Morphology, Ultrastructure, and Mitochondrial Genome of the Marine Non-Photosynthetic Bicosoecid Cafileria marina Gen. et sp. nov.

Jirsová, Dagmar; Füssy, Zoltán; Richtová, Jitka; Gruber, Ansgar; Oborník, Miroslav

doi:10.3390/microorganisms7080240

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Morphology, Ultrastructure, and Mitochondrial Genome of the Marine Non-Photosynthetic Bicosoecid Cafileria marina Gen. et sp. nov.

by

Dagmar Jirsová

^1,†

,

Zoltán Füssy

^1,2,†

,

Jitka Richtová

^1,2,

Ansgar Gruber

¹

and

Miroslav Oborník

^1,2,*

¹

Institute of Parasitology, Biology Centre, Czech Academy of Sciences, Branišovská 31, 370 05 České Budějovice, Czech Republic

²

Faculty of Science, University of South Bohemia, Branišovská 31, 370 05 České Budějovice, Czech Republic

^*

Author to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Microorganisms 2019, 7(8), 240; https://doi.org/10.3390/microorganisms7080240

Submission received: 26 June 2019 / Revised: 25 July 2019 / Accepted: 1 August 2019 / Published: 5 August 2019

(This article belongs to the Section Systems Microbiology)

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Abstract

In this paper, we describe a novel bacteriophagous biflagellate, Cafileria marina with two smooth flagellae, isolated from material collected from a rock surface in the Kvernesfjorden (Norway). This flagellate was characterized by scanning and transmission electron microscopy, fluorescence, and light microscopy. The sequence of the small subunit ribosomal RNA gene (18S) was used as a molecular marker for determining the phylogenetic position of this organism. Apart from the nuclear ribosomal gene, the whole mitochondrial genome was sequenced, assembled, and annotated. Morphological observations show that the newly described flagellate shares key ultrastructural characters with representatives of the family Bicosoecida (Heterokonta). Intriguingly, mitochondria of C. marina frequently associate with its nucleus through an electron-dense disc at the boundary of the two compartments. The function of this association remains unclear. Phylogenetic analyses corroborate the morphological data and place C. marina with other sequence data of representatives from the family Bicosoecida. We describe C. marina as a new species from a new genus in this family.

Keywords: heterotrophic nano-flagellate; heterokonta; bicosoecida; phylogeny; flagellar apparatus; new genus

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MDPI and ACS Style

Jirsová, D.; Füssy, Z.; Richtová, J.; Gruber, A.; Oborník, M. Morphology, Ultrastructure, and Mitochondrial Genome of the Marine Non-Photosynthetic Bicosoecid Cafileria marina Gen. et sp. nov. Microorganisms 2019, 7, 240. https://doi.org/10.3390/microorganisms7080240

AMA Style

Jirsová D, Füssy Z, Richtová J, Gruber A, Oborník M. Morphology, Ultrastructure, and Mitochondrial Genome of the Marine Non-Photosynthetic Bicosoecid Cafileria marina Gen. et sp. nov. Microorganisms. 2019; 7(8):240. https://doi.org/10.3390/microorganisms7080240

Chicago/Turabian Style

Jirsová, Dagmar, Zoltán Füssy, Jitka Richtová, Ansgar Gruber, and Miroslav Oborník. 2019. "Morphology, Ultrastructure, and Mitochondrial Genome of the Marine Non-Photosynthetic Bicosoecid Cafileria marina Gen. et sp. nov." Microorganisms 7, no. 8: 240. https://doi.org/10.3390/microorganisms7080240

APA Style

Jirsová, D., Füssy, Z., Richtová, J., Gruber, A., & Oborník, M. (2019). Morphology, Ultrastructure, and Mitochondrial Genome of the Marine Non-Photosynthetic Bicosoecid Cafileria marina Gen. et sp. nov. Microorganisms, 7(8), 240. https://doi.org/10.3390/microorganisms7080240

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Morphology, Ultrastructure, and Mitochondrial Genome of the Marine Non-Photosynthetic Bicosoecid Cafileria marina Gen. et sp. nov.

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