 59.42 ± 1.36 a | |
T02 | 14.55 ± 0.31 b | 13.51 ± 0.32 b | 5.74 ± 0.24 b | 153.59 ± 18.01 b | 94.44 ± 3.87 b | 8.25 ± 0.10 a | 45.82 ± 1.35 b | |
T03 | 12.42 ± 0.31 c | 13.29 ± 0.23 b | 5.12 ± 0.31 b | 126.36 ± 12.23 c | 84.83 ± 3.61 c | 8.39 ± 0.08 a | 34.02 ± 0.91 c | |
T04 | 9.94 ± 0.31 d | 7.17 ± 1.71 d | 5.28 ± 0.64 b | 75.30 ± 5.32 d | 48.69 ± 1.31 d | 8.23 ± 0.10 a | 30.65 ± 2.14 d | |
T05 | 10.51 ± 0.37 d | 12.06 ± 0.43 bc | 4.32 ± 0.21 c | 92.58 ± 5.13 d | 52.82 ± 3.06 d | 8.33 ± 0.08 a | 29.75 ± 1.97 d |
Forage Yield (%) | ||
---|---|---|
Core | ||
Alpha-Richness | 8.63 | |
Alpha-Shannon | 10.55 | |
Beta-PCoA1 | 3.77 | |
Beta-PCoA2 | 3.02 | |
Total | 25.97 | |
Noncore | ||
Alpha-Richness | 2.33 | |
Alpha-Shannon | 1.59 | |
Beta-PCoA1 | 4.22 | |
Beta-PCoA2 | 0.63 | |
Total | 8.77 |
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Wang, X.; Zhao, N.; Li, W.; Pu, X.; Xu, P.; Wang, P. Core Bacterial Taxa Determine Formation of Forage Yield in Fertilized Soil. Microorganisms 2024, 12, 1679. https://doi.org/10.3390/microorganisms12081679
Wang X, Zhao N, Li W, Pu X, Xu P, Wang P. Core Bacterial Taxa Determine Formation of Forage Yield in Fertilized Soil. Microorganisms. 2024; 12(8):1679. https://doi.org/10.3390/microorganisms12081679
Chicago/Turabian StyleWang, Xiangtao, Ningning Zhao, Wencheng Li, Xin Pu, Peng Xu, and Puchang Wang. 2024. "Core Bacterial Taxa Determine Formation of Forage Yield in Fertilized Soil" Microorganisms 12, no. 8: 1679. https://doi.org/10.3390/microorganisms12081679