Microbiome and Microbial Pure Culture Study Reveal Commensal Microorganisms Alleviate Salmonella enterica Serovar Pullorum Infection in Chickens
Abstract
:1. Introduction
2. Materials and Methods
2.1. S. Pullorum Challenge and Sampling
2.2. 16S rRNA Gene Amplicon Sequencing
2.3. Species Annotation and Diversity Analysis
2.4. S. Pullorum Infection Related Microorganisms Isolation and Identification
2.5. Antimicrobial Test
2.6. S. Pullorum Challenge–Intervention Animal Experiment
2.7. Metabolite Detection
2.8. Data Statistics
3. Results
3.1. The Impact of S. Pullorum Challenge on Chicken Performance and Physiology
3.2. S. Pullorum Infection Altered the Richness and Structure of Chicken Gut Microbiota
3.3. Infection Decreased Microorganisms Isolated from Healthy Chicken Feces and Inhibited S. Pullorum Proliferation
3.4. A Lacticaseibacillus paracasei Isolated from Chicken Feces Alleviated S. Pullorum Infection
3.5. Comparative Metabolomic Analysis of Sterile Supernatants from L. paracasei Strains
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Mean (%) | |||
---|---|---|---|
Genus | SP | Ctrl | FC (SP/Ctrl) |
Escherichia-Shigella | 65.0 | 40.6 | 1.6 *** ↑ |
Lactobacillus | 9.86 | 32.1 | 0.31 ** ↓ |
Enterococcus | 10.8 | 3.70 | 2.92 *** ↑ |
Ruminococcus | 0.72 | 1.38 | 0.52 * ↓ |
Streptococcus | 0.16 | 0.83 | 0.19 **↓ |
Bifidobacterium | 0.02 | 0.66 | 0.03 ***↓ |
Ralstonia | 0.14 | 0.47 | 0.29 **↓ |
Clostridium | 0.21 | 0.57 | 0.37 * ↓ |
Butyricicoccus | 0.15 | 0.43 | 0.35 * ↓ |
Coprobacillus | 0.05 | 0.41 | 0.12 **↓ |
Caulobacter | 0.13 | 0.33 | 0.39* ↓ |
Lactococcus | 0.12 | 0.26 | 0.46 * ↓ |
Blautia | 0.04 | 0.16 | 0.25 **↓ |
Veillonella | 0.04 | 0.14 | 0.29 **↓ |
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Zhu, J.; Ding, J.; Yang, K.; Zhou, H.; Yang, W.; Qin, C.; Wang, L.; Xiao, F.; Zhang, B.; Niu, Q.; et al. Microbiome and Microbial Pure Culture Study Reveal Commensal Microorganisms Alleviate Salmonella enterica Serovar Pullorum Infection in Chickens. Microorganisms 2024, 12, 1743. https://doi.org/10.3390/microorganisms12091743
Zhu J, Ding J, Yang K, Zhou H, Yang W, Qin C, Wang L, Xiao F, Zhang B, Niu Q, et al. Microbiome and Microbial Pure Culture Study Reveal Commensal Microorganisms Alleviate Salmonella enterica Serovar Pullorum Infection in Chickens. Microorganisms. 2024; 12(9):1743. https://doi.org/10.3390/microorganisms12091743
Chicago/Turabian StyleZhu, Jianshen, Jinmei Ding, Kaixuan Yang, Hao Zhou, Wenhao Yang, Chao Qin, Liyuan Wang, Fuquan Xiao, Beibei Zhang, Qing Niu, and et al. 2024. "Microbiome and Microbial Pure Culture Study Reveal Commensal Microorganisms Alleviate Salmonella enterica Serovar Pullorum Infection in Chickens" Microorganisms 12, no. 9: 1743. https://doi.org/10.3390/microorganisms12091743