Intensification of Nickel Bioleaching with Neutrophilic Bacteria Guyparkeria halophila as an Approach to Limitation of Sulfuric Acid Pollution
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mineral Materials
2.2. Microorganisms and Culturing Media
2.3. Leaching Experiments
2.4. Nickel Analyzes
2.5. Enzyme Activity Analyzes
2.6. Optical Density Analyses
2.7. Statistics
3. Results and Discussion
3.1. Stimulation of Bacterial Growth with Formate
3.2. Stimulation of Ni Leaching by G. halophila with Formic Acid
3.3. Subsequent Leaching with Neutrophilic Bacteria G. halophila and Acidophilic Bacteria Acidithiobacillus sp.
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Nos. | Minerals | Concentration, wt% | |
---|---|---|---|
Name | Composition | ||
1 | pyrrhotite | Fe(1−x)S | 33.0 |
2 | plagioclase | (Na, Ca)AlSi3O8 | 22.0 |
3 | hornblende | Ca2(Mg)O–2(Fe)2–4(Al, Fe)(Si7Al)O22(OH)2 | 13.0 |
4 | hypersthene | (Mg, Fe)SiO3 | 5.0 |
5 | pentlandite | (Fe, Ni)9S8 | 3.0 |
6 | chalcopyrite | CuFeS2 | 0.7 |
Enzyme | Cofactors | Activity, nM/min·mg of Protein | |
---|---|---|---|
A. ferrooxidans ATCC 21834 [14] | G. halophila VKM B-2757D | ||
Formate dehydrogenase | PMS | 80 (max.) | 18 |
NAD+ | 0 | 0 |
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Abashina, T.; Yachkula, A.; Kaparullina, E.; Vainshtein, M. Intensification of Nickel Bioleaching with Neutrophilic Bacteria Guyparkeria halophila as an Approach to Limitation of Sulfuric Acid Pollution. Microorganisms 2021, 9, 2461. https://doi.org/10.3390/microorganisms9122461
Abashina T, Yachkula A, Kaparullina E, Vainshtein M. Intensification of Nickel Bioleaching with Neutrophilic Bacteria Guyparkeria halophila as an Approach to Limitation of Sulfuric Acid Pollution. Microorganisms. 2021; 9(12):2461. https://doi.org/10.3390/microorganisms9122461
Chicago/Turabian StyleAbashina, Tatiana, Alyona Yachkula, Elena Kaparullina, and Mikhail Vainshtein. 2021. "Intensification of Nickel Bioleaching with Neutrophilic Bacteria Guyparkeria halophila as an Approach to Limitation of Sulfuric Acid Pollution" Microorganisms 9, no. 12: 2461. https://doi.org/10.3390/microorganisms9122461