Microorganisms, Volume 12, Issue 9
September 2024 - 188 articles
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Cover Story: Borrelia burgdorferi, the agent of Lyme disease, has been shown to form antimicrobial-resistant biofilms, which protect microorganisms from adverse conditions. Bacterial biofilms are key factors in Lyme disease-related carditis. However, the role of B. burgdorferi biofilms in Lyme carditis remains unexplored due to the absence of a model. In this study, B. burgdorferi-infected mouse heart tissues were examined for biofilms and inflammatory markers using immunohistochemistry (IHC), FISH/IHC, and three-dimensional/atomic force microscopy. The results show that B. burgdorferi forms aggregates with a biofilm marker in mouse heart tissues, inducing inflammation through elevated C-reactive protein levels near biofilms. This mouse model can support future studies on B. burgdorferi biofilms. View this paper